Association of TLR4 gene polymorphism with mastitis resistance and susceptibility in Frieswal cattle

DOI: 10.5958/0976-0555.2015.00010.2    | Article Id: B-2525 | Page : 48-50
Citation :- Association of TLR4 gene polymorphism with mastitis resistance and susceptibility in Frieswal cattle.Indian Journal of Animal Research.2015.(49):48-50
Rupal Shukla, Sulochana Sen, R.K. Ranjan and S.N.S. Parmar splsheckla@gmail.com
Address : Animal Genetics and Breeding, M.P.P.C.V.V. Jabalpur-482 001, M.P., India.


Toll-like receptors (TLRs) are essential in the host defense against microbial pathogens. Individual TLRs recognize distinct structural components of pathogens and evoke inflammatory responses.  In the present study, the amplicon of 316 bp of TLR4 gene was amplified in the thermal cycler (Gene Amp 6700, Applied Biosystem) using the primers of TLR4 gene and the genetic polymorphism was detected by Single Strand Conformation Ploymorphism (SSCP). The SSCP analysis of 316 bp region of  TLR4 gene revealed similar type of SSCP patterns in healthy, subclinical and clinical mastitis cases  suggesting that Frieswal cattle is monomorphic for TLR4 gene.


Frieswal Cattle Genetic polymer prism Mastites Toll-likereceptors (TLRs).


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