Protective effects of sodium selenite against aflatoxin B1 on haemoglobin content, erythrocyte count, and immune adherence function in broilers

DOI: 10.5958/0976-0555.2015.00047.3    | Article Id: B-243 | Page : 360-363
Citation :- Protective effects of sodium selenite against aflatoxin B1 on haemoglobin content, erythrocyte count, and immune adherence function in broilers.Indian Journal Of Animal Research.2015.(49):360-363
Liao Tingting*, Yuan Shibin*, Zhang Zejun and Yuan Guiping yshibin@sina.com
Address : Giant Panda Conservation and Culture Research Center, China West Normal University, Nanchong, Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education) Nanchong, 637002, China.

Abstract

Aflatoxin B1 (AFB1), commonly found in feed stuffs causes hepatocellular carcinoma and immunosuppression. Selenium (Se) is mainly known as an important antioxidant and immunostimulant. To evaluate the protective effects of dietary sodium selenite against AFB1_induced erythocytic toxicology, one hundred male Avian broilers were randomly divided into five groups viz. : fed with basal diet (control group), 0.3mg/kg AFB1 (AFB1 group), 0.3mg/kg AFB1+0.2mg/kg Se (+Se group ‘!), 0.3mg/kg AFB1+0.4mg/kg Se (+Se group a!) and 0.3mg/kg AFB1+0.6mg/kg Se (+Se group b!), respectively. At 7, 14 and 21 days of the experiment, the red blood cells (RBC count), content of hemoglobin (Hb) and the immune adherence function were determined. Compared with control group, the number of RBC in AFB1 group was increased, while the content of Hb and erythrocyte rosette rates in AFB1 group were decreased significantly. Dietary sodium selenite, however, could decrease the number of RBC; increase the content of Hb and erythrocyte rosette rate significantly. It was indicated that sodium selenite in the diets could exert protective effects against AFB1-induced damage on the red blood cells, and the transport capacity and immune adherence function of erythocyte could be maintained.

Keywords

Aflatoxin B1 Broiler Haemoglobin Red Blood Cell Rosette rate Sodium selenite.

References

  1. Brucato M, Sundlof SF, Bell JU, and Edds GT (1986). Aflatoxin B1 toxicosis in dairy calves pretreated with selenium- vitamin E. Am J Vet Res ,47(1): 179-183.
  2. Chen KJ, Shu G, Peng X, Fang J, Cui HM, Chen J, Wang FY, Chen ZL, Zuo ZC, Deng JL, Geng Y, and Lai WM (2013). Protective role of sodium selenite on histopathological lesions, decreased T-cell subsets and increased apoptosis of thymus in Broilers intoxicated with aflatoxin B1. Food and Chem Toxicol ,59: 446-454.
  3. Choi KC, Chung WT, Kwon JK, Yu JY, Jang YS, Park SM, Lee SY and Lee JC (2010). Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in mice. Food Chem Toxicol, 48(10): 2747-2753.
  4. Cohen JHM, Atkinson JP, Klickstein LB, Oudin S, Subramanian VB and Moulds JM (1999) .The C3b/C4b receptor (CR1, CD35) on erythrocytes: methods for study of the polymorphisms. Mol Immunol ,36(13): 819-825.
  5. Deng YB, Cui HM, Peng X, Fang J, Zuo ZC, Deng JL and Lou Q. (2013). Effects of high dietary fluorine on erythrocytes and erythrocyte immune adherence function in broiler chickens. Bio Trace Elem Res, 155(2): 247-252.
  6. Dönmez N, Dönmez HH, Keskin E and Kisadere Ý (2012). Effects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams. Sci World J , (2012): 1-4.
  7. Fernandez A, Ramos JJ and Sanz MCT (1996). Alterations of the performance, hematology and clinical biochemistry of growing lambs fed with aflatoxin in the diet. J Appl Toxicol ,16(1): 85-91.
  8. Guo F (1982) Initial research of RBC immune function [J]. National Medical Journal of Chinese, 62(3): 715-716.
  9. Gürgöze MK, Aygün AD, Ölçücü A, Doðan Y and Yýlmaz E (2004) Plasma selenium status in children with iron deficiency anemia. J Trace Elem Med Biol ,18(2): 193-196.
  10. Gürgöze MK, Ölçücü A, Aygün AD, Taºkin E and Kiliç M (2006). Serum and hair levels of zinc, selenium, iron, and copper in children with iron-deficiency anemia. Biol Trace Elem Res ,111(1-3): 23-29.
  11. Hussain I, Anwar J, Asi MR, Munawar MA and Kashif M (2010) Aflatoxin M1 contamination in milk from five dairy species in Pakistan. Food Control ,21(2): 122-124.
  12. Keshacarz SA, Memarbashi A and Balali M (2001). Preventive effect of selenium on T-2 toxin membrane toxicity. Adv Exp Med Biol ,500: 463-466.
  13. Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R and Gladyshev VN (2003). Characterization of mammalian selenoproteomes. Sci 300(5624): 1439-1443.
  14. Ma HD (2004). Experimental course of physiology. Sichuan Science and Technology Press, Chengdu, China.
  15. Paccaud JP, Carpentier JL and Schifferli JA (1990). Difference in the clustering of complement receptor type 1 (CR1) on polymorphonuclear leukocytes and erythrocytes: effect on immune adherence. Eur J Immunol ,20(2): 283-289.
  16. Pasricha SS, Flecknoe-Brown SC and Allen KJ (2010). Diagnosis and management of iron deficiency anaemia: a clinical update. Med J Aust, 193: 525-532.
  17. Raju MVLN and Devegowda G (2000). Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). Br Poult Sci ,41(5): 640-650.
  18. Shen HM, Ong CN and Shi CY (1995). Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes. Toxicol, 99(1): 115-123.
  19. Steinbrenner H, Sies H (2009). Protection against reactive oxygen species by selenoproteins. Biochim Biophys Acta ,1790(11): 1478-1485.
  20. Surai PF (2002). Selenium in poultry nutrition: A new look at an old element. 1. Antioxidant properties, deficiency and toxicity. Worlds Poult Sci ,58: 333-347.
  21. Toghyani M, Tohidi M, Gheisari AA and Tabeidian SA (2010). Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. Afr J Biotechnol, 9(40):6819-6825
  22. Verma RJ and Raval PJ (1992). Alterations in erythrocytes during induced chronic aflatoxicosis in rabbits. Bulletin Environ Contam Toxicol ,49(6): 861-865.
  23. Wang FY, Shu G, Peng X, Fang J, Chen KJ, Cui HM, Chen ZL, Zuo ZC, Deng JL, Geng Y and Lai WM (2013). Protective effects of sodium selenite against aflatoxin B1-induced oxidative stress and apoptosis in broiler spleen. Int J Environ Res Public Health, 10(7): 2834-2844.

Global Footprints