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volume 49 issue 4 (august 2015) : 503-507,   Doi: 10.5958/0976-0555.2015.00086.2

Comparison of indirect hemagglutination assay (iha) and coprological diagnosis of schistosomosis in cows and buffaloes

S
Sadaf Niaz
T
Tanweer Akhtar
N
Naser M. AbdEl-Salam
S
Sumaira Shams
S
Sultan Ayaz
R
Riaz Ullah
1College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan.
Cite article:- Niaz Sadaf, Akhtar Tanweer, AbdEl-Salam M. Naser, Shams Sumaira, Ayaz Sultan, Ullah Riaz (2025). Comparison of indirect hemagglutination assay (iha) and coprological diagnosis of schistosomosis in cows and buffaloes . Indian Journal of Animal Research. 49(4): 503-507. doi: 10.5958/0976-0555.2015.00086.2.

ABSTRACT

Schistosomosis is a disease of veterinary and medical importance caused by Schistosoma spp is characterized by frequent diarrhea with blood and mucous, weight loss and weakness in animals and causes significant morbidity, mortality and economic loss in cattle population. The study was designed to analyze/detect the schistosoma spp by coprological and serological examination (IHA) in cows and buffaloes of different areas of Punjab, Pakistan (Lahore, Sheikhupura, Gujranwala and Sialkot) from March 2007- February 2008. Both the techniques (coprological and serological examination (IHA) for Schistosomosis in cows and buffaloes were compared. Overall prevelance of Schistosomosis in cows and buffaloes was noted 14.0% and 13.6% by coprological examination and 17% and 16.3% by indirect hemagglutination assay (IHA), respectively. Statistical analysis showed non-significant difference in indirect hemagglutination assay (IHA) and fecal examination. Similarly month, area, age and sex wise seroprevalence of Schistosomosis was noted higher by IHA test than coprological examination in cows and buffaloes. Sensitivity (%) and specificity (%) of IHA test was noted 100% and >97.0%, respectively. The study reveals that Schistosomosis is prevalent in the study areas and needed proper preventive measure to minimize its prevalence. Besides that it is found that IHA is a good diagnostic tool for detection of Schistosomosis.

REFERENCES

  1. Al-Mofarreh, M., Ahmad, A., Hasan, S. and Al-Mofleh, I. (2000). “Formalether concentration method in the diagnosis of active schistosoma in patient with detectable IHA”. Saudi J. Gastroenterol., 6: 157-160.
  2. Azab, M.E. and El-Zayat, E.A. (1996). Evaluation of purified antigens in haemagglutination test (IHA) for determination of cross reactivities in diagnosis of fasciolosis and Schistosomosis. J. Egypt Soc. Parasitol., 26: 677-685.
  3. El-Ganayni, G.A. and Youssef, M.E. (1992). Evaluation of adult schistosoma and cercarial antigens in serodiagnosis of Schistosomosis using IHAT and ELISA. J. Egypt Soc. Parasitol., 22: 555-560.
  4. Katz, N., Chaves, A. and Pellegrino, J. (1972). A simple device for quantitative stool thicksmear technique in Schistosomosis. Rev. Inst. Med. Trop. Sao. Paulo., 14: 397-400.
  5. Makinson, A., Morales, R. J., Basset, D., Bouchaud, O., Verdon, R., Hosseini, H., Moing, V. L., Delaporte, E. and Reynes, J. (2008). Diagnostic approaches to imported schistosomal myeloradiculopathy in travelers. Neurology 71: 66-67.
  6. Pardo, J., Carranza, C., Turrients, M.C., Arellano, J.L.P., Velez, R.L., Ramajo, V. and Muro, A. (2004). Utility of S. bovis adult worm antigen for diagnosis of human Schistosomosis by ELISA and Electroimmunotransfer blot techniques. Clin. Diag. Lab. Immunol., 11: 1165-1169.
  7. Reilly, O.M.M. (1995). Tuberculin shin tests sensitivity and specificity in Mycobacterium bovis infection in animals and humans, 1st ed. Iowa State University Press/Ames, pp. 86-87.
  8. Sorgho, H., Bahgat, M., Poda, J.N., Song, W., Kirsten, C., Doenhoff, M.J. and Ruppel, A. (2005). Serodiagnosis of Schistosomosis in an endemic area of Burkina Faso. Performance of several immunological test with parasite antigens. Acta Trop., 93: 169-180.
  9. Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and Jennings, F.W. (2005). Veterinary Parasitology, ed. ELBS Langman, U.K.
  10. Van, Dijk, K., Starink, M.V., Bart, A., Nijhuis, E.W.P., van der Wal, A.C., van Thiel, P. P. A. M., de Vries, H. J. C. and van Gool, T. (2010). The Potential of Molecular Diagnosis of Cutaneous Ectopic Schistosomosis. Am J Trop Med Hyg 83: 958-959.
  11. Van, G.T., Vetter, H., Vervoort, T., Doenhoff, M.J., Wetsteyn, J. and Overbosch, D. (2002). Serodiagnosis of imported Schistosomosis by a combination of commercial indirect hemagglutination test with adult worm antigens and an enzyme-linked immunosorbent assay with schistosoma egg antigen. J. Clin. Microbiol., 40: 3432-3437.
  12. WHO (1994). “The control of Schistosomosis report of a WHO expert communities”. World Health Organization Technical Report Series.
  13. Xiang, X., Tianping, W. and Zhigang, T. (2003). Development of a rapid sensitive dye immunoassay for Schistosomosis diagnosis. J. Immunol. Method, 280: 49-57.
  14. Yamaguti, S. (1975). A synoptical review of life historics of digenetic trematode of vertebrate with special reference to the morphology of their larvae. Keiguka Publ. Co. Tokyo, pp. 410-413.
  15. Zhou, Y.B., Yang, M.X., Wang, Q.Z., Zhao, G.M., Wei, J.G. and Jiang, Q.W. (2007). Field comparison of immunodiagnostic and parasitological techniques for the detection of Schistosoma japonicum in the people’s Republic of China. Am. J. Trop. Med. Hyg., 76: 1138-1143.
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Indian Journal of Animal Research
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    volume 49 issue 4 (august 2015) : 503-507,   Doi: 10.5958/0976-0555.2015.00086.2

    Comparison of indirect hemagglutination assay (iha) and coprological diagnosis of schistosomosis in cows and buffaloes

    S
    Sadaf Niaz
    T
    Tanweer Akhtar
    N
    Naser M. AbdEl-Salam
    S
    Sumaira Shams
    S
    Sultan Ayaz
    R
    Riaz Ullah
    1College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan.
    Cite article:- Niaz Sadaf, Akhtar Tanweer, AbdEl-Salam M. Naser, Shams Sumaira, Ayaz Sultan, Ullah Riaz (2025). Comparison of indirect hemagglutination assay (iha) and coprological diagnosis of schistosomosis in cows and buffaloes . Indian Journal of Animal Research. 49(4): 503-507. doi: 10.5958/0976-0555.2015.00086.2.

    ABSTRACT

    Schistosomosis is a disease of veterinary and medical importance caused by Schistosoma spp is characterized by frequent diarrhea with blood and mucous, weight loss and weakness in animals and causes significant morbidity, mortality and economic loss in cattle population. The study was designed to analyze/detect the schistosoma spp by coprological and serological examination (IHA) in cows and buffaloes of different areas of Punjab, Pakistan (Lahore, Sheikhupura, Gujranwala and Sialkot) from March 2007- February 2008. Both the techniques (coprological and serological examination (IHA) for Schistosomosis in cows and buffaloes were compared. Overall prevelance of Schistosomosis in cows and buffaloes was noted 14.0% and 13.6% by coprological examination and 17% and 16.3% by indirect hemagglutination assay (IHA), respectively. Statistical analysis showed non-significant difference in indirect hemagglutination assay (IHA) and fecal examination. Similarly month, area, age and sex wise seroprevalence of Schistosomosis was noted higher by IHA test than coprological examination in cows and buffaloes. Sensitivity (%) and specificity (%) of IHA test was noted 100% and >97.0%, respectively. The study reveals that Schistosomosis is prevalent in the study areas and needed proper preventive measure to minimize its prevalence. Besides that it is found that IHA is a good diagnostic tool for detection of Schistosomosis.

    REFERENCES

    1. Al-Mofarreh, M., Ahmad, A., Hasan, S. and Al-Mofleh, I. (2000). “Formalether concentration method in the diagnosis of active schistosoma in patient with detectable IHA”. Saudi J. Gastroenterol., 6: 157-160.
    2. Azab, M.E. and El-Zayat, E.A. (1996). Evaluation of purified antigens in haemagglutination test (IHA) for determination of cross reactivities in diagnosis of fasciolosis and Schistosomosis. J. Egypt Soc. Parasitol., 26: 677-685.
    3. El-Ganayni, G.A. and Youssef, M.E. (1992). Evaluation of adult schistosoma and cercarial antigens in serodiagnosis of Schistosomosis using IHAT and ELISA. J. Egypt Soc. Parasitol., 22: 555-560.
    4. Katz, N., Chaves, A. and Pellegrino, J. (1972). A simple device for quantitative stool thicksmear technique in Schistosomosis. Rev. Inst. Med. Trop. Sao. Paulo., 14: 397-400.
    5. Makinson, A., Morales, R. J., Basset, D., Bouchaud, O., Verdon, R., Hosseini, H., Moing, V. L., Delaporte, E. and Reynes, J. (2008). Diagnostic approaches to imported schistosomal myeloradiculopathy in travelers. Neurology 71: 66-67.
    6. Pardo, J., Carranza, C., Turrients, M.C., Arellano, J.L.P., Velez, R.L., Ramajo, V. and Muro, A. (2004). Utility of S. bovis adult worm antigen for diagnosis of human Schistosomosis by ELISA and Electroimmunotransfer blot techniques. Clin. Diag. Lab. Immunol., 11: 1165-1169.
    7. Reilly, O.M.M. (1995). Tuberculin shin tests sensitivity and specificity in Mycobacterium bovis infection in animals and humans, 1st ed. Iowa State University Press/Ames, pp. 86-87.
    8. Sorgho, H., Bahgat, M., Poda, J.N., Song, W., Kirsten, C., Doenhoff, M.J. and Ruppel, A. (2005). Serodiagnosis of Schistosomosis in an endemic area of Burkina Faso. Performance of several immunological test with parasite antigens. Acta Trop., 93: 169-180.
    9. Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and Jennings, F.W. (2005). Veterinary Parasitology, ed. ELBS Langman, U.K.
    10. Van, Dijk, K., Starink, M.V., Bart, A., Nijhuis, E.W.P., van der Wal, A.C., van Thiel, P. P. A. M., de Vries, H. J. C. and van Gool, T. (2010). The Potential of Molecular Diagnosis of Cutaneous Ectopic Schistosomosis. Am J Trop Med Hyg 83: 958-959.
    11. Van, G.T., Vetter, H., Vervoort, T., Doenhoff, M.J., Wetsteyn, J. and Overbosch, D. (2002). Serodiagnosis of imported Schistosomosis by a combination of commercial indirect hemagglutination test with adult worm antigens and an enzyme-linked immunosorbent assay with schistosoma egg antigen. J. Clin. Microbiol., 40: 3432-3437.
    12. WHO (1994). “The control of Schistosomosis report of a WHO expert communities”. World Health Organization Technical Report Series.
    13. Xiang, X., Tianping, W. and Zhigang, T. (2003). Development of a rapid sensitive dye immunoassay for Schistosomosis diagnosis. J. Immunol. Method, 280: 49-57.
    14. Yamaguti, S. (1975). A synoptical review of life historics of digenetic trematode of vertebrate with special reference to the morphology of their larvae. Keiguka Publ. Co. Tokyo, pp. 410-413.
    15. Zhou, Y.B., Yang, M.X., Wang, Q.Z., Zhao, G.M., Wei, J.G. and Jiang, Q.W. (2007). Field comparison of immunodiagnostic and parasitological techniques for the detection of Schistosoma japonicum in the people’s Republic of China. Am. J. Trop. Med. Hyg., 76: 1138-1143.
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