Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 50 issue 1 (february 2016) : 8-13

Computer assisted sperm analysis of fresh and frozen-thawed buffalo semen and their interrelationship

J.B. Patel, A.J. Dhami*
1<p>College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand-388 001, Gujarat, India&nbsp;</p>
Cite article:- Patel J.B., Dhami* A.J. (NaN). Computer assisted sperm analysis of fresh and frozen-thawed buffalo semen and their interrelationship . Indian Journal of Animal Research. 50(1): 8-13. doi: 10.18805/ijar.8559.

ABSTRACT

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.37±1.58%) were highly significantly (Pand medium motile sperm (%) increased, while beat-cross frequency (Hz), straightness (%), linearity (%), sperm area (µm2) and rapidly motile sperm (%) decreased significantly in post-thawed sperms when compared with the fresh sperm of both Jafarabadi and Mehsana bulls. The initial motility and live sperm per cent were significantly correlated with CASA traits of fresh and frozen-thawed semen, and all the sperm motility and velocity traits of fresh and frozen-thawed semen assessed by CASA were significantly interrelated among both the breeds. The interrelationships were stronger in Mehsana bulls as compared to Jafarabadi bulls.

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