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Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 48 issue 6 (december 2014) : 521-526

Z-CHROMOSOME LINKED DINUCLEOTIDE STRS: ASSOCIATION WITH REPRODUCTION TRAITS IN CHICKEN

Jasdeep Singh, C.S. Mukhopadhyay*, G.S. Brah, Samita Saini1
1School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India
Cite article:- Singh Jasdeep, Mukhopadhyay* C.S., Brah G.S., Saini1 Samita (2025). Z-CHROMOSOME LINKED DINUCLEOTIDE STRS: ASSOCIATION WITH REPRODUCTION TRAITS IN CHICKEN. Indian Journal of Animal Research. 48(6): 521-526. doi: 10.5958/0976-0555.2014.00024.7.

ABSTRACT

The study was conducted on 96 unrelated chickens belonging to three strains, namely, White Leghorn (PL2), Punjab Broiler (PB2) and Punjab Red (PR). Five pairs of Z-chromosome linked microsatellite loci (Ase50, MCW154, HUR0404, HUR0411 and HUR0412) were studied in the selected strains with an aim to genetically characterize the divergent stocks and to associate the polymorphism with growth and production traits. The microsatellite band patterns were resolved using 3.5% metaphore agarose gel at 80-100 volts. The number of alleles in the microsatellite loci varied from 8 to 10 among the different germplasms, indicating high degree of polymorphism across the three breeds. Locus HUR0412 had the maximum number of alleles (10), thus it is the most polymorphic among all the loci under study. The allele ‘I’ of the locus HUR0412 is the candidate private allele that could be used to characterize PL2 breed. Except for the HUR0412 locus no other loci could be characterised for harbouring strain specific private allele. The PIC value was the highest in loci as: Ase50 in PL2 (0.715), MCW154 in PR (0.739) and PB2 (0.827). The HUR0412 locus exhibited significant difference among different genotypes of layer (PL2 and PR) females for the traits like, body weight at 40 weeks of age (BWT40), egg no. at 40 weeks (ENO40), age of sexual maturity (ASM) and rate of lay. The performance trait, egg mass (EMASS) was significantly different (P£0.05) among the genotypes of layers for the locus MCW154. The Chi-square test for various loci and breeds were significant i.e. the population were not in Hardy-Weinberg equilibrium.

REFERENCES

  1. Backstrom. N.; Brandstrom, M.; Gustafsson, L.; Qvarnstrom, A.; Cheng, H. and Ellegren, H. (2006). Genetic mapping in a natural population of collared flycatchers (Ficedula albicollis): conserved synteny but gene order rearrangements on the avian Z chromosome. Genetics 174: 377–86.
  2. Ben-Avraham, D.; Blum, S.; Granevitze, Z.W. S.; Cheng, H. and Hillel, J. (2006). W-specefic microsatellite loci detected by in silico analysis map to chromosome Z of the chicken genome. Anim. Genet. 37:179–88.
  3. Botstein, D.; White, R.L. Skolnick, M. and Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32: 314-31.
  4. Chatterjee, R.N.; Sharma, R.P.; Mishra, A.; Dange, M. and Bhattacharya, T.K.; (2008). Variability of microsatellites and their association with egg production traits in chicken. Int. J. Poultry Sci. 7: 77–80.
  5. Crooijmans, R.P.M.A.; Dijkhof, R.J.M.; Poel, J.J. and Groenen, M.A.M. (1997). New microsatellite markers in chicken optimised for automated fluorescent genotyping. Anim. Genet. 28: 427-37.
  6. Dawson, D.A.; Chittock, J.; Jehle, R.; Whitlock, A.; Nogueira, D. and Pellatt, J. (2005). Identification of 13 polymorphic microsatellite loci in the zebra finch, Taeniopygia guttata (Passeridae, Aves). Mol. Ecol. Notes 5: 298-301
  7. Harvey, W.R.; (1975). Least-squares analysis of data with unequal subclass numbers. ARS H-4, USDA.
  8. Hassen, H.; Neser, F.W.C.; Kock, A. and Marle-Koster, E. (2009). Study on the genetic diversity of native chickens in northwest Ethiopia using microsatellite markers. Afr. J. Biotechnol. 8(7): 1347-53.
  9. Ismoyowati and Purwantini D. (2010). An estimation of genetic variation in Indonesian local duck using microsatellite marker. Asian J. Poultry Sci. 4(4): 198-204.
  10. Kimura, M. and Crow, J.F. (1964). The number of alleles that can be maintained in a finite population. Genetics 49: 725-38.
  11. Lewontin, R.C. (1972). The apportionment of human diversity. In: Dobzhansky T, Hecht M K and Steere W C. (Ed) 1972. Evolutionary Biology 6 pp 81–98. New York: Appleton-Century-Crofts.
  12. McDonald, G. (2003). Biogeography: Space, Time and Life. John Wiley & Sons inc. First Edition: 409
  13. Roushdy, K.; El-Dein, A.Z.; Fathi, M.M.; Ali, U.M. and Assy, H.M. (2008). Microsatellite genetic differentiation analysis of two local chicken breeds compared with foreign Hy-Line strain. Int. J. Poultry Sci. 7(11): 1045-53.
  14. Sambrook, J. and Russell, D.W. (2001). Molecular cloning: A laboratory manual. 3rd Edn. 1: 6.4. Cold Spring Harbor Laboratory, Cold Spring, NY.
  15. Sasaki, O.; Odawara, S.; Takahashi, H.; Nirasawa, K.; Oyamada, Y.; Yamamoto, R.; Ishii, K.; Nagamine, Y.; Takeda, H.; Kobayashi, E.; and Furukawa, T. (2004). Genetic mapping of quantitative trait loci affecting body weight, egg character and egg production in F2 intercross chickens. Anim. Genet. 35: 188-194.
  16. Tautz, D. (1989).  Hypervariability of simple sequences as a general source for polymorphic DNA markers.  Nucleic Acids Res. 17: 6463-71.
  17. Tuiskula-Haavisto, Honkatukia, M.M.; Vilkki, J.; Koning, D.J.; Schulman, N.F.; and Ki-Tanil M. (2002). Mapping of quantitative trait loci affecting and production traits in egg layers. Poultry Sci. 81: 919-27.
  18. Wardecka, B.; Olszewski, R.; Jaszczak, K.; Zieba, G.; Pierzchala, M. and Wicinska K. (2002). Relationship between microsatellite marker alleles on chromosomes 1-5 originating from the Rhode Island Red and Green-legged Partrigenous breeds and egg production and quality traits in F2 mapping population. J. Appl. Genet. 43(3): 319-29.
  19. Zhou, Y.; Liu, Y.P.; Kang, L.; Meng, X.J.; Li, L. and Zhu; Q. (2008). Correlation between microsatellite loci and onset of lay and egg quality traits in Chinese Silkies, Gallus gallus. J. Poultry Sci. 45: 241-48.
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