Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 49 issue 2 (april 2015) : 199-204

Vaccaria segetalis activates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro

Nan L.I, Cheng-jie Wei, Hai-yang Meng, Xiao-xu Sun, Li-na Wang, Ye Lin, Xiao-han Yuan, Hui-li Tong, Qing-zhang L.I, Xue-jun Gao*
1Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University,Mucai Street 59, Xiangfang District, Harbin, 150030, Heilongjiang Province, China.
Cite article:- L.I Nan, Wei Cheng-jie, Meng Hai-yang, Sun Xiao-xu, Wang Li-na, Lin Ye, Yuan Xiao-han, Tong Hui-li, L.I Qing-zhang, Gao* Xue-jun (2024). Vaccaria segetalis activates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro. Indian Journal of Animal Research. 49(2): 199-204. doi: 10.5958/0976-0555.2015.00045.X.

ABSTRACT

Vaccaria segetalis has very strong effect on lactogenesis, but the mechanism is still unknown. This study aims to reveal the influence of Vaccaria segetalis on cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells (DCMECs). In this study the purified DCMECs was used to screen the most effective concentration of Vaccaria segetalis water decoction for lactogenesis. DCMECs were treated with prolactin (PRL), E (estrogen) or water extraction of Vaccaria segetalis, and many lactogenesis signal molecules in DCMECs including prolactin receptor (PRLR), estrogen receptor a (ERa), p-STAT5, p-mTOR, p100, GARS, PPARg and cyclinD1 were detected by using western blot assay. It was found that Vaccaria segetalis, E and PRL could promote lactogenesis of DCMECs and up-regulate these lactogenesis signal molecules except PPARg. These data reveals that Vaccaria segetalis has a similar role as E or PRL and activates cellular lactogenesis signaling pathway of DCMECs on both the translational and transcriptional levels.

REFERENCES

  1. Antonellis A, Ellsworth RE, Sambuughin N, Puls I, Abel A, Lee-Lin SQ, Jordanova A, Kremensky I, Christodoulou K, Middleton LT, Sivakumar K, Ionasescu V, Funalot B, Vance JM, Goldfarb LG, Fischbeck KH, and Green ED. (2003) Glycyl-tRNA synthetase mutations in Charcot-Tooth diease type 2D and distal spinal muscular atrophy type V. Am J Hum Grnet. 72(5):1293-1299.
  2. Bionaz M, Loor J. J. (2008) Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics. 9:366-387.
  3. Bionaz M, and Loor JJ. (2011) Gene networks driving bovine mammary protein synthesis during the lactation cycle. Bioinform Biol Insights. 5:83-98.
  4. Dash AB, Orrico FC, and Ness SA. (1996) The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb. Genes Dev. 10:1858–1869.
  5. Dimitrakakis C, Zhou J, Wang J, Matyakhina L, Mezey E, Wood JX, Wang D, and Bondy C. (2006) Co-expression of estrogen receptor-alpha and targets of estrogen receptor action in proliferating monkey mammary epithelial cells. Breast Cancer Research. 8:R10.
  6. Feng Y, Manka D, Wagner KU, and Khan SA. (2007) Estrogen receptor á expression in the mammary epithelium is required for ductal and alveolar morphogenesis in mice. Proceedings of the National Academy of Sciences of the USA. 104:14718-14723.
  7. Frech MS, Torre KM, Robinson GW, and Furth PA. (2008) Loss of cyclin D1 in concert with deregulated estrogen receptor alpha expression induces DNA damage response activation and interrupts mammary gland morphogenesis. Oncogene. 27:3186-3193.
  8. Huang JG, Gao XJÿLi QZ, Lu LM, Liu R, Luo CC, Wang JL, Qiao B and Jin X. (2012) Proteomic analysis of the nuclear phosphorylated proteins in diary cow mammary epithelial cells treated with estrogen. In Vitro Cellular & Developmental Biology-Animal. 48(7): 449-457
  9. Huang YL, Zhao F, Luo CC, Zhang X, Si Y, Sun Z, Zhang L, Li QZ, and Gao XJ. (2013) SOCS3-mediated blockade reveals major contribution of JAK2/STAT5 signaling pathway to lactation and proliferation of dairy cow mammary epithelial cells in vitro. Molecules. 18(10):12987-3002.
  10. Liang MY, Hou XM, Qu B, Zhang N, Li N, Cui YJ, Li QZ, and Gao XJ. (2014) Functional analysis of FABP3 in the milk fat synthesis signaling pathway of dairy cow mammary epithelial cells. In Vitro Cell Dev Biol Anim. 50(9):865-73.
  11. Lu L, Gao X, Li Q, Huang J, Liu R, and Li H. (2012) Comparative phosphoproteomics analysis of the effect of L-methionine on dairy cow mammary eptheliai cells. Canadian Journal of Animal Science. 92(4):433-442.
  12. Nilsson J, Bjursell G, and Kannius-Janson M. (2006) Nuclear JAK2 and transcription factor NF1-C2: a novel mechanism of prolactin signaling in mammary epithelial cells. Molecular and Cellular Biology. 26:5663-5674.
  13. Qi P, Zhang F, Xue R, Li Z, Chen M, Sun Z, Zhu K, and Huang C. (2014) Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent-separation strategy based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 28(11):1243-57
  14. Sobolewska A, Gajewska M, and Zarzyñska J. (2009) IGF-I, EGF, and sex steroids regulate autophagy in bovine mammary epithelial cells via the mTOR pathway. Eur. J. Cell Biol. 88: 117–130.
  15. Stingl J. (2011) Estrogen and progesterone in normal mammary gland development and in cancer. Horm Cancer. 2(2):85-90.
  16. Swaminathan G, Varghese B, and Fuchs SY. (2008) Regulation of prolactin receptor levels and activity in breast cancer. Journal of Mammary Gland Biology and Neoplasia. 13:81-91.
  17. Tong H, Gao X, Li Q, Liu J, Li N, and Wan Z. (2011) Metabolic regulation of mammary gland epithelial cells of dairy cow by galactopoietic compound isolated from Vaccaria segetails. Agricultural science in China. 10(7): 1106-1116.
  18. Valineva T, Yang J, Palovuori R, and Silvennoinen O. (2005) The transcriptional coacivator protein p100 recruits histone acetyl transferase activity to STAT6 and mediates interaction between the CREB-binding protein and STAT6. J. Biol.Chem. 280:14989–14996.
  19. Viitala S, Szyda J, Blott S, Schulman N, Lidauer M, Mäki-Tanila A, Georges M, and Vilkki J. (2006) The role of the bovine growth hormone receptor and prolactin receptor genes in milk, fat and protein production in Finnish Ayrshire dairy cattle. Genetics. 173:2151-2164.
  20. Wang X, Dong H, Liu Y, Yang B, Wang X, and Huang L. (2011) Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis. J Chromatogr B Analyt Technol Biomed Life Sci. 879(11-12):811-4.
  21. Wan Z, Tong H, Li Q, and Gao X. (2011) Influence on cellular signal transduction pathway in dairy cow mammary gland epithelial cells by galactopoietic compound isolated from Vaccaria Segetails. Agricultural science in China. 10(4):619-630.
  22. Yang J, Aittomäki S, Pesu M, Carter K, Saarinen J, Kalkkinen N, Kieff E, and Silvennoinen O. (2002) Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J. 21:4950-4958.
  23. Yang YH, Chen K, Li B, Chen JW, Zheng XF,Wang YR, Jiang SD, and Jiang LS. (2013) Estradiol inhibits osteoblast apoptosis via promotion of autophagy through the ER-ERK-mTOR pathway. Apoptosis. 18(11):1363-75.
  24. Yu C, Luo C, Qu Q, Khudhair N, Gu X, Zang Y, Wang C, Zhang N, Li Q, and Gao X. (2014) Molecular network including eIF1AX, RPS7, and 14-3-3ã regulates protein translation and cell proliferation in bovine mammary epithelial cells. Archives of Biochemistry and Biophysics. pii: S0003-9861(14)00348-8. [Epub ahead of print]
  25. Zhang X, Zhao F, Si Y, Huang YL, Yu CP, Luo CC, Zhang Na, Li QZ and Gao XJ. (2014) Negative feedback-loop regulation of GSK3â phosphorylation and mTOR/S6K1 signaling pathway independent of AKT to regulate milk synthesis and cell proliferation of dairy cow mammary epithelial cells. Molecules. 19(7): 9435-9452.
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