Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 49 issue 5 (october 2015) : 618-622

Cloning and sequence analysis of b-tubulin gene of Trypanosoma evansi isolates from Indian dromedaries (Camelus dromedarius)

M. Ashraf, Meera Srivastava, S.K. Ghorui, G. Nagarajan, Shelesh kumar Swami, Sanjay Kumar
1National Research Centre on Camel, Bikaner-334 001, India
Cite article:- Ashraf M., Srivastava Meera, Ghorui S.K., Nagarajan G., Swami kumar Shelesh, Kumar Sanjay (2024). Cloning and sequence analysis of b-tubulin gene of Trypanosoma evansi isolates from Indian dromedaries (Camelus dromedarius). Indian Journal of Animal Research. 49(5): 618-622. doi: 10.18805/ijar.5573.

ABSTRACT

Trypanosomosis caused by Trypanosoma evansi is a disease of economic significance of domestic livestock, causing trypanosomiasis, commonly known as Surra. In the present study, gene of Trypanosoma evansi have beer cloned and sequenced. Beta-tubulin is important for cellular structure and physical functions. Sequence analysis of this gene will be useful for the vaccine studies. The open reading frame of b-tubulin of Trypanosoma evansi is 1329 bp in length, encoding 442 amino acid polypeptide. The sequence analysis revealed that the ß tubulin gene of Trypanosoma evansi from India was having the identity of 99% with T.equiperdum, T.evansi (China isolate), T.evansi (Indian isolates), T.brucei and 88% with T.cruzi. The stable biological characteristics of Trypanosomatidae beta-tubulin make it an ideal target of many anti-parasitic drugs that block tubulin function.

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