Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 49 issue 6 (december 2015) : 764-769

The effects of in vitro maturation duration on maturation rate and intracytoplasmic sperm injection-derived embryonic development in goats

A.H. Nor Farizah, M.M. Rahman, W.E. Wan Khadijah, R.B. Abdullah*
1<p>Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences,&nbsp;Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.</p>
Cite article:- Farizah Nor A.H., Rahman M.M., Khadijah Wan W.E., Abdullah* R.B. (2015). The effects of in vitro maturation duration on maturation rate and intracytoplasmic sperm injection-derived embryonic development in goats . Indian Journal of Animal Research. 49(6): 764-769. doi: 10.18805/ijar.7036.

The aim of the present study was to evaluate the effects of in vitro maturation (IVM) duration on maturation rate and intracytoplasmic sperm injection (ICSI)-derived embryonic development in goat embryos. Donor goats were superovulated following oestrus synchronisation prior to laparoscopic oocyte pick-up. The quality of oocytes was scored based on cumulus cell layers, which were graded A, B and C. The oocytes were cultured in IVM medium with two different IVM durations (18-21 h and 22-25 h) for the ICSI procedure. A total of 327 matured oocytes were used, of which 157 and 170 oocytes were used for 18-21 h and 22-25 h IVM duration, respectively. The embryo cleavage rate of Grade A from the 18-21 h IVM group was significantly (P<0.05) higher than that of grades B and C. However, in the 22-25 h IVM duration group, the cleavage rates for all grades of oocytes were not significantly (P>0.05) different. Regardless of oocyte grades, no significant differences in maturation rates and cleavage rates for all stages of embryonic development between the two groups of IVM durations were observed. The results suggest that both IVM durations have the same potential in ICSI-derived embryonic development.

  1. Abdullah, R.B., Liow, S.L., Rahman, A.N.M.A., Chan, W.K., Wan Khadijah, W.E. and Ng, S.C. (2008). Prolonging the interval from ovarian hyperstimulation to laparoscopic ovum pick-up improved oocyte yield, quality, and developmental competence in goats. Theriogenology, 70: 765-771.

  2. Armstrong, D.T., Pfitzner, A.P., Warners, G.M., Ralph, M.M. and Seamark, R.F. (1983). Endocrine responses of goats to superovulation with PMSG and FSH. J. Reprod. Fertil., 67: 395-401. 

  3. Cognie, Y., Baril, G., Poulin, N. and Mermillod, P. (2003). Current status of embryo technologies in sheep and goat. Theriogenology, 59: 171-188.

  4. Combelles, C.M., Cekleniak, N.A., Racowsky, C. and Albertini, D.F. (2002). Assessment of nuclear and cytoplasmic maturation in in vitro matured human oocytes. Hum. Reprod., 17: 1006-1016.

  5. Dominko, T. and First N.L. (1997). Timing of meiotic progression in bovine oocytes and its effect on early embryo development. Mol. Reprod. Dev., 47: 456-467.

  6. Eichenlaub-Ritter, U., Schmiady, H., Kentenich, H. andSoewarto, D. (1995). Recurrent failure in polar body formation and premature chromosome condensation in oocytes from a human patient: indicators of asynchrony in nuclear and cytoplasmic maturation. Hum. Reprod., 10: 2343-2349.

  7. Evans, G. and Armstrong, D.T. (1983). Reproduction in fertilisation rate in vitro of oocytes from immature rats induced to superovulate. J. Reprod. Fertil., 70: 131-135.

  8. First, N.L., Leibfried-Rutledge, M.L. and Sirard, M.A. (1988). Cytoplasmic control of oocytes maturation and species differences in the development of maturational competence. In: Meiotic Inhibition: Molecular Control of Meiosis. Editors: F.P. Haseltine and N.L. First. National Institutes Health. New York. P.1.

  9. Inoue, A., Nakajima, R., Nagata, M. and Aoki, F. (2008). Contribution of the oocytes nucleus and cytoplasm to the determination of meiotic and developmental competence in mice. Hum. Reprod., 23: 1377-1384.

  10. Jimenez-Macedo, A.R., M.T. Paramio, B. Anguita, R. Morato, R. Romaguera, T. Mogas and D. Izquierdo. 2007. Effect of ICSI and embryo biopsy on embryo development and apoptosis according to oocyte diameter in prepubertal goats. Theriogenology, 67: 1399-1408.

  11. Kahraman, S., K. Yakin, E. Donmez, H. Samli, M. Bahce, G. cengiz, S. Sertyel, M. Samli and N. Imirzalioglu. 2000. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum. Reprod., 15: 2390-2393.

  12. Kato, Y. and Y. Nagao. 2009. Effect of PVP on sperm capacitation status and embryonic development in cattle. Theriogenology, 72: 624-635.

  13. Katska-Ksiazkiewicz, L., J. Opiela and B. Rynska. (2007). Effect of oocyte quality, semen donor and embryo co-culture system in the efficiency of blastocyst production in goats. Theriogenology, 62: 576-586.

  14. Kimura, Y. and Yanagimachi, R. (1995). Intracytoplasmic sperm injection in the mouse. Biol. Reprod., 52: 709-720.

  15. Kong, S.C. (2010). In vitro production of caprine embryos through intracytoplasmic sperm injection (ICSI) technique. MSc. Dissertation. University of Malaya, Kuala Lumpur, Malaysia.

  16. Krisher, R.L. (2004). The effect of oocyte quality on development. J. Anim. Sci., 82:14-23.

  17. Kubiak, J.Z. (1989). Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest. Dev. Biol., 136: 537-545.

  18. Moor, R.M., Osborn, J.C. and Crosby, I.M. (1985). Gonadotrophin-induced abnormalities in sheep oocytes after superovulation. J. Reprod. Fertil., 74: 167-172. 

  19. Nakagawa, K., Yamano, S., Moride, N., Yamashita, M., Yoshizawa, M. and Aono, T. (2001). Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertil. Steril., 76: 148-152.

  20. Nuti, L.C., Minhas, B.S., Baker, W.C., Capehart, J.S. and Marrack, P. (1987). Superovulation and recovery of zygotes from Nubian and Alpine dairy goats. Theriogenology, 28: 481-488.

  21. Palemo, G., Joris, H., Derde, M.P. and van Steirteghem, A.C. (1992). Pregnancies after intracytoplasmic injection of single spermatozoa into an oocyte. Lancet, 340: 17-18.

  22. Rahman, A.N.M.A., Abdullah, R.B. and Wan Khadijah, W.E. (2007). Goat embryo development from in vitro matured oocytes of heterogeneous quality through intracytoplasmic sperm injection technique. Biotechnology, 6: 373-382.

  23. Rahman, A.N.M.A. (2008). Goat embryo production from in vitro matured heterogeneous oocytes fertilised by intracytoplasmic sperm injection (ICSI) technique. PhD Thesis. University of Malaya, Kuala Lumpur, Malaysia.

  24. Rho, G.J., Hahnel,. A.C. and Betteridge, K.J. (2001). Comparisons if oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro. Theriogenology, 56: 503-516.

  25. Szczygiel, M.A. and Kurpisz, M. (2001). Chromosomal anomalies in human gametes and pre-implantation embryos, and their potential effect on reproduction. Andrologia, 33: 249-265.

  26. Tibary, A., Anouassian, A.A. and Khatir, H. (2005). Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology, 64: 618-638.

  27. Wang, B., Baldassarre, H., Pierson, J., Cote, F., Rao, K.M. and Karatzas, C.N. (2003). The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa. Zygote, 11: 219-227. 

  28. Williams, C.J. (2002). Signaling mechanisms of mammalian oocyte activation. Hum. Reprod. Update, 8: 313-321.

  29. Zheng, Y.L., Jiang, M.X., Zhang, Y.L., Sun, Q.Y. and Chen, D.Y. (2004). Effects of oocyte age, cumulus cells and injection methods on in vitro development of intracytoplasmic sperm injection rabbit embryos. Zygote, 12: 75-80.


Editorial Board

View all (0)