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Current IssueEditorial BoardOnline First ArticlesArchive

volume 48 issue 5 (october 2014) : 408-412,   Doi: 10.5958/0976-0555.2014.00002.8

NUCLEOTIDE SEQUENCE POLYMORPHISM WITHIN EXON 8 OF HEAT SHOCK PROTEIN (HSP) 90AA1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN DEONI COWS

S
Shahid Ahmad Shergojry*
K
K.P. Ramesha
N
Nazir Ahmad Mir
O
Ovais Aarif
1National Dairy Research Institute, Southern Campus, Adugodi, Bangalore-560 030, India.
Cite article:- Shergojry* Ahmad Shahid, Ramesha K.P., Mir Ahmad Nazir, Aarif Ovais (2025). NUCLEOTIDE SEQUENCE POLYMORPHISM WITHIN EXON 8 OF HEAT SHOCK PROTEIN (HSP) 90AA1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN DEONI COWS. Indian Journal of Animal Research. 48(5): 408-412. doi: 10.5958/0976-0555.2014.00002.8.

ABSTRACT

A group of proteins known as Heat Shock Proteins (HSPs) are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. PCR-SSCP technique and direct sequencing was used to identify novel SNPs in Exon 8 of HSP90AA1 gene in Deoni cows.  In Exon 8, three unique SSCP patterns corresponding to TT, GG and TG genotype with frequencies of 0.250, 0.639 and 0.111, respectively were observed. The sequence analysis of Exon 8 revealed T ® G transversion at position 3650 of HSPAA1 gene (GenBank accession number NC-007319 as reference sequence). The observed polymorphism (T ® G) at position 3650 results in substitution of an amino acid from Phenylalanine to Leucine. The cows having TG genotype had significantly higher lactationMilk Yield (Kg) as compared to cows with TT and GG genotypes (P£0.01). There was no difference in Lactation Length (days) in cows with different genotypes.

KEYWORDS

    REFERENCES

    1. Chen, B., Zhong, D.and Monteiro, A. (2006). Comparative genomics and evolution of the HSP90 family of genes across all kingdoms of organisms. BMC Genomics, 7: 156.
    2. Ellis, R.J. (2006). Protein misassembly: macromolecular crowding and molecular chaperones. In: [Csermely P, Vígh L (eds)] Molecular Aspects of the Stress Response: Chaperones, Membranes and Networks. Springer Science plus Business Media, NY, USA,pp 2–13
    3. Hall, T.A. (1999). Bioedit: a user- friendly biological sequence alignment editor and anlaysis program for windows 95/98/ NT. Nucleic Acids Symp Ser 41: 95-98.
    4. Li, Q., Han, J., Du, F., Ju, Z., Huang, J., Wang, J., Li, R., Wang, C. and Zhong, J. (2011). Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Molecular Biology Report, 38: 2657–2663.
    5. Marcos-Carcavilla, Ane., Mutikainen, Mari., González, Carmen.,Jorge, H., Calvo, Juha., Kantanen, Albina., Sanz, Nurbiy, S., Marzanov-María, D., Pérez-Guzmán and Magdalena Serrano, J.B. (2010). A SNP in the HSP90AA1 gene 52 flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress and Chaperones. 15:67–81.
    6. Miller, S.A., Dykes, D. D. and Polesky, H .F. (1988). A sample salting out procedure for extracting DNA from humane nucleated cells. Nucleic Acids Res. 16: 1215.
    7. Reddacliff, L.A., Beh, K., McGregor, H. and Whittington,R.J. (2005). A preliminary study of possible genetic influences on the susceptibility of sheep to Johne’s disease.Aust. Vet J. 83:435–441.
    8. Sambrook, J. and Russel, D.W. (2001). Molecular Cloning A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, Newyork.
    9. SAS Institute, Inc. (2003), Release 9.2 (TS2MO) of the SAS® System for Microsoft®ndows®, SAS Institute, Inc., Cary, NC, USA.
    10. Snedecor G W and Cochran W G. (1994).Statistical Methods.8th ed. Oxford and IBH Publishing, Kolkata.
    11. Umaporn, Pastsart., Anuchai, Pinyopummintr., Jeerachai, janapruthipong. and Voravit, Siripholvat. (2006). Heat Shock protein 90 (hsp90) gene polymorphism associated with heat tolerance traits in crossbred dairy cattle and Thai native cattle. Agricultural Sci. J. 37 (5): 393-398.
    12. Yeh FR, Yang. Boyle T (1999) POPGENE version 1.31.Microsoft Windows-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research, Canada. Available at (http://www.ualberta.ca/ ~fyeh/ fyeh)
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    Indian Journal of Animal Research
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      volume 48 issue 5 (october 2014) : 408-412,   Doi: 10.5958/0976-0555.2014.00002.8

      NUCLEOTIDE SEQUENCE POLYMORPHISM WITHIN EXON 8 OF HEAT SHOCK PROTEIN (HSP) 90AA1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN DEONI COWS

      S
      Shahid Ahmad Shergojry*
      K
      K.P. Ramesha
      N
      Nazir Ahmad Mir
      O
      Ovais Aarif
      1National Dairy Research Institute, Southern Campus, Adugodi, Bangalore-560 030, India.
      Cite article:- Shergojry* Ahmad Shahid, Ramesha K.P., Mir Ahmad Nazir, Aarif Ovais (2025). NUCLEOTIDE SEQUENCE POLYMORPHISM WITHIN EXON 8 OF HEAT SHOCK PROTEIN (HSP) 90AA1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN DEONI COWS. Indian Journal of Animal Research. 48(5): 408-412. doi: 10.5958/0976-0555.2014.00002.8.

      ABSTRACT

      A group of proteins known as Heat Shock Proteins (HSPs) are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. PCR-SSCP technique and direct sequencing was used to identify novel SNPs in Exon 8 of HSP90AA1 gene in Deoni cows.  In Exon 8, three unique SSCP patterns corresponding to TT, GG and TG genotype with frequencies of 0.250, 0.639 and 0.111, respectively were observed. The sequence analysis of Exon 8 revealed T ® G transversion at position 3650 of HSPAA1 gene (GenBank accession number NC-007319 as reference sequence). The observed polymorphism (T ® G) at position 3650 results in substitution of an amino acid from Phenylalanine to Leucine. The cows having TG genotype had significantly higher lactationMilk Yield (Kg) as compared to cows with TT and GG genotypes (P£0.01). There was no difference in Lactation Length (days) in cows with different genotypes.

      KEYWORDS

        REFERENCES

        1. Chen, B., Zhong, D.and Monteiro, A. (2006). Comparative genomics and evolution of the HSP90 family of genes across all kingdoms of organisms. BMC Genomics, 7: 156.
        2. Ellis, R.J. (2006). Protein misassembly: macromolecular crowding and molecular chaperones. In: [Csermely P, Vígh L (eds)] Molecular Aspects of the Stress Response: Chaperones, Membranes and Networks. Springer Science plus Business Media, NY, USA,pp 2–13
        3. Hall, T.A. (1999). Bioedit: a user- friendly biological sequence alignment editor and anlaysis program for windows 95/98/ NT. Nucleic Acids Symp Ser 41: 95-98.
        4. Li, Q., Han, J., Du, F., Ju, Z., Huang, J., Wang, J., Li, R., Wang, C. and Zhong, J. (2011). Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Molecular Biology Report, 38: 2657–2663.
        5. Marcos-Carcavilla, Ane., Mutikainen, Mari., González, Carmen.,Jorge, H., Calvo, Juha., Kantanen, Albina., Sanz, Nurbiy, S., Marzanov-María, D., Pérez-Guzmán and Magdalena Serrano, J.B. (2010). A SNP in the HSP90AA1 gene 52 flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress and Chaperones. 15:67–81.
        6. Miller, S.A., Dykes, D. D. and Polesky, H .F. (1988). A sample salting out procedure for extracting DNA from humane nucleated cells. Nucleic Acids Res. 16: 1215.
        7. Reddacliff, L.A., Beh, K., McGregor, H. and Whittington,R.J. (2005). A preliminary study of possible genetic influences on the susceptibility of sheep to Johne’s disease.Aust. Vet J. 83:435–441.
        8. Sambrook, J. and Russel, D.W. (2001). Molecular Cloning A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, Newyork.
        9. SAS Institute, Inc. (2003), Release 9.2 (TS2MO) of the SAS® System for Microsoft®ndows®, SAS Institute, Inc., Cary, NC, USA.
        10. Snedecor G W and Cochran W G. (1994).Statistical Methods.8th ed. Oxford and IBH Publishing, Kolkata.
        11. Umaporn, Pastsart., Anuchai, Pinyopummintr., Jeerachai, janapruthipong. and Voravit, Siripholvat. (2006). Heat Shock protein 90 (hsp90) gene polymorphism associated with heat tolerance traits in crossbred dairy cattle and Thai native cattle. Agricultural Sci. J. 37 (5): 393-398.
        12. Yeh FR, Yang. Boyle T (1999) POPGENE version 1.31.Microsoft Windows-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research, Canada. Available at (http://www.ualberta.ca/ ~fyeh/ fyeh)
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