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volume 49 issue 1 (february 2015) : 8-13,   Doi: 10.5958/0976-0555.2015.00002.3

Screening of dairy breeding bulls for chromosomal profile and its andrological attributes

C
C. Jongmatoshi Jamir
C
C.S. Mukhopadhyay*
J
Jaspreet Singh Arora
G
G.S. Brah
R
Ranjana Cheema
M
Manmeet Kaur
1Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
Cite article:- Jamir Jongmatoshi C., Mukhopadhyay* C.S., Arora Singh Jaspreet, Brah G.S., Cheema Ranjana, Kaur Manmeet (2025). Screening of dairy breeding bulls for chromosomal profile and its andrological attributes. Indian Journal of Animal Research. 49(1): 8-13. doi: 10.5958/0976-0555.2015.00002.3.

ABSTRACT

Reproductive efficiency is a critical component and the most crucial issue faced by the dairy industries in India and abroad. The present study aimed at assessing the occurrences of male spermatological anomalies leading to poor reproductive performance and to study the chromosomal profile (G-banding) in dairy breeding bulls. Hypo-osmotic swelling test (HOST) and in vitro acrosome reaction were studied using fresh ejaculates of dairy breeding bulls. The metaphase plates did not reveal any chromosomal anomaly (either structural or numerical) in both the species.HOST results indicated significant differences between categories (high and low post thaw motility) but the scores did not differ significantly between the species. In vitro acrosome reaction showed that the bovine species showed significant difference (p

REFERENCES

  1. Ahmad, Javed K and Sattar A. (2004). Screening of breeding bulls of different breeds through karyotyping. Pak. Vet. J. 24: 190-92.
  2. Alveraz J G and Storey B T. (1993). Evidence that membrane stress contributes more than lipid peroxidation to sublethalcryodamage in cryopreserved human sperm : glycerol and other polyols as sole cryoprotectants. J.Andro. 14 : 199 - 08.
  3. Anonymous (2014) Sallient teatures of 19th livestock census. Press information bureau, Govt. of India New Delhi, pp I. URl; http:/pib.nic.in/newsite/print release aspxrelid=109280.
  4. Ax R L, Dickson K and Lenz R W. (1985). Induction of acrosome reactions by chondroitin sulphatein vitro corresponds to non return rates of dairy bulls. Journal of Dairy Science.68: 387-90.
  5. Bernt W D, Stark K H and Baguhl F. (1989). Diagnostic value of the hypo-osmotic swelling test in pathologic ejaculation findings.Zentralbl Gynaekol.111: 1604-09.
  6. Breininger E, Cetica P D and Beconi M T. (2010). Capacitation inducers act through diverse intracellular mechanisms in cryopreserved bovine sperm. Theriogenology.74: 1036–49
  7. Correa J R, Pace M M and Zavos P M. (1997). Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program. Theriogenology.48: 721–31.
  8. Critszer J K,Amerson B W and Asker D V. (1987).Cryopreservation of human spermatozoa. Post thaw chronology and zona free hamster ova penetration. Fertility and Sterility. 47: 980-84.
  9. De Castro M, Jayendran R S and Zaneveld L J. (1990). Hypo osmotic swelling test: analysis of prevasectomy ejaculates. Archives of Andrology. 24: 11-16.
  10. Ducos A, Revay T, Kovàcs A, HIdas A, Pinton A, Bonnet-Garnier A, Molteni L, Slota E, Switonski M, Arruga M V, Van Haeringen W A, Nicolae I, Chaves R, Guedes-Pinto H, Andersson M and Lannuzzi L. (2008). Cytogenetic screening of livestock populations in Europe: an overview. Cytogenetic and Genome Research.120: 26-41.
  11. Fechheimer N S.(1972). Causal basis of chromosomal abnormalities.Journal of Reproductive Fertility.(suppl.).15: 79.
  12. Fonseca J F, Torres C AA, Maffili V V, Borges A M, Santos A D F, Rodrigues M T and Oliveira R F M. (2005). The hypo osmotic swelling test in fresh goat spermatozoa Animal Reproduction.2(2): 139-44.
  13. Gustavsson I. (1969).Cytogenetics, distribution and phenotypic effects of a translocation in Swedish cattle.Hereditas.63: 68.
  14. Jadhav A. (1998). In vitro Assessment of spermatozoa in relationship to fertility and cytogenetic profile of bulls. Ph.D. dissertation. National Dairy Research Institute (NDRI), Karnal, Haryana, India.
  15. Jeyendran R S, Van Der Ven H H, Perez-Pelaez M, Crabo B G and Zaneveld L J D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal Reproductive Fertility.70: 219-28.
  16. Kale M M, Manik R S and Tomer O S. (2000).In-vitro assessment of crossbred buck fertility.Indian Journal of Animal Science.70: 25-29.
  17. Krumrych W. (2009). Overview of the cytogenetic evaluation of young bulls bred in the North-Western part of Poland in 1999-2008. Bulletin of the Veterinary Institute in Pulawy.53: 669-72.
  18. McLaughlinK J, Davies L and Seamark R F.(1990).Reproductive Fertility and Development.2: 619-22.
  19. Melander Y. (1959). The mitotic chromosomes of some cavicorn animals.Hereditas.49: 649.
  20. Mukhopadhyay C S, Verma A, Joshi B K and Singh A. (2008a). Prediction of Fertility of Crossbred Bulls under Field Conditions using Cryopreserved Semen.Indian Journal of Dairy Science, 61(4): 277-278.
  21. Mukhopadhyay CS and Verma A. (2007). Prediction of fertility of frozen thawed bovine-semen using Hypo osmotic swelling test. Indian Journal of Animal Production and Management. 23(1-4): 77-81
  22. Mukhopadhyay CS, Verma A, Joshi B K, Singh A and Chakravarty AK. (2008b). In Vitro Acrosome Reaction: It’s Pertinence in Assessing Fertility of Cryopreserved Semen. Indian Journal of Animal Research. 42(3): 201-204
  23. Neild D, Chaves G, Flores M, Mora N, Beconi M and Agüero A. (1999). Hypo osmotic test in equine spermatozoa. Theriogenology.51: 721-27.
  24. Okada A, Inomata K, Matsuhashi M, Fujio K, Miura K, Shiina K and Shirai M. (1990). Correlation of hypo osmotic swelling test, semen score and zona free hamster egg penetration assay in humans.InternationalJournalof Andrology.13: 337.
  25. Parrish J J, Susko-Parrish J L and First N L. (1989). Capacitation of bovine sperm by heparin: inhibitory effect of glucose and role of intracellular pH. Biology of Reproduction.41: 683–99.
  26. Patel RK and Khoda VK 1998: Presence of Variant chromosome 3 in the infertile water buffaloes (Bubalus bubalis). Veterinary Review. 13: 25-27.
  27. Prasad J K, Kumar S, Mohan G, Shanker U, Agarwal S K. (1999). Hypo-osmotic swelling tests (HOST) and its response in fresh and freeze-thawed semen. Indian Journal Animal Science.69: 766-69.
  28. Revell S G and Mrode R A.(1994). An osmotic resistance test for bovine semen. Animal Reproduction Science.36:77-86.
  29. Sasaki M S and Makino S. (1962).Revised study of chromosomes of domestic cattle and horse.Journal of Heredity.53: 157.
  30. Seabright M. (1971). A rapidly banding techniques for human chromosomes. Lancet.2: 971.
  31. Van der Ven H H, Jayendran R S, Hasani S, Perez-Peleaz M, Crabo B G and Zaneveld L J D. (1986).Correlation between human sperm swelling in hypo-osmotic medium (hypo osmotic swelling test) and in vitrofertilization. Journal of Andrology. 7: 190-96.
  32. Vasquez J M, Martinez E A, Martinez P, Garcia-Artiga C and Roca J. (1997).Hypo osmotic swelling of boar spermatozoa compared to other methods for analyzing the sperm membrane. Theriogenology.47: 913-22.
  33. Whitfield C H and Parkinson T J. (1995).Assessment of fertilizing potential of frozen bovine spermatozoa by in vitro induction of acrosome reactions with calcium ionophore (A 23187).Theriogenology. 44: 413-22.
  34. Yadav B R, Balakrishnan C R and Tomer O S. (1984).Chromosomal screening of male cattle and buffaloes.Indian Journal of Animal Science.54(6): 519-23.
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Indian Journal of Animal Research
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    volume 49 issue 1 (february 2015) : 8-13,   Doi: 10.5958/0976-0555.2015.00002.3

    Screening of dairy breeding bulls for chromosomal profile and its andrological attributes

    C
    C. Jongmatoshi Jamir
    C
    C.S. Mukhopadhyay*
    J
    Jaspreet Singh Arora
    G
    G.S. Brah
    R
    Ranjana Cheema
    M
    Manmeet Kaur
    1Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
    Cite article:- Jamir Jongmatoshi C., Mukhopadhyay* C.S., Arora Singh Jaspreet, Brah G.S., Cheema Ranjana, Kaur Manmeet (2025). Screening of dairy breeding bulls for chromosomal profile and its andrological attributes. Indian Journal of Animal Research. 49(1): 8-13. doi: 10.5958/0976-0555.2015.00002.3.

    ABSTRACT

    Reproductive efficiency is a critical component and the most crucial issue faced by the dairy industries in India and abroad. The present study aimed at assessing the occurrences of male spermatological anomalies leading to poor reproductive performance and to study the chromosomal profile (G-banding) in dairy breeding bulls. Hypo-osmotic swelling test (HOST) and in vitro acrosome reaction were studied using fresh ejaculates of dairy breeding bulls. The metaphase plates did not reveal any chromosomal anomaly (either structural or numerical) in both the species.HOST results indicated significant differences between categories (high and low post thaw motility) but the scores did not differ significantly between the species. In vitro acrosome reaction showed that the bovine species showed significant difference (p

    REFERENCES

    1. Ahmad, Javed K and Sattar A. (2004). Screening of breeding bulls of different breeds through karyotyping. Pak. Vet. J. 24: 190-92.
    2. Alveraz J G and Storey B T. (1993). Evidence that membrane stress contributes more than lipid peroxidation to sublethalcryodamage in cryopreserved human sperm : glycerol and other polyols as sole cryoprotectants. J.Andro. 14 : 199 - 08.
    3. Anonymous (2014) Sallient teatures of 19th livestock census. Press information bureau, Govt. of India New Delhi, pp I. URl; http:/pib.nic.in/newsite/print release aspxrelid=109280.
    4. Ax R L, Dickson K and Lenz R W. (1985). Induction of acrosome reactions by chondroitin sulphatein vitro corresponds to non return rates of dairy bulls. Journal of Dairy Science.68: 387-90.
    5. Bernt W D, Stark K H and Baguhl F. (1989). Diagnostic value of the hypo-osmotic swelling test in pathologic ejaculation findings.Zentralbl Gynaekol.111: 1604-09.
    6. Breininger E, Cetica P D and Beconi M T. (2010). Capacitation inducers act through diverse intracellular mechanisms in cryopreserved bovine sperm. Theriogenology.74: 1036–49
    7. Correa J R, Pace M M and Zavos P M. (1997). Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program. Theriogenology.48: 721–31.
    8. Critszer J K,Amerson B W and Asker D V. (1987).Cryopreservation of human spermatozoa. Post thaw chronology and zona free hamster ova penetration. Fertility and Sterility. 47: 980-84.
    9. De Castro M, Jayendran R S and Zaneveld L J. (1990). Hypo osmotic swelling test: analysis of prevasectomy ejaculates. Archives of Andrology. 24: 11-16.
    10. Ducos A, Revay T, Kovàcs A, HIdas A, Pinton A, Bonnet-Garnier A, Molteni L, Slota E, Switonski M, Arruga M V, Van Haeringen W A, Nicolae I, Chaves R, Guedes-Pinto H, Andersson M and Lannuzzi L. (2008). Cytogenetic screening of livestock populations in Europe: an overview. Cytogenetic and Genome Research.120: 26-41.
    11. Fechheimer N S.(1972). Causal basis of chromosomal abnormalities.Journal of Reproductive Fertility.(suppl.).15: 79.
    12. Fonseca J F, Torres C AA, Maffili V V, Borges A M, Santos A D F, Rodrigues M T and Oliveira R F M. (2005). The hypo osmotic swelling test in fresh goat spermatozoa Animal Reproduction.2(2): 139-44.
    13. Gustavsson I. (1969).Cytogenetics, distribution and phenotypic effects of a translocation in Swedish cattle.Hereditas.63: 68.
    14. Jadhav A. (1998). In vitro Assessment of spermatozoa in relationship to fertility and cytogenetic profile of bulls. Ph.D. dissertation. National Dairy Research Institute (NDRI), Karnal, Haryana, India.
    15. Jeyendran R S, Van Der Ven H H, Perez-Pelaez M, Crabo B G and Zaneveld L J D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal Reproductive Fertility.70: 219-28.
    16. Kale M M, Manik R S and Tomer O S. (2000).In-vitro assessment of crossbred buck fertility.Indian Journal of Animal Science.70: 25-29.
    17. Krumrych W. (2009). Overview of the cytogenetic evaluation of young bulls bred in the North-Western part of Poland in 1999-2008. Bulletin of the Veterinary Institute in Pulawy.53: 669-72.
    18. McLaughlinK J, Davies L and Seamark R F.(1990).Reproductive Fertility and Development.2: 619-22.
    19. Melander Y. (1959). The mitotic chromosomes of some cavicorn animals.Hereditas.49: 649.
    20. Mukhopadhyay C S, Verma A, Joshi B K and Singh A. (2008a). Prediction of Fertility of Crossbred Bulls under Field Conditions using Cryopreserved Semen.Indian Journal of Dairy Science, 61(4): 277-278.
    21. Mukhopadhyay CS and Verma A. (2007). Prediction of fertility of frozen thawed bovine-semen using Hypo osmotic swelling test. Indian Journal of Animal Production and Management. 23(1-4): 77-81
    22. Mukhopadhyay CS, Verma A, Joshi B K, Singh A and Chakravarty AK. (2008b). In Vitro Acrosome Reaction: It’s Pertinence in Assessing Fertility of Cryopreserved Semen. Indian Journal of Animal Research. 42(3): 201-204
    23. Neild D, Chaves G, Flores M, Mora N, Beconi M and Agüero A. (1999). Hypo osmotic test in equine spermatozoa. Theriogenology.51: 721-27.
    24. Okada A, Inomata K, Matsuhashi M, Fujio K, Miura K, Shiina K and Shirai M. (1990). Correlation of hypo osmotic swelling test, semen score and zona free hamster egg penetration assay in humans.InternationalJournalof Andrology.13: 337.
    25. Parrish J J, Susko-Parrish J L and First N L. (1989). Capacitation of bovine sperm by heparin: inhibitory effect of glucose and role of intracellular pH. Biology of Reproduction.41: 683–99.
    26. Patel RK and Khoda VK 1998: Presence of Variant chromosome 3 in the infertile water buffaloes (Bubalus bubalis). Veterinary Review. 13: 25-27.
    27. Prasad J K, Kumar S, Mohan G, Shanker U, Agarwal S K. (1999). Hypo-osmotic swelling tests (HOST) and its response in fresh and freeze-thawed semen. Indian Journal Animal Science.69: 766-69.
    28. Revell S G and Mrode R A.(1994). An osmotic resistance test for bovine semen. Animal Reproduction Science.36:77-86.
    29. Sasaki M S and Makino S. (1962).Revised study of chromosomes of domestic cattle and horse.Journal of Heredity.53: 157.
    30. Seabright M. (1971). A rapidly banding techniques for human chromosomes. Lancet.2: 971.
    31. Van der Ven H H, Jayendran R S, Hasani S, Perez-Peleaz M, Crabo B G and Zaneveld L J D. (1986).Correlation between human sperm swelling in hypo-osmotic medium (hypo osmotic swelling test) and in vitrofertilization. Journal of Andrology. 7: 190-96.
    32. Vasquez J M, Martinez E A, Martinez P, Garcia-Artiga C and Roca J. (1997).Hypo osmotic swelling of boar spermatozoa compared to other methods for analyzing the sperm membrane. Theriogenology.47: 913-22.
    33. Whitfield C H and Parkinson T J. (1995).Assessment of fertilizing potential of frozen bovine spermatozoa by in vitro induction of acrosome reactions with calcium ionophore (A 23187).Theriogenology. 44: 413-22.
    34. Yadav B R, Balakrishnan C R and Tomer O S. (1984).Chromosomal screening of male cattle and buffaloes.Indian Journal of Animal Science.54(6): 519-23.
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