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Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 3 (june 2015) : 355-359,   Doi: 10.5958/0976-0555.2015.00049.7

In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows

J
Jing Zhao*
J
Jun Wang
Y
Yujiang Yang
X
Xiangnan Li
C
Chengtao Sun
1College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Cite article:- Zhao* Jing, Wang Jun, Yang Yujiang, Li Xiangnan, Sun Chengtao (2025). In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows. Indian Journal of Animal Research. 49(3): 355-359. doi: 10.5958/0976-0555.2015.00049.7.

ABSTRACT

The present study was conducted to isolate lactic acid bacteria (LAB) from vaginas of cows and evaluate their properties as probiotics. Forty-seven LAB strains were isolated from the vagina of cows using MRS agar and then their antibacterial effects were evaluated using Oxford cup method. Four strains (LAB1, LAB3, LAB5 and LAB9)demonstrating antibacterial activity were identified as Weissella confusa, Enterococcus hirae, Leuconostoc lactis and Ilyobacter polytropus, respectively.The results showed that the rank of hydrophobicity and adherence ability from largest to smallest was LAB9, LAB5, LAB3 and LAB1, respectively. Results of different combinations of the four LAB strains showed that LAB1+LAB3expressed higher adherence ability than other combinations (p£0.05); and LAB1+LAB9 had higher antibacterial activity than other combinations(p£0.05). Results of the present study provide essential information for developing probiotic product to prevent and treat reproductive tract infections of cows.

REFERENCES

  1. Borchert, D., Sheridan, L., Papatsoris, A., Faruquz, Z., Barua, J., Junaid, I., Pati, Y., Chinegwundoh, F. and Buchholz, N. (2008). Prevention and treatment of urinary tract infection with probiotics: Review and research perspective.Indian J Urol,24: 139–144.
  2. Boris, S., Suárez, J.E., Vázquez, F. and Barbés, C. (1998). Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun,66:1985-1989.
  3. Charteris, W.P., Kelly, P.M., Morelli, L. and Collins, J.K. (2001). Antibacterial activity associated with Lactobacillus gasseri ATCC 9857from the human female genitourinary tract. World J Microb Biot,17: 615-625.
  4. Collado, M., Meriluoto, J. and Salminen, S. (2007). Development of new probiotics by strain combinations: is it possible to improve the adhesion to intestinal mucus? J Dairy Sci, 90: 2710-2716.
  5. Corr, S.C., Li, Y., Riedel, C.U., O’Toole, P.W., Hill, C. and Gahan, C.G. (2007). Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118. P Natl Acad Sci USA,104: 7617-7621.
  6. Geertsema-Doornbusch, G., Van der Mei, H. and Busscher, H. (1993). Microbial cell surface hydrophobicity the involvement of electrostatic interactions in microbial adhesion to hydrocarbons (MATH). J Microbiol Meth,18: 61-68.
  7. Kaewsrichan, J., Peeyananjarassri, K. and Kongprasertkit, J. (2006). Selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. Fems Immunol Med Mic,48: 75-83.
  8. Lamont, R.F., Sobel, J.D., Akins, R.A., Hassan, S.S., Chaiworapongsa, T., Kusanovic, J.P. and Romero, R. (2011). The vaginal microbiome: new information about genital tract flora using molecular based techniques. Bjog-Int J Obstet Gy,118: 533-549.
  9. LeBlanc, S.J. (2008). Postpartum uterine disease and dairy herd reproductive performance: a review. Vet J,176: 102-114.
  10. Nader-Macías, M.E.F., Otero, M.C., Espeche, M.C. and Maldonado, N.C. (2008). Advances in the design of probiotic products for the prevention of major diseases in dairy cattle. J Ind Microbiol Biot,35: 1387-1395.
  11. Otero, M., Morelli, L. and Nader Macías, M. (2006). Probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle. Lett Appl Microbiol,43: 91-97.
  12. Pascual, L., Ruiz, F., Giordano, W. and Barberis, I.L. (2010). Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection. Indian J Med Microbi,59: 360-364.
  13. Rodriguez, C., Cofre, J., Sanchez, M., Fernandez, P., Boggiano, G. and Castro, E. (2011). Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle. Anaerobe,17: 15-18.
  14. Rogosa, M. and SHARPE, M.E. (1960). Species differentiation of human vaginal lactobacilli. J Gen Microbiol, 23: 197-201.
  15. Rosenberg, M., Judes, H. and Weiss, E. (1983). Cell surface hydrophobicity of dental plaque microorganisms in situ. Infect Immun,42: 831-834.
  16. Sheldon IM, Cronin J, Goetze L, Donofrio G, Schuberth HJ. (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity. Biol Reprod,81: 1025-1032.
  17. Wang, Y., Ametaj, B.N., Ambrose, D.J. and Gänzle, M.G. (2013). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. BMC Microbiol,13:19.
  18. Wood, J.R., Sweet, R.L., Catena, A., Hadley, W.K. and Robbie, M. (1985). In vitro adherence of Lactobacillus species to vaginal epithelial cells. Am J Obstet Gynecol,153: 740-743.
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    volume 49 issue 3 (june 2015) : 355-359,   Doi: 10.5958/0976-0555.2015.00049.7

    In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows

    J
    Jing Zhao*
    J
    Jun Wang
    Y
    Yujiang Yang
    X
    Xiangnan Li
    C
    Chengtao Sun
    1College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
    Cite article:- Zhao* Jing, Wang Jun, Yang Yujiang, Li Xiangnan, Sun Chengtao (2025). In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows. Indian Journal of Animal Research. 49(3): 355-359. doi: 10.5958/0976-0555.2015.00049.7.

    ABSTRACT

    The present study was conducted to isolate lactic acid bacteria (LAB) from vaginas of cows and evaluate their properties as probiotics. Forty-seven LAB strains were isolated from the vagina of cows using MRS agar and then their antibacterial effects were evaluated using Oxford cup method. Four strains (LAB1, LAB3, LAB5 and LAB9)demonstrating antibacterial activity were identified as Weissella confusa, Enterococcus hirae, Leuconostoc lactis and Ilyobacter polytropus, respectively.The results showed that the rank of hydrophobicity and adherence ability from largest to smallest was LAB9, LAB5, LAB3 and LAB1, respectively. Results of different combinations of the four LAB strains showed that LAB1+LAB3expressed higher adherence ability than other combinations (p£0.05); and LAB1+LAB9 had higher antibacterial activity than other combinations(p£0.05). Results of the present study provide essential information for developing probiotic product to prevent and treat reproductive tract infections of cows.

    REFERENCES

    1. Borchert, D., Sheridan, L., Papatsoris, A., Faruquz, Z., Barua, J., Junaid, I., Pati, Y., Chinegwundoh, F. and Buchholz, N. (2008). Prevention and treatment of urinary tract infection with probiotics: Review and research perspective.Indian J Urol,24: 139–144.
    2. Boris, S., Suárez, J.E., Vázquez, F. and Barbés, C. (1998). Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun,66:1985-1989.
    3. Charteris, W.P., Kelly, P.M., Morelli, L. and Collins, J.K. (2001). Antibacterial activity associated with Lactobacillus gasseri ATCC 9857from the human female genitourinary tract. World J Microb Biot,17: 615-625.
    4. Collado, M., Meriluoto, J. and Salminen, S. (2007). Development of new probiotics by strain combinations: is it possible to improve the adhesion to intestinal mucus? J Dairy Sci, 90: 2710-2716.
    5. Corr, S.C., Li, Y., Riedel, C.U., O’Toole, P.W., Hill, C. and Gahan, C.G. (2007). Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118. P Natl Acad Sci USA,104: 7617-7621.
    6. Geertsema-Doornbusch, G., Van der Mei, H. and Busscher, H. (1993). Microbial cell surface hydrophobicity the involvement of electrostatic interactions in microbial adhesion to hydrocarbons (MATH). J Microbiol Meth,18: 61-68.
    7. Kaewsrichan, J., Peeyananjarassri, K. and Kongprasertkit, J. (2006). Selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. Fems Immunol Med Mic,48: 75-83.
    8. Lamont, R.F., Sobel, J.D., Akins, R.A., Hassan, S.S., Chaiworapongsa, T., Kusanovic, J.P. and Romero, R. (2011). The vaginal microbiome: new information about genital tract flora using molecular based techniques. Bjog-Int J Obstet Gy,118: 533-549.
    9. LeBlanc, S.J. (2008). Postpartum uterine disease and dairy herd reproductive performance: a review. Vet J,176: 102-114.
    10. Nader-Macías, M.E.F., Otero, M.C., Espeche, M.C. and Maldonado, N.C. (2008). Advances in the design of probiotic products for the prevention of major diseases in dairy cattle. J Ind Microbiol Biot,35: 1387-1395.
    11. Otero, M., Morelli, L. and Nader Macías, M. (2006). Probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle. Lett Appl Microbiol,43: 91-97.
    12. Pascual, L., Ruiz, F., Giordano, W. and Barberis, I.L. (2010). Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection. Indian J Med Microbi,59: 360-364.
    13. Rodriguez, C., Cofre, J., Sanchez, M., Fernandez, P., Boggiano, G. and Castro, E. (2011). Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle. Anaerobe,17: 15-18.
    14. Rogosa, M. and SHARPE, M.E. (1960). Species differentiation of human vaginal lactobacilli. J Gen Microbiol, 23: 197-201.
    15. Rosenberg, M., Judes, H. and Weiss, E. (1983). Cell surface hydrophobicity of dental plaque microorganisms in situ. Infect Immun,42: 831-834.
    16. Sheldon IM, Cronin J, Goetze L, Donofrio G, Schuberth HJ. (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity. Biol Reprod,81: 1025-1032.
    17. Wang, Y., Ametaj, B.N., Ambrose, D.J. and Gänzle, M.G. (2013). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. BMC Microbiol,13:19.
    18. Wood, J.R., Sweet, R.L., Catena, A., Hadley, W.K. and Robbie, M. (1985). In vitro adherence of Lactobacillus species to vaginal epithelial cells. Am J Obstet Gynecol,153: 740-743.
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