Potential of methanolic extract of leaves of Hamiltonia suaveolens Roxb as the wound healer in rat

DOI: 10.5958/0976-0555.2015.00162.4    | Article Id: B-2626 | Page : 491-497
Citation :- Potential of methanolic extract of leaves of Hamiltonia suaveolens Roxb as the wound healer in rat.Indian Journal Of Animal Research.2015.(49):491-497
Manoj Kumar Chopda, Namrata Mahajan, Jaydeep Bhat , Mahesh Bhirud and Raghunath Mahajan mzczoo@yahoo.co.in
Address : Post Graduate Department of Zoology, Animal Physiology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425 002, India.

Abstract

Wound healing activity of methanolic extract of leaves of Hamiltonia suaveolens Roxb was assessed by using excision, incision and dead space wound model in Wistar rat. The animals were divided into five groups (n=6) and labeled as control; placebo; standard and experimental groups. Ointment of the methanolic extract at a dose 2.5% and 5% w/w were prepared and applied topically. The healing of the wound was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, biochemical profile and histopathology of granuloma tissue and Wound Image Analysis Software – WITA used for the first time. Animals were treated with the methanolic extract at a dose 5% exhibited 98.04% reduction in wound area 20th day, compared to control (93.82%). In incision wound model, tensile strength of healing tissue after treatment was found to be significantly higher compared to control group. The hydroxyproline (108.7 mg/g of tissue), ascorbic acid (50.69 mg/g of tissue) and glucosamine (33.22 mg/g of tissue) content from the wound area found significantly increased in treated animals at 5% concentration of methanolic extract as compared to control group of animals. Histological analysis revealed swift restoration of wound healing.

Keywords

Hamiltonia suaveolens Roxb Histology Hydroxyproline Image analysis software Skin breaking strength Wound contraction.

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