Kit Ligand Expression in granulosa cells during follicular culture and associated maturation rate of oocytes in buffalo (Bubalus bubalis)

DOI: 10.18805/ijar.7488    | Article Id: B-2772 | Page : 14-18
Citation :- Kit Ligand Expression in granulosa cells during follicular culture and associated maturation rate of oocytes in buffalo (Bubalus bubalis) .Indian Journal Of Animal Research.2016.(50):14-18

Atul Mahajan*, Parveen Kumar, Sachin Atole, B P Brahmkshtri1, K. R. Tajane2, Sachinandan De, Tirtha Kumar Datta and S. L. Goswami

dratulmahajan@gmail.com
Address :

Animal Biotechnology Centre, National Dairy Research Institute, Karnal-132 001, India.

Abstract

The objectives of the study to associate the expression of Kit Ligand gene under different gonadotropins and growth factors supplementation with the maturation rate of oocytes in buffalo  ovarian follicular cells during whole follicular culture at subsequent intervals of 24 hours. Intact ovarian follicles were dissected out of buffalo ovaries and cultured in Tissue Culture Media-199 supplemented with gonadotropins and growth factors for 24 hours. A semi-quantitative RT-PCR amplification was obtained in granulose cells of buffalo ovarian follicles and its expression was found to be restricted during initial stage of follicle culture. KL expression persistency in granulosa cells beyond 4 hours during follicular culture was found to be negatively correlated with maturation rate of oocytes. It was concluded that expression of Kit Ligand gene in granulose cells could be predictive role for assessing oocytes maturation rate and possibly the oocytes competence in buffalo.

Keywords

Buffalo Follicle culture Kit Ligand (KL) gene expression Oocyte maturation.

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