The PCR-SSCP and DNA sequencing methods detecting genetic mutations of EGLN1 gene in different sheep breeds

DOI: 10.5958/0976-0555.2015.00009.6    | Article Id: B-234 | Page : 44-47
Citation :- The PCR-SSCP and DNA sequencing methods detecting genetic mutations of EGLN1 gene in different sheep breeds.Indian Journal of Animal Research.2015.(49):44-47
Rong-qing Geng* and Lan-ping Wang rqgeng@hotmail.com
Address : College of Life Science and Technology, Yancheng Teachers University, Yancheng, Jiangsu Province, 224051, P.R.China.


In this study, genetic mutations of EGLN1 gene within four exons and intro-exon boundary regions were analyzed using PCR-SSCP and DNA sequencing methods representing different sheep breeds of high-altitude and low-altitude types. Only two SNPs located in intron 4 were detected among the screened regions and no species-specific SNP was found. The EGLN1 SNPs were found to be significantly different between the high-altitude and low-altitude sheep breeds revealing that genetic variations of EGLN1 gene may be related to high altitude adaption.


EGLN1 Genetic mutation PCR-SSCP Sheep.


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