Correlation analysis between milk and serum LPBE FMD virus type specific antibody titres in buffaloes vaccinated with polyvalent oil adjuvant FMD vaccine

DOI: 10.5958/0976-0555.2015.00134.X    | Article Id: B-2681 | Page : 508-511
Citation :- Correlation analysis between milk and serum LPBE FMD virus type specific antibody titres in buffaloes vaccinated with polyvalent oil adjuvant FMD vaccine.Indian Journal of Animal Research.2015.(49):508-511
Vandna Bhanot, Ravindra Sharma, Anshu Sharma and Kamaldeep vandna.van@gmail.com
Address : Regional Research Centre on FMD, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar125 001, India.


The present investigation was carried out on 15 randomly selected milch buffaloes divided into three groups on the basis of lactation of an organized farm, to study the Foot and Mouth Disease Virus (FMDV) type specific antibodies in milk and serum following FMD vaccination. Milk and serum samples collected before vaccination i.e. 0 day and on 7, 14, 28, 42 and 56 days post vaccination, were analyzed for the detection of FMD virus type specific antibodies by liquid phase blocking ELISA (LPBE) . Milk LPBE antibody titres against different FMD virus types were significantly different showing higher antibody titres against FMD virus type O followed by A and Asia 1. Serum LPBE antibody titres against FMD virus type O and Asia 1 were not significantly different, except for type A. Milk and serum LPBE antibody titres were positively correlated with value of regression coefficient (R) as 0.6376 and correlation was highly significant (P


Foot and Mouth disease LPB-ELISA Milk and serum antibody Specific isotype antibody response.


  1. Anonymous (2002). Annual Report 2001-2002. Regional Research Center on FMD, CCSHAU, Hisar, India.
  2. Armstrong, R.M. (1997a). Development of tests for antibodies against FMD virus in cattle milk. J. Virol. Methods, 63:175-180.
  3. Armstrong, R.M. (1997b). The detection of antibodies against FMD virus in sheep milk. J. Virol. Methods, 69:45-51.
  4. Armstrong, R.M., Mathew, E.S. and Mackay, D.K.J. (2000). Validation of the specific isotype assay to detect antibodies against FMD virus in bovine milk. J. Virol. Methods, 85:193-201.
  5. Armstrong, R.M. and Mathew, E.S. (2001). Predicting herd protection against FMD by testing individual and bulk tank milk samples. J. Virol. Methods, 97:87-99.
  6. Dhennin, L., Labie, J. and Gicquel, B. (1997). Recherche sur les anticorps anti O aohteux contenus dans le lait de vaches vaccines. Bull. Acad. Vet. France, 52:513-521.
  7. Elevander, M., Edwards, S., Naslund, K. and Linde, N. (1995). Evaluation and application of an indirect ELISA for the detection of antibodies to bovine respiratory syncytial virus in milk, bulk milk and serum. J. Vet. Diag. Invest., 7:177-182.
  8. Golding, S.M., Hedger, R.S. and Talbot, P. (1976). Radial immuno-diffusion and serum neutralization techniques for the assay of antibodies to swine vesicular disease. Res. Vet. Sci., 20:142-147.
  9. Hamblin, C., Barnett, I.T.R. and Hedger, R.S. (1986). A new enzyme linked immunosorbent assay (ELISA) for detection of antibodies against foot and mouth disease virus. J. of Immunol. Methods., 93:115-121.
  10. Niskanen, R., Alenius, S., Larsson, B. and Jacobsson, S.O. (1991). Determination of level of antibodies to bovine viral diarrhea virus (BVDV) in bulk tanks as a pool in the diagnosis and prophylaxix of BVDV infection in dairy herds. Arch. Virol., 3:245-251.

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