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Current IssueEditorial BoardOnline First ArticlesArchive

volume 48 issue 6 (december 2014) : 541-544,   Doi: 10.5958/0976-0555.2014.00028.4

INFLUENCE OF AMBIENT TEMPERATURE AND HUMIDITY ON ATP1A1 GENE EXPRESSION IN THARPARKAR AND VRINDAVANI CATTLE

N
N. Kashyap*
P
P. Kumar
B
B. Deshmukh
M
M.S. Dige
M
M. Sarkar
A
A. Kumar
A
A. Chauhan
G
G. Singh
1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 001, India
Cite article:- Kashyap* N., Kumar P., Deshmukh B., Dige M.S., Sarkar M., Kumar A., Chauhan A., Singh G. (2025). INFLUENCE OF AMBIENT TEMPERATURE AND HUMIDITY ON ATP1A1 GENE EXPRESSION IN THARPARKAR AND VRINDAVANI CATTLE. Indian Journal of Animal Research. 48(6): 541-544. doi: 10.5958/0976-0555.2014.00028.4.

ABSTRACT

The Na+/K+-ATPase is responsible for maintenance of the Na+ and K+gradients across the plasma membrane and responds to alteration in oxidative voltage and osmotic changes across membranes. ATP1A1 gene encodes for the a-1 chain of the Na+/K+-ATPase and there exists a positive linear correlation between Na+/K+-ATPase activity and mRNA level of a-1 isoforms. Present study aimed at exploring influence of temperature-humidity variation in winter, spring and summer test-periods on the expression of ATP1A1between Tharparkar and Vrindavani (cross of Holstein Frisian, Jersey and Brown Swiss withHariana) cattle. The value for THI were highest for summer (84.34), followed by spring (68.25) and lowest for winter (52.72),thus spring was consideredas reference season.Vrindavaniand Tharparkar both showed up-regulation of ATP1A1 expression in summer as well as inwinter.Higherup-regulationwas observed in summer. The up-regulation in Vrindavani was significantly higher than Tharparkar in both winter and summer seasons.

REFERENCES

  1. Barada, K., Okolo C., Field M. and Cortas N. (1994). Na, K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon. J. Clin. Invest., 93: 2725.
  2. Jorgensen, P.L., Hakansson K.O. and Karlish S.J.D. (2003). Structure and mechanism of Na, K-ATPase: functional sites and their interactions. Ann. Rev. Physiol., 65: 817-849.
  3. Kashyap, N. (2012). Molecular characterization of ATP1A1 gene and its association with heat tolerance in cattle. MVSc Thesis, Indian Veterinary Research Institute, Izatnagar, Bareilly, India.
  4. Liu, Y.X., Zhou X., Li D.Q., Cui Q.W. and Wang G.L. (2010). Association of ATP1A1 gene polymorphism with heat tolerance traits in dairy cattle. Genet. Mol. Res., 9: 891-896.
  5. Liu, Y.X., Li D.Q., Li H.X., Zhou X. and Wang G.L. (2011). A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows. Mol. Biol. Rep., 38: 83-88.
  6. Marai, I.F.M., El-Darawany A.A., Fadiel A. and Abdel-Hafez M.A.M. (2007). Physiological traits as affected by heat stress in sheep-A review. Small Ruminant Res., 71: 1-12.
  7. McDowell, R.E., Hooven N.W. and Camoens J.K. (1976). Effect of climate on performance of Holsteins in first lactation. J. Dairy Sci., 59: 965-971.
  8. Morel, P., Tallineau C., Pontcharraud R., Piriou A. and Huguet F. (1998). Effects of 4-hydroxynonenal, a lipid peroxidation product, on dopamine transport and Na+/K+ ATPase in rat striatal synaptosomes. Neurochem. Int., 33: 531-540.
  9. Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res., 29: e45-e45.
  10. Ravagnolo, O., Misztal I. and Hoogenboom G. (2000). Genetic component of heat stress in dairy cattle, development of heat index function. J. Dairy Sci., 83: 2120-2125.
  11. Rolfe, D.F. and Brown G.C. (1997). Cellular energy utilization and molecular origin of standard metabolic rate in mammals. Physiol. Rev., 77: 731-758.
  12. Shelton, M. (2000). Reproductive performance of sheep exposed to hot environments. In: Sheep production in hot and arid zones, pp. 155-162. Kuwait Institute for Scientific Research, Kuwait.
  13. Sonna, L.A., Fujita J., Gaffin S.L. and Lilly C.M. (2002). Invited review: effects of heat and cold stress on mammalian gene expression. J. Appl.Physiol., 92: 1725-1742.
  14. Tsimarato, M., Coste T.C., Djemli-Shipkolye A., Daniel L., Shipkolye F., Vague P. and Raccah D. (2001). Evidence of time-dependent changes in renal medullary Na, K-ATPase activity and expression in diabetic rats. Cell. Mol. Biol., 47: 239-245.
  15. Vague, P., Dufayet D., Coste T., Moriscot C., Jannot M.F. and Raccah D. (1997). Association of diabetic neuropathy with Na/K ATPase gene polymorphism. Diabetologia, 40: 506-511.
  16. Wang, Z., Wang G., Huang J., Li Q., Wang C. and Zhong J. (2011). Novel SNPs in the ATP1B2 gene and their associations with milk yield, milk composition and heat-resistance traits in Chinese Holstein cows. Mol. Biol. Rep., 38: 1749-1755.
  17. Xu, K.Y. (2005). Activation of (Na+/K+)-ATPase. Biochem. Biophys. Res. Comm., 338: 1669-1677.
  18. Yang, S. (2007). Study on seasonal variation on some biochemical indices of blood and correlation between them and heat tolerance in Holstein cows. Master’s Thesis, Xinjiang Agriculture University, Ürümqi, Xinjiang.
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Indian Journal of Animal Research
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    volume 48 issue 6 (december 2014) : 541-544,   Doi: 10.5958/0976-0555.2014.00028.4

    INFLUENCE OF AMBIENT TEMPERATURE AND HUMIDITY ON ATP1A1 GENE EXPRESSION IN THARPARKAR AND VRINDAVANI CATTLE

    N
    N. Kashyap*
    P
    P. Kumar
    B
    B. Deshmukh
    M
    M.S. Dige
    M
    M. Sarkar
    A
    A. Kumar
    A
    A. Chauhan
    G
    G. Singh
    1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 001, India
    Cite article:- Kashyap* N., Kumar P., Deshmukh B., Dige M.S., Sarkar M., Kumar A., Chauhan A., Singh G. (2025). INFLUENCE OF AMBIENT TEMPERATURE AND HUMIDITY ON ATP1A1 GENE EXPRESSION IN THARPARKAR AND VRINDAVANI CATTLE. Indian Journal of Animal Research. 48(6): 541-544. doi: 10.5958/0976-0555.2014.00028.4.

    ABSTRACT

    The Na+/K+-ATPase is responsible for maintenance of the Na+ and K+gradients across the plasma membrane and responds to alteration in oxidative voltage and osmotic changes across membranes. ATP1A1 gene encodes for the a-1 chain of the Na+/K+-ATPase and there exists a positive linear correlation between Na+/K+-ATPase activity and mRNA level of a-1 isoforms. Present study aimed at exploring influence of temperature-humidity variation in winter, spring and summer test-periods on the expression of ATP1A1between Tharparkar and Vrindavani (cross of Holstein Frisian, Jersey and Brown Swiss withHariana) cattle. The value for THI were highest for summer (84.34), followed by spring (68.25) and lowest for winter (52.72),thus spring was consideredas reference season.Vrindavaniand Tharparkar both showed up-regulation of ATP1A1 expression in summer as well as inwinter.Higherup-regulationwas observed in summer. The up-regulation in Vrindavani was significantly higher than Tharparkar in both winter and summer seasons.

    REFERENCES

    1. Barada, K., Okolo C., Field M. and Cortas N. (1994). Na, K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon. J. Clin. Invest., 93: 2725.
    2. Jorgensen, P.L., Hakansson K.O. and Karlish S.J.D. (2003). Structure and mechanism of Na, K-ATPase: functional sites and their interactions. Ann. Rev. Physiol., 65: 817-849.
    3. Kashyap, N. (2012). Molecular characterization of ATP1A1 gene and its association with heat tolerance in cattle. MVSc Thesis, Indian Veterinary Research Institute, Izatnagar, Bareilly, India.
    4. Liu, Y.X., Zhou X., Li D.Q., Cui Q.W. and Wang G.L. (2010). Association of ATP1A1 gene polymorphism with heat tolerance traits in dairy cattle. Genet. Mol. Res., 9: 891-896.
    5. Liu, Y.X., Li D.Q., Li H.X., Zhou X. and Wang G.L. (2011). A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows. Mol. Biol. Rep., 38: 83-88.
    6. Marai, I.F.M., El-Darawany A.A., Fadiel A. and Abdel-Hafez M.A.M. (2007). Physiological traits as affected by heat stress in sheep-A review. Small Ruminant Res., 71: 1-12.
    7. McDowell, R.E., Hooven N.W. and Camoens J.K. (1976). Effect of climate on performance of Holsteins in first lactation. J. Dairy Sci., 59: 965-971.
    8. Morel, P., Tallineau C., Pontcharraud R., Piriou A. and Huguet F. (1998). Effects of 4-hydroxynonenal, a lipid peroxidation product, on dopamine transport and Na+/K+ ATPase in rat striatal synaptosomes. Neurochem. Int., 33: 531-540.
    9. Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res., 29: e45-e45.
    10. Ravagnolo, O., Misztal I. and Hoogenboom G. (2000). Genetic component of heat stress in dairy cattle, development of heat index function. J. Dairy Sci., 83: 2120-2125.
    11. Rolfe, D.F. and Brown G.C. (1997). Cellular energy utilization and molecular origin of standard metabolic rate in mammals. Physiol. Rev., 77: 731-758.
    12. Shelton, M. (2000). Reproductive performance of sheep exposed to hot environments. In: Sheep production in hot and arid zones, pp. 155-162. Kuwait Institute for Scientific Research, Kuwait.
    13. Sonna, L.A., Fujita J., Gaffin S.L. and Lilly C.M. (2002). Invited review: effects of heat and cold stress on mammalian gene expression. J. Appl.Physiol., 92: 1725-1742.
    14. Tsimarato, M., Coste T.C., Djemli-Shipkolye A., Daniel L., Shipkolye F., Vague P. and Raccah D. (2001). Evidence of time-dependent changes in renal medullary Na, K-ATPase activity and expression in diabetic rats. Cell. Mol. Biol., 47: 239-245.
    15. Vague, P., Dufayet D., Coste T., Moriscot C., Jannot M.F. and Raccah D. (1997). Association of diabetic neuropathy with Na/K ATPase gene polymorphism. Diabetologia, 40: 506-511.
    16. Wang, Z., Wang G., Huang J., Li Q., Wang C. and Zhong J. (2011). Novel SNPs in the ATP1B2 gene and their associations with milk yield, milk composition and heat-resistance traits in Chinese Holstein cows. Mol. Biol. Rep., 38: 1749-1755.
    17. Xu, K.Y. (2005). Activation of (Na+/K+)-ATPase. Biochem. Biophys. Res. Comm., 338: 1669-1677.
    18. Yang, S. (2007). Study on seasonal variation on some biochemical indices of blood and correlation between them and heat tolerance in Holstein cows. Master’s Thesis, Xinjiang Agriculture University, Ürümqi, Xinjiang.
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