Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

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Indian Journal of Animal Research, volume 49 issue 3 (june 2015) : 292-294

Analysis of genetic polymorphism of IL8R receptor gene a marker associated with bovine mastitis among crossbred cattle

Sulochana Sen*, Rupal Shukla, Rakesh Ranjan, S.N.S. Parmar
1Animal Genetics and Breeding, M.P.P.C.V.V, Jabalpur-482 001, India.
Cite article:- Sen* Sulochana, Shukla Rupal, Ranjan Rakesh, Parmar S.N.S. (2025). Analysis of genetic polymorphism of IL8R receptor gene a marker associated with bovine mastitis among crossbred cattle . Indian Journal of Animal Research. 49(3): 292-294. doi: 10.5958/0976-0555.2015.00139.9.

ABSTRACT

A total of 60 crossbred cattle were screened for subclinical and clinical mastitis in and around Jabalpur using California mastitis test (CMT) followed by somatic cell count (SCC). The DNA was isolated and 311 bp fragments of IL8R gene was amplified using PCR. PCR-SSCP (Single Strand conformational polymorphism) method was used to identify genetic polymorphism of 311 bp amplicon of IL8R gene. PCR-SSCP revealed five SSCP patterns, SSCP pattern 1 (P1) was present in highest percentage 63.6% in healthy group, SSCP pattern 3 (P3) was present in highest percentage 64.2% in subclinical mastitis group while SSCP patterns (P2 and P5) were present in highest frequency 100% and 100% respectively in clinical mastitis group. Cattles exhibiting pattern P2, P5 and P3 were significantly (p

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