Effect of different extenders on quality of frozen-thawed boar semen

DOI: 10.18805/ijar.7050    | Article Id: B-272 | Page : 851-854
Citation :- Effect of different extenders on quality of frozen-thawed boar semen .Indian Journal Of Animal Research.2015.(49):851-854

Frydrychová S*, Lustyková A, Václavková E, Lipenský J and Rozkot M

frydrychova.sona@vuzv.cz
Address :

Department of  Pig Breeding, Praha - Uhríneves, Workplace Kostelec nad Orlicí, Komenského 1239, 517 41 Kostelec nad Orlicí, Czech Republic.

Abstract

The objective of this study was to investigate the effect of using different extenders viz. Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed.  Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

Keywords

Boar semen Cryopreservation Extenders Semen quality.

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