Indian Journal Of Animal Research
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A. Sakthivel Selvan, I.D. Gupta*, Archana Verma, Mahesh V. Chaudhari, Shishir Kanungo and S. Rajesh Kumar
Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India

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Cluster of Differentiation (CD14) protein was identified as a differentiation marker on the surface of monocytes and macrophages and was characterized as a receptor for bacterial endotoxin (LPS). It is regarded as the first described pattern-recognition receptor (PRR) and one of the excellent candidates for mastitis resistance in cattle. Present study was carried out with the objective to characterize and identify genetic polymorphism of promoter region of CD14 gene and its association with clinical mastitis in lactating Karan Fries cattle maintained at NDRI Karnal. Twelve SNPs were found in the complete sequence of 553 base pairs of promoter region of CD14 gene of Karan Fries cows. Cows were also screened using PCR-RFLP with Hpy188I restriction enzyme which revealed three genotypes AA, AB and BB. All three genotypes differed significantly regarding mastitis incidence.
CD14, Karan-Fries, Mastitis, RFLP, SNP.
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