Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 49 issue 1 (february 2015) : 44-47

The PCR-SSCP and DNA sequencing methods detecting genetic mutations of EGLN1 gene in different sheep breeds

Rong-qing Geng*, Lan-ping Wang
1College of Life Science and Technology, Yancheng Teachers University, Yancheng, Jiangsu Province, 224051, P.R.China.
Cite article:- Geng* Rong-qing, Wang Lan-ping (2024). The PCR-SSCP and DNA sequencing methods detecting genetic mutations of EGLN1 gene in different sheep breeds. Indian Journal of Animal Research. 49(1): 44-47. doi: 10.5958/0976-0555.2015.00009.6.

ABSTRACT

In this study, genetic mutations of EGLN1 gene within four exons and intro-exon boundary regions were analyzed using PCR-SSCP and DNA sequencing methods representing different sheep breeds of high-altitude and low-altitude types. Only two SNPs located in intron 4 were detected among the screened regions and no species-specific SNP was found. The EGLN1 SNPs were found to be significantly different between the high-altitude and low-altitude sheep breeds revealing that genetic variations of EGLN1 gene may be related to high altitude adaption.

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