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volume 48 issue 5 (october 2014) : 403-407,   Doi: 10.5958/0976-0555.2014.00001.6

IDENTIFICATION OF GENETIC POLYMORPHISM IN TWO EXONIC CODING REGION OF LEPTIN GENE AMONG INDIGENOUS AND CROSSBRED CATTLE

U
Umesh Singh*
S
Sushil Kumar
I
Indrajit Ganguly
G
G.K. Gaur
J
Jagadeesan K.
S
Sunil Kumar
S
Sandeep Mann
R
Rani Singh
1Molecular Genetics Laboratory, Central Institute for Research on cattle, Meerut Cantt- 250 001, India
Cite article:- Singh* Umesh, Kumar Sushil, Ganguly Indrajit, Gaur G.K., K. Jagadeesan, Kumar Sunil, Mann Sandeep, Singh Rani (2025). IDENTIFICATION OF GENETIC POLYMORPHISM IN TWO EXONIC CODING REGION OF LEPTIN GENE AMONG INDIGENOUS AND CROSSBRED CATTLE. Indian Journal of Animal Research. 48(5): 403-407. doi: 10.5958/0976-0555.2014.00001.6.

ABSTRACT

Leptin gene has its role in appetite, metabolism, growth and milk production in cattle. PCR-RFLP method was carried out to detect the polymorphism in leptin gene among Frieswal, Ongole and Sahiwal cattle. Frieswal population under study revealed that 11%, 51% and 38% are TT, CT, and CC respectively genotypes with respect to exon 2. But the same population had 22% TT, 51%  CT and 27% CC genotype for first SNP in exon 3; 4% TT, 38% C Tand 58% CC genotype for second SNP in exon 3. Ongole population revealed only CT genotype with respect to exon 2, but it showed TT genotype for first SNP and CC genotype for second SNP in exon3. Sahiwal population had 4% TT, 88% CT and 8% CC genotype (exon 2), respectively. Whereas the same population revealed 4% TT, 55% CT and 41% CC genotype for first SNP in exon 3 and 4% CT and 96% CC genotype for second SNP in exon 3. Sahiwal population had no TT genotype for second SNP in exon 3.

KEYWORDS

    REFERENCES

    1. Aslaminejad Asghar Ali, Mohammad Reza Nassiry, Hadi Farajollahi, Morteza Mahdavi, Hasan Abbasi, Ali Javadmanesh (2010). Polymorphism in Exon 3 of Leptin Gene in Iranian native cattle breeds.J. Appl. Anim. Res. 37: 159-162
    2. Blache D, Tellam R L, Chagas L M, Blackberry M A, Vercoe P E, Martin G B (2000). Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep, J. Endocrinol.165: 625-637.
    3. Buchanan, F.C, C.J Fitzsimmons, A.G. van Kessel, T.D. Thue, D.C.W Sim and S.M. Schmutz (2002). Association of a missense mutation in the bovine leptin gene with earcass fat content and leptin mRNA levels. Genet. Sel. Evol., 34: 105-116.
    4. Chilliard, Y, Delavaud, C and Bonnet, M (2005). Leptin expression in ruminants: Nutritional and physiological regulations in relation with energy metabolism. Domestic. Anim. Endocrinol., 29: 3-22.
    5. Choudhary, V, P Kumar, T K Bhattacharya, B Bhushan and A Sharma (2005). DNA polymorphism of leptin gene in Bosindicus and Bostaurus cattle. J. Genet. Mol. Biol., 28: 740-742.
    6. Dandapat Anjan, Kumar D, Ghosh AK, Banerjee Dipak (2009). Association of leptin polymorphism with growth, milk production and reproduction traits in Sahiwal and crossbred cattle.India J. of Anim. Sci..79(9)
    7. Falconer, D. S., and T. F. C. Mackay(1996).Introduction to Quantitative Genetics, Ed 4.Longmans Green, Harlow, Essex, UK..
    8. He Y, Chen H, Quon M J and Reitman M (1995). The mouse obese gene: Genome organization, promoter activity, and activation by CCAAT/enhancer-binding protein alpha, J. Biol. Chem., 270:28887-28891
    9. Konfortov B A ,Licence V E , Miller J R (1999). Re-sequencing DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon, Mamm. Genome 10 1142-1145.
    10. Kulig H, Kmie M (2009). Association between Leptin Gene Polymorphisms and Growth Traits in Limousin Cattle.Russ. J. Genet., 45(6): 738-741.
    11. Lagonigro, Wiener R, P, F Pilla, Woolliams J A and Williams J L (2003).A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Genet., 34: 371-374.
    12. Lien, S, H Sundvold, H Klungland and D I Vage (1997).Two novel polymorphisms in the bovine obesity gene (OBS).J. Anim. Genet., 28: 245-245.
    13. Liefers SC, Te Pas MFW, Veerkamp RF, Van Der Lende T (2002). Associations between Leptin Gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in holstein heifers.J. Dairy. Sci. 85:1633.
    14. Madeja, Z., T. Adamowicz, A. Chmurzynska, T. Jankowski, J. Melonek, M. Switonski and T. Strabel, (2004).Effect of leptin gene polymorphisms on breeding value for milk production traits.J. Dairy Sci., 87: 3925-3927.
    15. Nassiry MR, Shahroudi FE, Mousavi AH, Sadeghi B, Javadmanesh A (2008).The diversity of leptin gene in Iranian native, Holstein and Brown Swiss cattle.Afr. J. Biotechnol., 7 (15): 2685-2687.
    16. Nkrumah, J D, Li, C, Yu, J, Hansen, C, Keislar, D H and Moore, S S (2005). Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior and measures of carcass merit. J. Animal. Sci., 83: 20-28
    17. Pomp D, Zou T, Clutter A C, Barendse W (1997). Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism.J. Anim. Sci. 75:1427.
    18. Stone R T, Kappes S M , Beattie C W (1996). The bovine homologue of the obese gene maps to chromosome 4, Mammal. Genome 7 : 399-400.
    19. Tellam, R L (2004). Direct Submission accession number BTU50365. CSIRO Division of tropical Animal Production, PMB 3, Indooroopilly, QLD 4068, Australia. http://www.ncbi.nlm.nih.gov
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    Indian Journal of Animal Research
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      volume 48 issue 5 (october 2014) : 403-407,   Doi: 10.5958/0976-0555.2014.00001.6

      IDENTIFICATION OF GENETIC POLYMORPHISM IN TWO EXONIC CODING REGION OF LEPTIN GENE AMONG INDIGENOUS AND CROSSBRED CATTLE

      U
      Umesh Singh*
      S
      Sushil Kumar
      I
      Indrajit Ganguly
      G
      G.K. Gaur
      J
      Jagadeesan K.
      S
      Sunil Kumar
      S
      Sandeep Mann
      R
      Rani Singh
      1Molecular Genetics Laboratory, Central Institute for Research on cattle, Meerut Cantt- 250 001, India
      Cite article:- Singh* Umesh, Kumar Sushil, Ganguly Indrajit, Gaur G.K., K. Jagadeesan, Kumar Sunil, Mann Sandeep, Singh Rani (2025). IDENTIFICATION OF GENETIC POLYMORPHISM IN TWO EXONIC CODING REGION OF LEPTIN GENE AMONG INDIGENOUS AND CROSSBRED CATTLE. Indian Journal of Animal Research. 48(5): 403-407. doi: 10.5958/0976-0555.2014.00001.6.

      ABSTRACT

      Leptin gene has its role in appetite, metabolism, growth and milk production in cattle. PCR-RFLP method was carried out to detect the polymorphism in leptin gene among Frieswal, Ongole and Sahiwal cattle. Frieswal population under study revealed that 11%, 51% and 38% are TT, CT, and CC respectively genotypes with respect to exon 2. But the same population had 22% TT, 51%  CT and 27% CC genotype for first SNP in exon 3; 4% TT, 38% C Tand 58% CC genotype for second SNP in exon 3. Ongole population revealed only CT genotype with respect to exon 2, but it showed TT genotype for first SNP and CC genotype for second SNP in exon3. Sahiwal population had 4% TT, 88% CT and 8% CC genotype (exon 2), respectively. Whereas the same population revealed 4% TT, 55% CT and 41% CC genotype for first SNP in exon 3 and 4% CT and 96% CC genotype for second SNP in exon 3. Sahiwal population had no TT genotype for second SNP in exon 3.

      KEYWORDS

        REFERENCES

        1. Aslaminejad Asghar Ali, Mohammad Reza Nassiry, Hadi Farajollahi, Morteza Mahdavi, Hasan Abbasi, Ali Javadmanesh (2010). Polymorphism in Exon 3 of Leptin Gene in Iranian native cattle breeds.J. Appl. Anim. Res. 37: 159-162
        2. Blache D, Tellam R L, Chagas L M, Blackberry M A, Vercoe P E, Martin G B (2000). Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep, J. Endocrinol.165: 625-637.
        3. Buchanan, F.C, C.J Fitzsimmons, A.G. van Kessel, T.D. Thue, D.C.W Sim and S.M. Schmutz (2002). Association of a missense mutation in the bovine leptin gene with earcass fat content and leptin mRNA levels. Genet. Sel. Evol., 34: 105-116.
        4. Chilliard, Y, Delavaud, C and Bonnet, M (2005). Leptin expression in ruminants: Nutritional and physiological regulations in relation with energy metabolism. Domestic. Anim. Endocrinol., 29: 3-22.
        5. Choudhary, V, P Kumar, T K Bhattacharya, B Bhushan and A Sharma (2005). DNA polymorphism of leptin gene in Bosindicus and Bostaurus cattle. J. Genet. Mol. Biol., 28: 740-742.
        6. Dandapat Anjan, Kumar D, Ghosh AK, Banerjee Dipak (2009). Association of leptin polymorphism with growth, milk production and reproduction traits in Sahiwal and crossbred cattle.India J. of Anim. Sci..79(9)
        7. Falconer, D. S., and T. F. C. Mackay(1996).Introduction to Quantitative Genetics, Ed 4.Longmans Green, Harlow, Essex, UK..
        8. He Y, Chen H, Quon M J and Reitman M (1995). The mouse obese gene: Genome organization, promoter activity, and activation by CCAAT/enhancer-binding protein alpha, J. Biol. Chem., 270:28887-28891
        9. Konfortov B A ,Licence V E , Miller J R (1999). Re-sequencing DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon, Mamm. Genome 10 1142-1145.
        10. Kulig H, Kmie M (2009). Association between Leptin Gene Polymorphisms and Growth Traits in Limousin Cattle.Russ. J. Genet., 45(6): 738-741.
        11. Lagonigro, Wiener R, P, F Pilla, Woolliams J A and Williams J L (2003).A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Genet., 34: 371-374.
        12. Lien, S, H Sundvold, H Klungland and D I Vage (1997).Two novel polymorphisms in the bovine obesity gene (OBS).J. Anim. Genet., 28: 245-245.
        13. Liefers SC, Te Pas MFW, Veerkamp RF, Van Der Lende T (2002). Associations between Leptin Gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in holstein heifers.J. Dairy. Sci. 85:1633.
        14. Madeja, Z., T. Adamowicz, A. Chmurzynska, T. Jankowski, J. Melonek, M. Switonski and T. Strabel, (2004).Effect of leptin gene polymorphisms on breeding value for milk production traits.J. Dairy Sci., 87: 3925-3927.
        15. Nassiry MR, Shahroudi FE, Mousavi AH, Sadeghi B, Javadmanesh A (2008).The diversity of leptin gene in Iranian native, Holstein and Brown Swiss cattle.Afr. J. Biotechnol., 7 (15): 2685-2687.
        16. Nkrumah, J D, Li, C, Yu, J, Hansen, C, Keislar, D H and Moore, S S (2005). Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior and measures of carcass merit. J. Animal. Sci., 83: 20-28
        17. Pomp D, Zou T, Clutter A C, Barendse W (1997). Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism.J. Anim. Sci. 75:1427.
        18. Stone R T, Kappes S M , Beattie C W (1996). The bovine homologue of the obese gene maps to chromosome 4, Mammal. Genome 7 : 399-400.
        19. Tellam, R L (2004). Direct Submission accession number BTU50365. CSIRO Division of tropical Animal Production, PMB 3, Indooroopilly, QLD 4068, Australia. http://www.ncbi.nlm.nih.gov
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