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Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 1 (february 2015) : 51-54,   Doi: 10.5958/0976-0555.2015.00011.4

Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam

M
M. Hussain*
P
P. Chakravarty
S
S. Soren
S
S. Deori
S
S. S. Begum
1National Research Centre on Yak, Dirang, West Kameng - 790 101, India.
Cite article:- Hussain* M., Chakravarty P., Soren S., Deori S., Begum S. S. (2025). Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam. Indian Journal of Animal Research. 49(1): 51-54. doi: 10.5958/0976-0555.2015.00011.4.

ABSTRACT

The study was undertaken to see the effect of recovery techniques and culture media on in-vitro maturation (IVM) of oocytes indigenous cattle of Assam. Oocytes were collected by aspiration and dissection techniques and were graded as A, B, C and D on the basis of their cumulus cell layers surrounding the zona pellucida. Oocytes with A, B and C categories were subjected to IVM using three different media (TCM-199, Ham’s F-12 and Ham’s F-10) with same additives (Sodium pyruvate, Gentamicin, L-glutamine, Porcine FSH, Follicular Fluid and HIOCS). Percentage of oocytes with expansion of cumulus cells was found to be the highest in dissection technique using TCM-199  media in comparison to Ham’s F-12 and Ham’s F-10 using same additives. However, it did not differ significantly between techniques. Incidence of Metaphase-I + Metaphase-II was the highest in oocytes recovered by dissection technique and cultured in TCM-199 with additives when incubated at 38.5°C for 24 hours and it differed significantly  between media but there was no significant difference between the recovery techniques. In conclusion, the culture medium TCM-199 along with additives may give better IVM rate of the oocytes recovered by dissection technique from indigenous cattle ovaries of Assam.

REFERENCES

  1. Alm, H., Torner, H., Kanitz, W. and Roschlau, K. (2008). Influence of oocytes recovery method, in-vitro fertilization method and serum source on embryonic development of in-vitro matured bovine oocytes. Archiv Tierzucht, Dummerstorf 51: 224–234.
  2. Barua, P M. (1995). Study on in-vitro oocytes maturation and in-vitro sperm capacitation in cattle. Ph.D. Thesis, submitted to Assam Agricultural University, Khanapara, Guwahati, Assam.
  3. Chauhan, M. S., Singla, S. K., Palta, P., Manik, R. S. and Madan, M. L. (1999). Effect of epidermal growth factor on the cumulus expansion, meiotic maturation and development of buffalo oocytes in-vitro. Vet. Rec., 144: 266–267.
  4. Garcia, J. M., Pinheiro, L. E. L., Almeida, I. L. Jr., Lima, V. F. R. De. and Mikich, A. B. (1988). Studies on in-vitro maturation of bovine oocytes in chemically defined media. Ars Veterinaria, 4 : 69–75
  5. Hafez, E.S.E. and Hafez, B. (2008). Reproduction in farm animals, seventh edition, (2008). 418, part VI Assisted Reproductive Technology.
  6. Hussain, M., Barua, P. M., Biswas, R. K., Dutta, D., Paul, V., Chakravarty, P., Deka, B. C. and Sarmah, B. C. (2012). In Vitro maturation of Indigenous cattle oocytes. The Indian Vet. J., 89: 59-62.
  7. Izadyar, F., Colenbrander, B. and Bevers, M. M. (1996). In-vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development. Mol. Reprod. Develop., 45: 372-377.
  8. Izadyar, F., Van-Tol, H. T., Colenbrander, B. and Bevers, M. M. (1997). Stimulatory effect of growth hormone on in-vitro maturation of bovine oocytes is exerted through cumulus cells and not mediated by IGF-I. Mol. Reprod. Develop., 47: 175-180.
  9. Lorenzo, P., Illera, M. J., Sanchez, J., Silvan, G., Illera, J. C. and Illera, M. (1993). Bovine oocytes maturation and fertilization in-vitro with growth factors and oestrus cow serum. Theriog., 39: 262.
  10. Martins, R. D., Costa, E. P., Chagas, J. S. C., Ignacio, F. S., Torres, C. A. A. and McManus, C. (2005). Effect of vitrification of immature bovine oocytes on in-vitro maturation. Anim. Reprod. Sci.,2: 128–134.
  11. Ocana-Quero, J. M., Pinedo Merlin, M., Ortega-Mariscal, M. and Moreno Millan, Y. M. (1999). The effect of different sera and bovine serum albumin fraction (BSA) on in-vitro maturation of immature bovine oocytes. Archivos De Zootecnia 48: 167-174.
  12. Rose, T. A. and Bavister, B. D. (1992). Effect of oocyte maturation medium on in-vitro development of in-vitro fertilized bovine embryos. Mol. Reprod. Develop., 31: 72-77.
  13. Shea, B. F., Latour, J. P.A., Bedirian, K. N. and Baker, R. D. (1976). Maturation in-vitro and subsequent penetrability of bovine follicular oocytes. J. Anim. Sci., 43: 809-815.
  14. Skinner, M. K. (1990). Mesenchymal (stromal) – epithelial cell interaction in the testis and ovary which regulate gonadal function. Reprod. Fert. Develop.,2: 237-244.
  15. Totey, S. M., Singh, G., Taneja, M., Pawshe, C. H. and Talwar, G. P. (1992). In-vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). J. Reprod. Fert., 95: 597-607.
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    volume 49 issue 1 (february 2015) : 51-54,   Doi: 10.5958/0976-0555.2015.00011.4

    Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam

    M
    M. Hussain*
    P
    P. Chakravarty
    S
    S. Soren
    S
    S. Deori
    S
    S. S. Begum
    1National Research Centre on Yak, Dirang, West Kameng - 790 101, India.
    Cite article:- Hussain* M., Chakravarty P., Soren S., Deori S., Begum S. S. (2025). Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam. Indian Journal of Animal Research. 49(1): 51-54. doi: 10.5958/0976-0555.2015.00011.4.

    ABSTRACT

    The study was undertaken to see the effect of recovery techniques and culture media on in-vitro maturation (IVM) of oocytes indigenous cattle of Assam. Oocytes were collected by aspiration and dissection techniques and were graded as A, B, C and D on the basis of their cumulus cell layers surrounding the zona pellucida. Oocytes with A, B and C categories were subjected to IVM using three different media (TCM-199, Ham’s F-12 and Ham’s F-10) with same additives (Sodium pyruvate, Gentamicin, L-glutamine, Porcine FSH, Follicular Fluid and HIOCS). Percentage of oocytes with expansion of cumulus cells was found to be the highest in dissection technique using TCM-199  media in comparison to Ham’s F-12 and Ham’s F-10 using same additives. However, it did not differ significantly between techniques. Incidence of Metaphase-I + Metaphase-II was the highest in oocytes recovered by dissection technique and cultured in TCM-199 with additives when incubated at 38.5°C for 24 hours and it differed significantly  between media but there was no significant difference between the recovery techniques. In conclusion, the culture medium TCM-199 along with additives may give better IVM rate of the oocytes recovered by dissection technique from indigenous cattle ovaries of Assam.

    REFERENCES

    1. Alm, H., Torner, H., Kanitz, W. and Roschlau, K. (2008). Influence of oocytes recovery method, in-vitro fertilization method and serum source on embryonic development of in-vitro matured bovine oocytes. Archiv Tierzucht, Dummerstorf 51: 224–234.
    2. Barua, P M. (1995). Study on in-vitro oocytes maturation and in-vitro sperm capacitation in cattle. Ph.D. Thesis, submitted to Assam Agricultural University, Khanapara, Guwahati, Assam.
    3. Chauhan, M. S., Singla, S. K., Palta, P., Manik, R. S. and Madan, M. L. (1999). Effect of epidermal growth factor on the cumulus expansion, meiotic maturation and development of buffalo oocytes in-vitro. Vet. Rec., 144: 266–267.
    4. Garcia, J. M., Pinheiro, L. E. L., Almeida, I. L. Jr., Lima, V. F. R. De. and Mikich, A. B. (1988). Studies on in-vitro maturation of bovine oocytes in chemically defined media. Ars Veterinaria, 4 : 69–75
    5. Hafez, E.S.E. and Hafez, B. (2008). Reproduction in farm animals, seventh edition, (2008). 418, part VI Assisted Reproductive Technology.
    6. Hussain, M., Barua, P. M., Biswas, R. K., Dutta, D., Paul, V., Chakravarty, P., Deka, B. C. and Sarmah, B. C. (2012). In Vitro maturation of Indigenous cattle oocytes. The Indian Vet. J., 89: 59-62.
    7. Izadyar, F., Colenbrander, B. and Bevers, M. M. (1996). In-vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development. Mol. Reprod. Develop., 45: 372-377.
    8. Izadyar, F., Van-Tol, H. T., Colenbrander, B. and Bevers, M. M. (1997). Stimulatory effect of growth hormone on in-vitro maturation of bovine oocytes is exerted through cumulus cells and not mediated by IGF-I. Mol. Reprod. Develop., 47: 175-180.
    9. Lorenzo, P., Illera, M. J., Sanchez, J., Silvan, G., Illera, J. C. and Illera, M. (1993). Bovine oocytes maturation and fertilization in-vitro with growth factors and oestrus cow serum. Theriog., 39: 262.
    10. Martins, R. D., Costa, E. P., Chagas, J. S. C., Ignacio, F. S., Torres, C. A. A. and McManus, C. (2005). Effect of vitrification of immature bovine oocytes on in-vitro maturation. Anim. Reprod. Sci.,2: 128–134.
    11. Ocana-Quero, J. M., Pinedo Merlin, M., Ortega-Mariscal, M. and Moreno Millan, Y. M. (1999). The effect of different sera and bovine serum albumin fraction (BSA) on in-vitro maturation of immature bovine oocytes. Archivos De Zootecnia 48: 167-174.
    12. Rose, T. A. and Bavister, B. D. (1992). Effect of oocyte maturation medium on in-vitro development of in-vitro fertilized bovine embryos. Mol. Reprod. Develop., 31: 72-77.
    13. Shea, B. F., Latour, J. P.A., Bedirian, K. N. and Baker, R. D. (1976). Maturation in-vitro and subsequent penetrability of bovine follicular oocytes. J. Anim. Sci., 43: 809-815.
    14. Skinner, M. K. (1990). Mesenchymal (stromal) – epithelial cell interaction in the testis and ovary which regulate gonadal function. Reprod. Fert. Develop.,2: 237-244.
    15. Totey, S. M., Singh, G., Taneja, M., Pawshe, C. H. and Talwar, G. P. (1992). In-vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). J. Reprod. Fert., 95: 597-607.
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