Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 49 issue 6 (december 2015) : 851-854

Effect of different extenders on quality of frozen-thawed boar semen

Frydrychová S*, Lustyková A, Václavková E, Lipenský J, Rozkot M
1<p>Department of &nbsp;Pig Breeding, Praha - Uhr&iacute;neves, Workplace Kostelec nad Orlic&iacute;,&nbsp;Komensk&eacute;ho 1239, 517 41 Kostelec nad Orlic&iacute;, Czech Republic.</p>
Cite article:- S* Frydrychov&aacute;, A Lustykov&aacute;, E V&aacute;clavkov&aacute;, J Lipensk&yacute;, M Rozkot (NaN). Effect of different extenders on quality of frozen-thawed boar semen . Indian Journal of Animal Research. 49(6): 851-854. doi: 10.18805/ijar.7050.

ABSTRACT

The objective of this study was to investigate the effect of using different extenders viz. Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed.  Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

REFERENCES


  1. Bielas W., Dubiel A., Nizanski W., (2003): Effects of cryopreservation methods, packaging systems and the thermoresistance test on the post-thaw quality of boar semen. Med. Weter., 59: 172-175.

  2. Cerolini S., Maldjian A., Pizzi F. and Gliozzi T.M., (2001): Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction, 121: 395-401.

  3. Cerovský J., (1976): Metoda barvení kancích spermií pro morfologické hodnocení. Živocišná Výroba, 21: 361-366.

  4. Fischer P.S., (1991): Interactions of cooling velocity, warming velocity and glycerol concentration on the survival of frozen-thawed boar sperm. In: Boar semen preservation II. Johnson L.A., Rath D., (eds.), Proc. of the 2nd International Congress on Boar Semen Preservation, Beltsville. Reprod. Dom. Anim., 1: 123-137.

  5. Gila M. A., Roca J., Cremades T., Hernández M., Vázquez J.M., Rodríguez-Martínez H., Martínez E.A., (2005): Does multivariate analysis of post-thaw sperm characteristics accurately estimate in vitro fertility of boar individual ejaculates? Theriogenology, 64: 305-316.

  6. Gutiérrez-Pérez O., Juárez-Mosqeda M.L., Carvajal S.U., Ortega M.E.T., (2009): Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58: 287-292.

  7. Frydrychova S., Lustyková A., Václavková E., Lipenský J., Rozkot M., (2014): Seasonal changes in fresh semen quality and freezability in boar semen. Indian J Anim. Sci., 84: 643-646.

  8. Frydrychová S., Cerovský J., Lustyková A., Rozkot M., (2010): Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen. Czech J. Anim. Sci., 55: 160-166.

  9. Funahashi H., Sano T., (2005): Select antioxidants improve the function of extended boar semen stored at 10 degrees C. Theriogenology, 63: 1605-1616.

  10. Gadea J., (2003): Review: semen extender used in the artificial insemination of swine. Span. J. Agric. Res., 1: 17-27.

  11. Holt W.V., Medrano A., Thurston L.M., Watson P. F., (2005): The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology, 63: 370-382.

  12. Johnson L.A., Weitze K.F., Fiser P., Maxwell W.M.C., (2000): Storage of boar semen. Anim. Reprod. Sci., 62: 143-172.

  13. Kaeoket K., Chanapai P., Junchiyaphoom P., Chanapiwat P., (2011): The effect of using long term and short term extenders during cooling process on the quality of frozen boar semen. Thai J. Vet. Med., 41: 283-288.

  14. Knox R.V., (2011): The current value of frozen-thawed boar semen for commercial companies. Reprod. Domest. Anim., 46: 4-6.

  15. Lalrintluanga K., Deka B.C., Nath K.C., Bhuyan D., Ali M.A. and Sarma S., (2012): Effect of extenders on the extracellular activity of transaminases in boar semen during preservation. Indian J. Anim. Res., 46: 295-297.

  16. Larson K., Einarsson S., Nicander L., (1996): Influence of thawing diluents on vitality, acrosome morphology ultrastructure and enzyme release on deep frozen boar spermatozoa. Livest. Prod. Sci., 5: 293.

  17. Pandey R.P., Singh B.K., (2001): Effect of dilutors on extracellular enzyme activity of preserved boar semen. Indian J. Anim. Sci., 71: 826-828.

  18. Roca J., Hernández M., Carvajal G., Vázquez J.M., Martínez E.A., (2006): Factors influencing boar sperm cryosurvival. J. Anim. Sci., 84: 2692-2699.

  19. Safranski T.J., Ford J.J., Rohrer G.A. and Guthrie H.D., (2011): Plenary contribution to International Conference on Boar Semen Preservation 2011. Genetic selection for freezability and its controversy with selection for performance. Reprod. Dom. Anim., 46: 31-34.

  20. Westendorf P., Richter L. and Treu H., (1975): Zur Tiefgefrierung von Ebersperma. Labor- und Besamungsergebnisse mit dem Hulsenberger Paillettenverfahren. Deutsche Tierarzliche Wochenschrift, 82: 261-267. 
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