Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 49 issue 4 (august 2015) : 470-473

Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen

P. Perumal, C. Rajkhowa
1ICAR-National Research Centre on Mithun, Jharnapani, Nagaland – 797 106, India.
Cite article:- Perumal P., Rajkhowa C. (2024). Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen. Indian Journal of Animal Research. 49(4): 470-473. doi: 10.5958/0976-0555.2015.00091.6.

ABSTRACT

A total 50 ejaculates from eight mithun bulls were collected twice a week over 8 months and semen pooled to eliminate individual differences and was split into four equal aliquots, diluted with the TEYC extender. Group 1: diluted 1:2 with TEYC extender (control), group 2 to 4: semen diluted with 1:2 TEYC extender supplemented with 6, 8 and 10 ml of PJ/ 100 ml of diluent, respectively. The seminal parameters and biochemical profiles were assessed at 5oC for 0, 6, 12, 24 and 30 h of incubation. Inclusion of PJ into diluent resulted in significant (p

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