DOI: 10.5958/0976-0555.2014.00039.9    | Article Id: B-2487 | Page : 601-604
A.R. Gupta*1, S. Dey1, M. Saini2 and D. Swarup3 dramitrajgupta@gmail.com
Address : Environmental Medicine Laboratory, Division of Medicine, Indian Veterinary Research Institute, Izatnagar - 243122, India


Excessive ingestion of fluoride leads to abnormal mineralization of both enamel and dentin. Mechanisms about enamel fluorosis have been clarified. The knowledge about dentin fluorosis is limited and the mechanism is unclear. The present experiment was aimed to ascertain the excessive ingestion of fluoride on collagen metabolism in dentin of incisor teeth of rats. Rats (n=12) were divided into two groups. Rats of group I served as control and received only tap water and those of group II received sodium fluoride @ 200 mg/L of drinking water ad lib for a period of 90 days. Quantitative real-time polymerase chain reaction was used to quantify the gene expression levels of Collal in dentin. Significant decreases in expression of Collal gene in the dentin of incisor teeth of fluoride exposed rats was noted at the end of the experiment.


Collal gene Dentin Fluoride Rats.


  1. Bely M., Pinter T., Sandorfi Nora. and Ratko I. (1988). Changes in the collagen structure of bone tissue in experimental fluorosis. Fluoride, 21: 28-31.
  2. Dey S., Swarup D., Saxena A. and Dan A. (2011). In vivo efficacy of tamarind (Tamarindus indica) fruits extract on experimental fluoride exposure in rats. Res Vet Sci, 91: 422-425.
  3. Gibson U.E., Heid C.A. and Williams P.M. (1996). A novel method for real time quantitative RT-PCR. Genome Res, 6: 995-1001.
  4. Han T.L., Wang M., Yan X.Y., Niu R.Y. and Wang J.D. (2010). Decreased expression of type I collagen (COL1A1) and dentin phosphoprotein (DPP) in teeth of fluorosed sheep. Fluoride, 43: 19-25.
  5. Kirsch T., Harrison G., Golub E.E. and Nah H.D. (2000). The roles of annexins, types II and X collagen in matrix vesicle- mediated mineralization of growth plate cartilage. J Biol Chem, 275: 35577-35583.
  6. Li W.T., Yang .LF., Ren Y.C., Yan X.Y. and Wang J.D. (2007). Quantification of rib COL1A2 gene expression in healthy and fluorosed Inner Mongolia cashmere goats. Fluoride, 40: 13-18.
  7. Linde A. and Goldberg M. (1993). Dentinogenesis. Crit Rev Oral Biol Med, 4: 679-728
  8. Linde A., Bhown M. and Butler W.T. (1980). Noncollagenous proteins of dentin. A re-examination of proteins from rat incisor dentin utilizing techniques to avoid artifacts. J Biol Chem, 255: 5931-5942.
  9. Miu Q., Xu M., Liu B. and You B. (2002). In vivo and in vitro study on the effect of excessive fluoride on type I collagen of rats. J Hyg Res, 31: 145-147. Pfaffl M.W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res, 29: 2002-2007.
  10. Pu C.J., Hou L.H. and Yang S.H. (1996). Effects of excessive fluoride on metabolism of collagen protein in cartilage matrix. Chin J Control Endem Dis, 11: 75–77.
  11. Ranjan R., Swarup D., Patra R.C. and Chandra V. (2009). Tamarindus indica L. and Moringa oleifera M. extract administration ameliorate fluoride toxicity in Rabbits. Indian J Exp Biol, 47: 900-905.
  12. Semevolos S.A., Nixon A.J. and Brower-Toland B.D. (2001). Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta l in articular cartilage obtained from horses with naturally acquired osteochondrosis. Am J Vet Res, 62: 1088-1094.
  13. Susheela A.K. and Jha M. (1981). Effects of Fluoride on Cortical and Cancellous Bone Composition. IRCS Med Sci Lib Comp, 9: 1021-1022.
  14. Susheela A.K. and Mukerjee D. (1981). Fluoride poisoning and the Effect of Collagen Biosynthesis of Osseous and Nonosseous Tissue. Toxicol Eur Res, 3: 99-104.
  15. Susheela A.K. and Sharma Y.D. (1982). Certain facets of F- action on collagen protein in osseous and nonosseous tissues. Fluoride, 15: 177-190.
  16. Wang M., Han T.L., Cao C.F. and Wang J.D. (2010). Effects of aluminum (Al) and fluoride (F) on the expression of type I collagen (COL1A1) in the teeth of guinea pigs. Fluoride, 43: 45-52.
  17. Wardas M., Jurczak T., Paw³owska-Góral K. and Kotrys-Puchalska E. (2002). Effects of fluoride and ascorbic acid on collagen biosynthesis in mouse liver fibroblast cultures. Fluoride, 35: 104-109.
  18. Xu Z.L., Liu B.C. and Miu, Q. (2003). Effects of excessive fluoride on the expression of type I collagen mRNA and fiber characteristics in rat bone. J Environ Health, 20: 204–207.
  19. Yan X.Y., Li W.T., Zhou B.H., Wang J.M. and Wang J.D. (2007). Effect of supplemented protein and Ca nutrition on fluoride-induced disturbance of rib COL1A1 gene expression in rabbits. Fluoride, 40: 140-148.

Global Footprints