Genetic polymorphism of melatonin receptor 1A (MTNR1A) gene in Corriedale sheep of Kashmir, India

DOI: 10.5958/0976-0555.2015.00119.3    | Article Id: B-2661 | Page : 448-450
Citation :- Genetic polymorphism of melatonin receptor 1A (MTNR1A) gene in Corriedale sheep of Kashmir, India.Indian Journal Of Animal Research.2015.(49):448-450
Tavsief Ahmad, T.A.S. Ganai, Ankit Magotra, R.K. Sharma1 and Ovais Aarif
Address : Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, SKUAST-Kashmir,Shuhama(Alusteng) Srinagar, 190 006, India.


The aim of the present work was to explore and characterize the different polymorphic variants of exon II region of MTNR1A gene in 31Corriedale sheep of Kashmir. Genomic DNA was extracted and subjected to PCR for the amplification of the main part of exon II of the ovine MTNR1A gene. PCR products were subjected to restriction enzyme (RE) digestion by MnlI restriction enzyme and depending upon number of sites in the fragment genotypes were classed as MM, Mm and mm genotypes for MnlI RE. Representative samples of all identified alleles were sequenced from forward as well as reverse side. Allelic frequencies were 0.62 and0.38 for allele M and m respectively. The population was observed to be in Hardy-Weinberg equilibrium with regard to allele M and m. Results indicated the existence of polymorphism in the exon II of MTNR1A locus in the breed under study. The various mutational positions recorded in the MTNR1A locus were G169T, G328A, G422A, and C607T. The mutation G422A resulted in amino acid change from Valine to Isoleucine while all other mutations were silent.


Equilibrium Hardy-Weinberg MTNRIA gene MnlI PCR-RFLP Sequencing Sheep Seasonal reproduction.


  1. Barrett, P., Conway, S., Jockers, R., Strosberg, A.D., Guardiola-Lemaitre, B., Delagrange, P. and Morgan, P.J., (1997). Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor. Biochim. Biophys. Acta1356 : 299-307
  2. Carcangiu, V., Vacca, G.M., Mura, M.C., Dettori, M. L., Pazzola, M., Luridana, S. and Bini, P.P. (2009). Relationship between MTNR1A melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds. Animal Reproduction Science 110 : 71-78
  3. Chu, M. X., Ji, C.L. and Chen, G.H. (2003). Association between PCR-RFLP of melatonin receptor 1a gene and high prolificacy in Small Tail Han sheep. Asian-Aust. J. Anim. Sci. 16 :1701-1704.
  4. Ebisawa, T., Karne, S., Lerner, M.R. and Reppert, S.M. (1994). Expression cloning of a high-affinity melatonin receptor from Xenopusdermal melanophores.ProcNatlAcadSci 91 : 6133-6137
  5. Falconer, D.S. and Mackay, T.F.C. (1996). Introduction to Quantitative Genetics, 4th edition CABI New York.
  6. Mateescu, R.G., Lunsford, A.K. and Thonney, M.L. (2009). Association between melatonin receptor 1A gene polymorphism and reproductive performance in Dorset ewes.J. Animal Sci. 87 : 2485-2488
  7. Messer, L.A., Wang, L., Christopher, K., Tuggle, C.K., Yerle, M., Chardon, P., Pomp, D., Womack, J.E., Barendse, W., Crawford, A.M., Notter, D.R. and Rothschild, M.F. (1997). Maping of the melatonin receptor 1a (MTNR1a) gene in pigs, sheep and Cattle.Mamm. Genome 8 : 368-370.
  8. Migaud, M., Gavet, S. and Pelletier, J. (2002). Partial cloning and polymorphism of the melatonin 1a (Mel1a) receptor gene in two breeds of goat with different reproductive seasonality. Reproduction 124 : 59-64.
  9. Notter, D.R. (1992). Genetic improvement of out-of-season breeding through selection.Proc. Out-of-Season Breed.Symp. Iowa State Univ. Ames., pp. 55-81.
  10. Notter, D.R., Cockett, N.E. and Hadfield, T.S. (2003). Evaluation of melatonin receptor 1a as a candidate gene influencing reproduction in an autumn-lambing sheep flock.J. Anim. Sci. 81, 912–917.
  11. Pelletier, J., Bodin, L., Hanocq, E., Malpaux, B., Teyssier, J., Thimonier, J. and Chemineau, P. (2000). Association between expression of reproductive seasonality and alleles of the gene Mel1a receptor in the ewe.Biol. Reprod. 62 : 1096-1101.
  12. Reppert, S.M., Weaver, D.R. and Ebisawa, T. (1994). Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 13 : 1177-1185
  13. Sambrook, J. and Russel, D.W. (2001). Molecular cloning, a laboratory manual.3rd edition.Cold Spring Harbour Laboratory Press, New York USA.
  14. Thiery, J.C., Chemineau, P., Hernandez, X., Migaud, M. and Malpaux, B. (2002). Neuroendocrine interactions and seasonality.Domest. Anim. Endocrinol. 23 : 87-100.

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