Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Seroprevalence and comparative study of serological tests for detection of mycoplasma gallisepticum infection in commercial layer farms at few districts of Tamil Nadu, India

K. Vadivalagan*, K. Sukumar, V. Sudha Rani1, M Sivachandran2, P. Logesh2, R. Dorairajan

ABSTRACT

Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma gallisepticum (MG). The study was conducted from October 2008 to September 2010 to detect the presence of antibodies against MG in poultry layers. Serum from commercial poultry layers (n = 1350) was tested for MG by using enzyme-linked immunosorbent assay (ELISA) and serum plate agglutination (SPA). A total of 1350 sera from commercial poultry farm were collected and tested in winter, 453 (33.6%) and 350 (26.0%) were positive in SPA test and ELISA, and in summer, 297 (22.0%) and 219 (16.2%) were positive in SPA test and ELISA respectively. In Pearson chi-square test, statistical significant differectnce (p = 0.000) were observed between serological tests (SPA test and ELISA), and it was also demonstrated that seasonal occurrence of infection were higher in zone C. It has been found that MG infection is still an important disease problem in commercial layer farms and there is need for a stringent surveillance and control to combat avian mycoplasmosis in Tamil Nadu and these diagnostic methods (SPA and ELISA) should be only used as screening tools in monitoring programs. 

REFERENCES


  1. Biswas, A., Pramanik, A.K., Mitra, M. and Pal, R.N. (2003). Effect of age, season and managemental practice on the seroprevalence of chronic respiratory disease (CRD) in broilers. Indian J. Ani. Hlth., 42: 161-165.

  2. Butcher, G.D. Factors to consider in serologic testing for Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS), in Electronic Data Information Source (EDIS), Univer-sity of Florida, 2009, VM 126, http://edis.ifas.uû.edu/vm093.

  3. Ewing, M.L., Cookson, K.C, Phillips, R.A, Turner, K.R. and Kleven, S.H. (1998). Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens. Avian Dis. 42: 230.

  4. Godoy, A., Andrade, L.F., Colmenares, O., Bermudez, V., Herrera, A. and Munoz N. (2001). Prevalence of Mycoplasma gallisepticum in egg-laying hens. Vet. Tropi. 26: 25.

  5. Jain, N.C., Chandiramani, N.K. and Singh, I.P. (1971). Studies on avian pleuropneumonic like organisms. Isolation and identification of Mycoplasma gallisepticum. J. Res. Ludhiana., 8: 118-124.

  6. Ley, D.H. (2003) Mycoplasma gallisepticum infection. In Diseases of poultry. Saif, Y.M., Barnes, J.J., Fradley, A.M., Glisson, J.R., McDangald, L.R. and D.E. Swaine. Blackwell Publishing, USA pp. 722.

  7. Mohammed, H.O., Yamamoto, R., Carpenter, T.E. and Ortmayer H.B. (1986). Comparison of egg yolk and serum for the detection of Mycoplasma gallisepticum and M. synoviae antibodies by enzyme-linked immunosorbent assay. Avian Dis., 30: 398.

  8. Office International des Epizooties. (2004) Avian Mycoplasmosis (Mycoplasma gallisepticum). In ‘Manual of standards for diagnostic test and vaccines’. 4th edn, O.I.E., Paris. Chapter, 2.7.3.

  9. Office International des Epizooties. (2008) Animal Health World Organization, “Avian mycoplasmosis,” in Manual of Standards for Diagnostic Tests and Vaccines, chapter 2.3.5, 482.

  10. Peebles, E.D., Parker, T.A., Branton, S.L., Willeford, K.O., Jones, M.S., Gerard, P.D., Pharr, G.T. and Maslin, W.R. (2004) Effects of an S6 strain of Mycoplasma gallisepticum inoculation before beginning of lay on the leukocytic characteristics of commercial layers. Avian Dis. 48: 196.

  11. Pradhan, M.A.M. (2002). Ph.D. Thesis Submitted to the Dept. of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

  12. Sikder, A.J., Islam, M.A., Rahman, M.M. and Rahman, M.B. (2005). Seroprevalence of Salmonella and Mycoplasma gallisetpticum infection in the six model breeder poultry farms at Patuakhili district in Bangladesh. Int. J. Poul. Sci., 4: 905-910.

  13. Taylor-Robinson, D. and Bradbury J.M. (1998). Mycoplasma Diseases, In: A. Balows and M. Sussman. (Eds) Topley and Wilson’s Microbiology and Microbial Infections, 9th edn, Vol. 3, Bacterial infections, Chap. 54, pp. 1013-1037 (London, Edward Arnold).

  14. Zhang, J.H., Bi, D.R., Wang, M.H., Han, B., and Gao, A.X. (2001). Prevalence and pathogenicity of Mycoplasma gallisepticum in broilers in Inner Mongolia. Chinese Journal of Veterinary Science and Technology, 31: 12-13.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)