Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 4 (august 2015) : 512-514,   Doi: 10.5958/0976-0555.2015.00083.7

Diagnostic potential of polymerase chain reaction in detection of classical swine fever virus infection in slaughtered pigs

M
M. Rout
G
G. Saikumar
K
K. Nagarajan
1Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, India.
Cite article:- Rout M., Saikumar G., Nagarajan K. (2025). Diagnostic potential of polymerase chain reaction in detection of classical swine fever virus infection in slaughtered pigs. Indian Journal of Animal Research. 49(4): 512-514. doi: 10.5958/0976-0555.2015.00083.7.

ABSTRACT

The present work was carried out to study the diagnostic potential of reverse transcription polymerase chain reaction (RT-PCR) in detection of classical swine fever virus (CSFV) infection in slaughtered pigs of Bareilly, Uttar Pradesh during 2005. A total of 1120 pigs were examined for CSF suggestive pathological lesions and tissue samples were tested for CSFV genome by RT-PCR. Out of 1120 cases examined, 110 (9.82%) showed lesions suggestive for CSF. Based on pathological findings, 58.18% (64/110) were categorized as acute, 16.36% (18/110) as chronic and 25.45% (28/110) as inapparent form of CSF. Based on RT-PCR targeting 5’NTR and E2/NS2 region, the prevalence of CSF was found to be 4.82% (54/1120) and 3.83% (43/1120), respectively. RT-PCR thus ensures a sensitive and specific detection of CSFV and hence can be considered as a screening method of choice.

REFERENCES

  1. Benson, D.V. (1952). The value of inclusion bodies in the diagnosis of hog cholera. Am. J. Vet. Res., 13: 304-308.
  2. Gaede, W. (2002). Detection of classical swine fever with the Light Cycler instrument. Biochemica., 3: 4-6.
  3. Katz, J.B., Ridpath, J.F. and Bolin, S.R. (1993). Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested amplification approach. J. Clin. Microbiol., 3: 565-568.
  4. Mengeling, W.L. (1970). Endogenous neutralization of virus during fatal hog cholera illness. Am. J. Vet. Res., 31: 91-95.
  5. Meyers, G. and Thiel, H.J. (1996). Molecular characterization of pestiviruses. Adv. Virus. Res., 47: 53-118.
  6. Moennig, V., Floegel-Niesmann, G. and Greiser-Wilke, I. (2003). Clinical signs and epidemiology of classical swine fever: A review of new knowledge. Vet. J., 165: 11-20.
  7. Terpstra, C. and Wensvoort. G. (1988). Natural infections of pigs with bovine viral diarrhoea virus associated with signs resembling swine fever. Res. Vet Sci., 45: 137-114
  8. Urman, H.K., Underdahl, N.R., Aiken, J.M., Stair, E.L. and Young, G.A. (1962). Intranuclear inclusion bodies associated with hog cholera. J. Am. Vet. Med. Assoc., 141: 571-581.
  9. Van Oirschot, J.T. (1999). Hog Cholera/Classical swine fever. In: Disease of swine, 5th edn, (edited by B. E. Shaw) Iowa State University Press Ames, 289-300.
  10. Young, S.H. (1970). The use of supplemental tests in the diagnosis and eradication of hog cholera. J. Am. Vet. Med. Assoc., 157: 1855-1859.
  11. Zhang, H., Cao, H.W., Wu, Z.J. and Cui, Y.D. (2011a). A review of molecular characterization of Classical swine fever virus (CSFV). Israel J. Vet. Med. 66: 89-95.
  12. Zhang, H., Cao, H.W., Wu, Z.J. and Cui, Y.D. (2011b). Evolutionary rate of E2 genes of classical swine fever virus in China. Israel J. Vet. Med. 66: 161-163.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)