Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Indian Journal of Animal Research, volume 50 issue 1 (february 2016) : 140-141

Gastrin peptide isolation in broiler chicken

A.K.Mandal*, R.K.Das, A.Maity1, G. R.Sahoo
1<p>Department of Anatomy &amp; Histology, College of Veterinary Science &amp; A.H., Orissa University of Agriculture &amp; Technology, Bhubaneswar&ndash;751 003, India</p>
Cite article:- A.K.Mandal*, R.K.Das, A.Maity1, R.Sahoo G. (NaN). Gastrin peptide isolation in broiler chicken . Indian Journal of Animal Research. 50(1): 140-141. doi: 10.18805/ijar.6694.

ABSTRACT

The present study was undertaken to isolate gastrin peptide from the antral tissue of broiler chicken. The chicken antrums, i.e. tissue pieces from a narrow zone at gizzard – duodenal junction were collected, boiled in distilled water, followed by centrifugation at 0º C. The supernatant was collected, added to isopropanol and stirred overnight. After addition of dichloromethane, the aqueous phase was partitioned, aspirated and lyophilized. The electrophoretic analysis (SDS-PAGE) of the antral sample was carried out after running it along with a reference protein marker. Characterization of the antral extract revealed a total of eleven peptide bands having relative molecular weights (Mr) ranging from 4.6 to114.5 kDa, out of which peptides having Mr of 22.6 and 26.3 kDa were major ones. The protein or peptide band showing the lowest relative molecular weight (Mr, 4.6 kDa) was identified as the gastrin.

REFERENCES


  1. Aksoy, A. and Cinar, K. (2009). Distribution and ontogeny of gastrin- and serotonin- immunoreactive cells in the proventriculus of developing chick, Gallus gallus domesticus. J. Vet. Sci., 10(1):9-13.

  2. Dimaline, R. and Lee, C. M. (1990). Chicken gastrin: a member of gastrin/ CCk family with novel structure-activity relationships. Am.J.Physiol., 259: G882-888.

  3. Dimaline, R.; Young, J. and Gregory, H. (1986). Isolation from chicken antrum, and primary amino acid sequence of a novel 36-residue peptide of the gastrin/CCK family. FEBS Letters 4003, 205(2): 318 – 322.

  4. Gregory, R.A.; Tracy, H.J.; Grossman, M.I.; Valois, D.D. and Lichter, R. (1969). Isolation of canine gastrin. Experientia, 25 (4): 345 – 346.

  5. Laemmli, U.K.(1970).Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227(5259): 680-685.

  6. Larsson, L.I. (2000).Developmental biology of gastrin and somatostatin cells in the antropyloric mucosa of the stomach. Microsc. Res. Tech., 48: 272- 281.

  7. Larsson, L.I.; Sundler, F.; Hakanson, R.; Rehfeld, J.F. and Stadil, F. (1974). Distribution and properties of gastrin cells in the gastrointestinal tract of chicken. Cell Tiss. Res., 154: 409 – 421.

  8. Lowry, O.H.; Rosebrough, N.J.; Farr, A.B. and Randall, R.J. (1951). Protein measurement with the Folin-phenol reagent. J. Biol. Chem., 193: 265.

  9. Sturkie, P.D. and Whittow, G.C. (2000). Gastrointestinal anatomy and physiology. In: Sturkie’s Avian Physiology, 5th edition, Academic Press, NY, pp. 299- 321.

  10. Taubert, S. and Madison, L.(1965). The Isolation and Characterization of Porcine Gastrin. J. Biol. Chem., 240 (2): 645 – 650.

  11. Trampel, D.W. and Duke, G.E. (2004). Avian digestion. In : Dukes’ Physiology of Domestic Animals, (ed.) Reece, W.O. 12th edition, Panima Publishing Corporation, New Delhi and Bangalore, pp.488- 500.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)