Loading...

Indian Journal of Animal Research

Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen

DOI: 10.5958/0976-0555.2015.00091.6    | Article Id: B-2660 | Page : 470-473
Citation :- Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen.Indian Journal of Animal Research.2015.(49):470-473
P. Perumal and C. Rajkhowa perumalponraj@gmail.com
Address : ICAR-National Research Centre on Mithun, Jharnapani, Nagaland – 797 106, India.

Abstract

A total 50 ejaculates from eight mithun bulls were collected twice a week over 8 months and semen pooled to eliminate individual differences and was split into four equal aliquots, diluted with the TEYC extender. Group 1: diluted 1:2 with TEYC extender (control), group 2 to 4: semen diluted with 1:2 TEYC extender supplemented with 6, 8 and 10 ml of PJ/ 100 ml of diluent, respectively. The seminal parameters and biochemical profiles were assessed at 5oC for 0, 6, 12, 24 and 30 h of incubation. Inclusion of PJ into diluent resulted in significant (p

Keywords

Antioxidants Mithun Pomegranate (Punica granatum) juice Seminal parameters.

References

  1. Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D. and Fuhrman, B. (2000). Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apoliprotein E- deficient mice. Am. J. Clin. Nutr.71: 1062 - 1076.
  2. Cohen, G.D., Demblec, K. and Marcus, J. (1970). Measurement of catalase activity in tissue extracts. Anal. Biochem. 34: 30 - 38.
  3. Kaplan, M., Hayek, T., Raz, A., Coleman, R., Dornfeld, L., Vaya, J. and Aviram, M. (2001). Pomegranate juice supplementation to atherosclerotic mice reduces macrophage lipid peroxidation, cellular cholesterol accumulation and development of atherosclerosis. J. Nutr. 131: 2082 – 2089.
  4. Lansky, E., Shubert, S. and Neman, I. (2002). Pharmacological and therapeutic properties of pomegranate. CIHEAM – Options Mediterraneennes. 2: 231 – 235
  5. Madesh, M. and Balasubramanian, A.K. (1997). Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochem. Biophysics. 35: 184 -188.
  6. Perumal, P., Selvaraju, S., Barik, A.K., Mohanty, D.N., Das, S. and Mishra, P.C. (2011b). Role of reduced glutathione in improving post-thawed frozen seminal characters of poor freezable Jersey crossbred bull semen. Indian J. Anim. Sci. 81(8): 807-810.
  7. Perumal, P., Selvaraju, S., Selvakumar, S., Barik, A.K., Mohanty, D.N., Das, S., Das, R.K. and Mishra, P.C. (2011a). Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred jersey bulls on sperm parameters and conception rates. Reprod. Dom. Anim. 46(4): 636-641.

Global Footprints