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GENETIC POLYMORPHISM OF BOOROLA FecB GENE AND ITS ASSOCIATION WITH LITTER SIZE IN BALANGIR, SHAHABADI AND BONPALA SHEEP BREEDS

DOI: 10.5958/0976–0555.2014.00448.8    | Article Id: B-2582 | Page : 307-314
Citation :- GENETIC POLYMORPHISM OF BOOROLA FecB GENE AND ITS ASSOCIATION WITH LITTER SIZE IN BALANGIR, SHAHABADI AND BONPALA SHEEP BREEDS.Indian Journal of Animal Research.2014.(48):307-314
Jowel Debnath* and Ran Vir Singh jowelivri@gmail.com
Address : Division of Animal Genetics and Breeding Indian Veterinary Research Institute, Izatnagar-243 122, India

Abstract

The aim of this study was to investigate the presence of Boorola FecB mutation gene in 250 selected ewes from different sheep flocks representing Balangir(100), Shahabadi(100) and Bonpala (50) by forced PCR-RFLP method. Genomic DNA was extracted from the blood of Balangir, Shahabadi and Bonpala matured ewes with average litter size varying from 1.00±0.00 to 1.183±0.04 at different parities . Digestion of  FecB gene  with AvaII restriction enzyme resulted FecB++ in all samples of Balangir  and Bonpala but in Shahabadi 76(76%) were FecBB+ and 24(24%) were FecB++. Sequencing of representative samples of  FecB  gene from each breed showed absence of point mutation i.e., A to G at 110th  position.  The overall average of litter size in Balangir, Shahabadi and Bonpala was 1.00±0.00, 1.41±0.02 and 1.00±0.00, respectively. Thus, the association between litter size and FecB++ genotype among Balangir and Bonpala sheep breeds were in unity. Analysis of variance indicated that the effect of genotype on litter size was highly significant.  FecB gene might have introgressed in Shahabadi sheep breed from Garole but the FecB gene has not been fixed in this breed. It needs further introgression of FecB gene through marker assisted introgression , so that each individual animal will lamb twins in future.

Keywords

Fecundity gene Forced PCR-RFLP Sheep breeds.

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