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Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 4 (august 2015) : 434-437,   Doi: 10.5958/0976-0555.2015.00161.2

Phylogenetic analysis and comparative study of Sahiwal cattle CD14 gene

V
V. Kumar
I
I.D. Gupta
1Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India.
Cite article:- Kumar V., Gupta I.D. (2025). Phylogenetic analysis and comparative study of Sahiwal cattle CD14 gene . Indian Journal of Animal Research. 49(4): 434-437. doi: 10.5958/0976-0555.2015.00161.2.

ABSTRACT

Cluster of Differentiation-14 (CD14) gene is one of the strong candidate genes for mastitis resistance trait. Bovine CD14 gene spans over 2630 bp having 2 exons. Present investigation is based on phylogenetic analysis and comparative study of CD14 gene in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. Sequence analysis revealed that Sahiwal cattle CD14 gene sequence is 73 to 99% identical with several species. Phylogenetic analysis indicated that Sahiwal cattle (Bos indicus) and Bos taurus are genetically the most similar (99%) followed by Bubalus bubalis. The predicted peptide sequence revealed 373 amino acids precursor corresponding to coding sequence of CD14 gene of Sahiwal cattle. Amino acid length of CD14 protein was found different in reported species. BLAST analysis also revealed that Sahiwal cattle CD14 protein is 73 to 100% similar with other reported species. The sites for leucine-rich repeats, leucine rich nuclear export signal, putative N-linked glycosylation, O-linked glycosylation and disulphide bridges were predicted and comparative study was done.

REFERENCES

  1. Beutler, B. and Rietschel, E.T. (2003). Innate immune sensing and its roots: the story of endotoxin. Nat. Rev. Immunol.,
  2. 3: 169–176.
  3. Cour, T. L., Kiemer, L., Mølgaard, A., Gupta, R., Skriver, K. and Brunak, S. (2004). Analysis and prediction of leucine-rich nuclear export signals. Protein Engineering, Design and Selection, 17: 527–536.
  4. Ferrero, E., Hsieh, C. L., Francke, U. and Goyert, S.M. (1990). CD14 is amember of the family of leucine-rich proteins and is encoded by a gene syntenic with multiple receptor genes. Immunol., 145: 331–336.
  5. Gordon, S. (2002). Pattern recognition receptors: doubling up for the innate immune response. Cell, 111(7): 927–930.
  6. Ibeagha-Awemu, E.M., Lee, J.W., Ibeagha, A.E. (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils. BMC Genet., 9: 50-60.
  7. Kim, J. I., Lee, C. J., Jin, M. S., Lee, C. H., Paik, S. G., Lee, H. and Lee, J. O. 2005. Crystal structure of CD14 and its implication for lipopolysaccharide signaling. J. of Biol. Chem., 280(12): 11347-11351.
  8. Meng, J., Parroche, P., Golenbock, D.T. and McKnight, C.J. (2008). The differential impact of disulfide bonds and N-linked glycosylation on the stability and function of CD14. J. of Bio. Chem., vol. 283(6): 3376–3384.
  9. Ndungu, S.G., Ngumi, P.N., Mbogo, S.K., Dolan, T.T., Mutugi, J. J. and Young, A.S. (2005). Some preliminary observations on the susceptibility and resistance of different cattle breeds to Theileria parva infection. Onderstepoort Vet. Res., 72: 7–11.
  10. Ogorevc, J., Kunej, T., Razpet A. and Dovè, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet., 40: 832–851.
  11. Pal, A., Sharma, A., Bhattacharya, T.K., Chatterjee, P.N, and Chakravarty A.K. (2011). Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle. SAGE-Hindawi Access to Research, Molecular Biology International, vol. 2011, article ID 507346, 13 pages, doi: 10.4061/2011/507346.
  12. Sambrook, J. and Russell, D.W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
  13. Ulevitch, R.J. and Tobias, P.S. (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 13: 437-457.
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Indian Journal of Animal Research
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    volume 49 issue 4 (august 2015) : 434-437,   Doi: 10.5958/0976-0555.2015.00161.2

    Phylogenetic analysis and comparative study of Sahiwal cattle CD14 gene

    V
    V. Kumar
    I
    I.D. Gupta
    1Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India.
    Cite article:- Kumar V., Gupta I.D. (2025). Phylogenetic analysis and comparative study of Sahiwal cattle CD14 gene . Indian Journal of Animal Research. 49(4): 434-437. doi: 10.5958/0976-0555.2015.00161.2.

    ABSTRACT

    Cluster of Differentiation-14 (CD14) gene is one of the strong candidate genes for mastitis resistance trait. Bovine CD14 gene spans over 2630 bp having 2 exons. Present investigation is based on phylogenetic analysis and comparative study of CD14 gene in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. Sequence analysis revealed that Sahiwal cattle CD14 gene sequence is 73 to 99% identical with several species. Phylogenetic analysis indicated that Sahiwal cattle (Bos indicus) and Bos taurus are genetically the most similar (99%) followed by Bubalus bubalis. The predicted peptide sequence revealed 373 amino acids precursor corresponding to coding sequence of CD14 gene of Sahiwal cattle. Amino acid length of CD14 protein was found different in reported species. BLAST analysis also revealed that Sahiwal cattle CD14 protein is 73 to 100% similar with other reported species. The sites for leucine-rich repeats, leucine rich nuclear export signal, putative N-linked glycosylation, O-linked glycosylation and disulphide bridges were predicted and comparative study was done.

    REFERENCES

    1. Beutler, B. and Rietschel, E.T. (2003). Innate immune sensing and its roots: the story of endotoxin. Nat. Rev. Immunol.,
    2. 3: 169–176.
    3. Cour, T. L., Kiemer, L., Mølgaard, A., Gupta, R., Skriver, K. and Brunak, S. (2004). Analysis and prediction of leucine-rich nuclear export signals. Protein Engineering, Design and Selection, 17: 527–536.
    4. Ferrero, E., Hsieh, C. L., Francke, U. and Goyert, S.M. (1990). CD14 is amember of the family of leucine-rich proteins and is encoded by a gene syntenic with multiple receptor genes. Immunol., 145: 331–336.
    5. Gordon, S. (2002). Pattern recognition receptors: doubling up for the innate immune response. Cell, 111(7): 927–930.
    6. Ibeagha-Awemu, E.M., Lee, J.W., Ibeagha, A.E. (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils. BMC Genet., 9: 50-60.
    7. Kim, J. I., Lee, C. J., Jin, M. S., Lee, C. H., Paik, S. G., Lee, H. and Lee, J. O. 2005. Crystal structure of CD14 and its implication for lipopolysaccharide signaling. J. of Biol. Chem., 280(12): 11347-11351.
    8. Meng, J., Parroche, P., Golenbock, D.T. and McKnight, C.J. (2008). The differential impact of disulfide bonds and N-linked glycosylation on the stability and function of CD14. J. of Bio. Chem., vol. 283(6): 3376–3384.
    9. Ndungu, S.G., Ngumi, P.N., Mbogo, S.K., Dolan, T.T., Mutugi, J. J. and Young, A.S. (2005). Some preliminary observations on the susceptibility and resistance of different cattle breeds to Theileria parva infection. Onderstepoort Vet. Res., 72: 7–11.
    10. Ogorevc, J., Kunej, T., Razpet A. and Dovè, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet., 40: 832–851.
    11. Pal, A., Sharma, A., Bhattacharya, T.K., Chatterjee, P.N, and Chakravarty A.K. (2011). Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle. SAGE-Hindawi Access to Research, Molecular Biology International, vol. 2011, article ID 507346, 13 pages, doi: 10.4061/2011/507346.
    12. Sambrook, J. and Russell, D.W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
    13. Ulevitch, R.J. and Tobias, P.S. (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 13: 437-457.
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      Indian Journal of Animal Research

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