Molecular characterization of promoter of IGF-1 gene in chicken broiler line and its growth performance

DOI: 10.18805/ijar.8417    | Article Id: B-2774 | Page : 23-26
Citation :- Molecular characterization of promoter of IGF-1 gene in chicken broilerline and its growth performance .Indian Journal Of Animal Research.2016.(50):23-26

C. Paswan*, T.K. Bhattacharya, R.N. Chatterjee , C.S. Nagaraja and K. Dushyanth

drcdvet17@gmail.com
Address :

ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad-500 030, India

Abstract

A study was carried out to characterize the nucleotide variability in the promoter of the IGF-1 gene in broiler line of chicken. A PCR product of 375bp was amplified and nucleotide variability was studied using PCR-SSCP technique in chicken control broiler line. Selected sample PCR products were also sequenced to confirm the variability in promoter sequence. Present study revealed that the IGF-1 promoter was monomorphic having similar SSCP pattern in all individuals. Growth data was also analyzed to study the growth performance of the chicken broiler line at different age. Growth performance of male and female differed significantly at six week of age.  

Keywords

Chicken Growth performance IGF-1 promoter Nucleotide variability.

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