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volume 49 issue 5 (october 2015) : 709-712,   Doi: 10.18805/ijar.5587

Diagnosis of dermatophilosis in dairy cattle in Kerala, India

P
P. V. Tresamol
M
M. R. Saseendranath
1College of Veterinary and Animal Sciences, Mannuthy,Thrissur-680 651, India.
Cite article:- Tresamol V. P., Saseendranath R. M. (2025). Diagnosis of dermatophilosis in dairy cattle in Kerala, India. Indian Journal of Animal Research. 49(5): 709-712. doi: 10.18805/ijar.5587 .

ABSTRACT

Skin scabs and scrapings from 82 dermatitis cases in dairy cattle were subjected to detailed bacteriological, mycological, parasitological and molecular studies. Microscopical examination of Giemsa or Gram’s stained smears of scab material from the lesions revealed characteristic gram positive septate branching filaments with typical tram track appearance suggestive of Dermatophilus congolensis in 72 samples (91.5%). Culture of scab materials in sheep blood agar under anaerobic condition yielded typical beta haemolytic colonies of D. congolensis in 75 samples, which were further confirmed by colony morphology, staining characters and biochemical reactions. Molecular confirmation of the isolates was carried out using polymerase chain reaction with primers based on 16S rRNA which yielded specific band of 500bp. The pathogenicity of the isolates was also proved by experimental inoculation into rabbits.

REFERENCES

  1. Amor, A., Enriquenz, A., Corcuera, M. T., Toro, C., Herroro, D. and Baquero, M. (2011). Is infection by Dermatophilus congolensis underdiagnosed? J. Clin. Microbiol. 49: 449-451.
  2. Award, W. S., Nadra-Elwgoud, Abdou, M. I. and El-Sayed, A. A. (2008). Diagnosis and treatment of bovine, ovine, equine dermatophilosis. J. Appl. Sci. Res. 4: 367-374
  3. Barrow G.I., and Feltham R.K.A. (1993) Cowan and Steel’s Manual for Identification of Medical Bacteria. 3rd edition. Cambridge University Press, New York. p 331
  4. Haalstra, R.T. (1965). Isolation of Dermatophilus congolensis from skin lesions in the diagnosis of streptothricosis. Vet. Rec. 77: 824-825
  5. Hashemi Tabar, G. R., Rad, M. and Chavoshi, M. (2004). A survey on dermatophilosis in sheep in the north of Iran. Iranian J. Vet. Res. 5: 97-101
  6. Mannan, M. A., Khan, M. S. R., Rahman, M. M., Begum, F. and Uddin, M. Z. (2009). Isolation and identification of dermatophilus bacteria from the skin lesions of cattle. Bangladesh J. Vet. Med. 7: 342-347
  7. Nath, B. D., Ahasa, M. S., Rehman, M. S. and Haque, A. K. M. F. (2010). Prevalence and therapeutic management of bovine dermatophilosis. Bangaladesh Res. Pub. J. 4: 198-207
  8. Pal, M. (1995). Prevalence in India of Dermatophilus congolensis infection in clinical specimens from animals and humans. Rev. Sci. Tech. Off. Int. Epiz. 14: 857-863
  9. Quinn, P. J., Carter, M. E., Markey, B. K. and Carter, G. R. (1994). Clinical Veterinary Microbiology. Wolfe Publishing.USA. p 648
  10. Salkin, I. F., Stone, W. B. and Gordon, M. A. (1981). Dermatophilus congolensis infections in wild life in New York state. J. Clin. Microbiol. 14: 604-606
  11. Shaibu, S. J., Kazeem, H. M., Abdullahi, U. S., Fatihu, M. Y. (2010). Use of polymerase chain reaction in the diagnosis of dermatophilosis from cattle, sheep and goats in Nigeria. J. Anim.Vet.Adv. 9: 1034-1036
  12. Wen-Xing, H., Yu, C., Jingmei, W., Yuvanzhi, W. and Gen-Quiang, Y. (2007). Establishment of polymerase chain reaction assay for detection of dermatophilosis in sheep. . http://www.paper.edu.cn/en/paper.serial number 200704-270
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    volume 49 issue 5 (october 2015) : 709-712,   Doi: 10.18805/ijar.5587

    Diagnosis of dermatophilosis in dairy cattle in Kerala, India

    P
    P. V. Tresamol
    M
    M. R. Saseendranath
    1College of Veterinary and Animal Sciences, Mannuthy,Thrissur-680 651, India.
    Cite article:- Tresamol V. P., Saseendranath R. M. (2025). Diagnosis of dermatophilosis in dairy cattle in Kerala, India. Indian Journal of Animal Research. 49(5): 709-712. doi: 10.18805/ijar.5587 .

    ABSTRACT

    Skin scabs and scrapings from 82 dermatitis cases in dairy cattle were subjected to detailed bacteriological, mycological, parasitological and molecular studies. Microscopical examination of Giemsa or Gram’s stained smears of scab material from the lesions revealed characteristic gram positive septate branching filaments with typical tram track appearance suggestive of Dermatophilus congolensis in 72 samples (91.5%). Culture of scab materials in sheep blood agar under anaerobic condition yielded typical beta haemolytic colonies of D. congolensis in 75 samples, which were further confirmed by colony morphology, staining characters and biochemical reactions. Molecular confirmation of the isolates was carried out using polymerase chain reaction with primers based on 16S rRNA which yielded specific band of 500bp. The pathogenicity of the isolates was also proved by experimental inoculation into rabbits.

    REFERENCES

    1. Amor, A., Enriquenz, A., Corcuera, M. T., Toro, C., Herroro, D. and Baquero, M. (2011). Is infection by Dermatophilus congolensis underdiagnosed? J. Clin. Microbiol. 49: 449-451.
    2. Award, W. S., Nadra-Elwgoud, Abdou, M. I. and El-Sayed, A. A. (2008). Diagnosis and treatment of bovine, ovine, equine dermatophilosis. J. Appl. Sci. Res. 4: 367-374
    3. Barrow G.I., and Feltham R.K.A. (1993) Cowan and Steel’s Manual for Identification of Medical Bacteria. 3rd edition. Cambridge University Press, New York. p 331
    4. Haalstra, R.T. (1965). Isolation of Dermatophilus congolensis from skin lesions in the diagnosis of streptothricosis. Vet. Rec. 77: 824-825
    5. Hashemi Tabar, G. R., Rad, M. and Chavoshi, M. (2004). A survey on dermatophilosis in sheep in the north of Iran. Iranian J. Vet. Res. 5: 97-101
    6. Mannan, M. A., Khan, M. S. R., Rahman, M. M., Begum, F. and Uddin, M. Z. (2009). Isolation and identification of dermatophilus bacteria from the skin lesions of cattle. Bangladesh J. Vet. Med. 7: 342-347
    7. Nath, B. D., Ahasa, M. S., Rehman, M. S. and Haque, A. K. M. F. (2010). Prevalence and therapeutic management of bovine dermatophilosis. Bangaladesh Res. Pub. J. 4: 198-207
    8. Pal, M. (1995). Prevalence in India of Dermatophilus congolensis infection in clinical specimens from animals and humans. Rev. Sci. Tech. Off. Int. Epiz. 14: 857-863
    9. Quinn, P. J., Carter, M. E., Markey, B. K. and Carter, G. R. (1994). Clinical Veterinary Microbiology. Wolfe Publishing.USA. p 648
    10. Salkin, I. F., Stone, W. B. and Gordon, M. A. (1981). Dermatophilus congolensis infections in wild life in New York state. J. Clin. Microbiol. 14: 604-606
    11. Shaibu, S. J., Kazeem, H. M., Abdullahi, U. S., Fatihu, M. Y. (2010). Use of polymerase chain reaction in the diagnosis of dermatophilosis from cattle, sheep and goats in Nigeria. J. Anim.Vet.Adv. 9: 1034-1036
    12. Wen-Xing, H., Yu, C., Jingmei, W., Yuvanzhi, W. and Gen-Quiang, Y. (2007). Establishment of polymerase chain reaction assay for detection of dermatophilosis in sheep. . http://www.paper.edu.cn/en/paper.serial number 200704-270
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