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Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.40

  • SJR 0.233, CiteScore: 0.606

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Indian Journal of Animal Research, volume 48 issue 4 (august 2014) : 315-321

CONSERVATION OF QINGHAI FINE-WOOL SHEEP AT SOMATIC CELL LEVEL IN STERILE CULTURE

Zhiqiang Shan1, 2*, Yabin Pu1*, Yuhua Gao1, Wenxiu Zhang1, Changli Li1, Mei Li1, Weijun Guan1#, Yuehui Ma1#
1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Cite article:- Shan1 Zhiqiang, 2*, Pu1* Yabin, Gao1 Yuhua, Zhang1 Wenxiu, Li1 Changli, Li1 Mei, Guan1# Weijun, Ma1# Yuehui (2025). CONSERVATION OF QINGHAI FINE-WOOL SHEEP AT SOMATIC CELL LEVEL IN STERILE CULTURE. Indian Journal of Animal Research. 48(4): 315-321. doi: 10.5958/0976–0555.2014.00449.X.

ABSTRACT

This study aimed to establish the fibroblast cell bank of Qinghai fine-wool sheep in the form of somatic cells, and to study its biological characteristics, to help for long-term conservation and sustainable use of this genetic resource. Ear marginal tissue of the Qinghai Fine-wool sheep was used to establish a fibroblast cell line by direct culture of explants and cryopreservation techniques, and the quality of the cell line was assessed. This study not only shows the effective preservation of the Qinghai Fine-wool sheep germplasm at the cellular level, but also provides a available experimental resource for fields including cell biology, genomics, post-genomics, genetic engineering and embryo engineering.

REFERENCES

  1. ATCC. (2010). Cell line misidentification: the beginning of the end. Nat Revi Cancer 10: 441-448.
  2. Bai, C., Wang, D., Li, C., Jin, D., Guan, W., and Ma, Y. (2011). Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank. Eur J of Histochem 55: e4.
  3. Corley-Smith, G.E., and Brandhorst, B.P. (1999). Preservation of endangered species and populations: a role for genome banking, somatic cell cloning, and androgenesis? Mol Reprod Dev 53:363-367.
  4. De Falco, E., Scafetta, G., Napoletano, C., Puca, R., Vingolo, E.M., Ragona, G., Iorio, O., and Frati, G. (2013). A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts. Cell Tissue Banking 14: 277-287.
  5. Eslaminejad, M.B., Vahabi, S., Shariati, M., and Nazarian, H. (2010). In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth. J Dent (Tehran) 7: 185-195.
  6. James, A.W., Levi, B., Nelson, E.R., Peng, M., Commons, G.W., Lee, M., Wu, B., and Longaker, M.T. (2011). Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo. Stem Cells and Development 20: 427-439.
  7. Kafri, R., Levy, J., Ginzberg, M.B., Oh, S., Lahav, G., and Kirschner, M.W. (2013). Dynamics extracted from fixed cells reveal feedback linking cell growth to cell cycle. Nature 494: 480-483.
  8. Kawarai, S., Hashizaki, K., Kitao, S., Nagano, S., Madarame, H., Neo, S., Ishikawa, T., Furuichi, M., Hisasue, M., Tsuchiya, R., et al. (2006). Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein. Veterinary Immunology and Immunopathology 113: 30-36.
  9. Kohler-Rollefson, I. (1997). Indigenous practices of animal genetic resource management and their relevance for the conservation of domestic animal diversity in developing countries. Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie 114: 231-238.
  10. Kozak, R.W. (1992). Introduction to the issues: recently developed methods for characterizing cell lines. Developments in Biological Standardization 76: 21-24.
  11. Lavania, U.C., and Sharma, A.K. (1981). An Interphase model for mitotic chromosome organization in eukaryota. Bio Systems 14: 171-178.
  12. Li, L.F., Guan, W.J., Hua, Y., Bai, X.J., and Ma, Y.H. (2009). Establishment and characterization of a fibroblast cell line from the Mongolian horse. In vitro cellular & developmental biology Animal 45: 311-316.
  13. Liu, Z., Wang, W., Gao, J., Zhou, H., and Zhang, Y. (2014). Isolation, culture, and induced multiple differentiation of Mongolian sheep bone marrow-derived mesenchymal stem cells. In Vitro Cell Dev Biol Animal.
  14. Muller, S., O’Brien, P.C., Ferguson-Smith, M.A., and Wienberg, J. (1997). A novel source of highly specific chromosome painting probes for human karyotype analysis derived from primate homologues. Hum Geneti 101: 149-153.
  15. Outani, H., Okada, M., Yamashita, A., Nakagawa, K., Yoshikawa, H., and Tsumaki, N. (2013). Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors. PloS one 8: e77365.
  16. Pylayeva-Gupta, Y., Lee, K.E., and Bar-Sagi, D. (2013). Microdissection and culture of murine pancreatic ductal epithelial cells. Methods Mol Biol 980: 267-279.
  17. Speirs, V., Ray, K.P., and Freshney, R.I. (1991). Paracrine control of differentiation in the alveolar carcinoma, A549, by human foetal lung fibroblasts. British Journal of Cancer 64: 693-699.
  18. Strober, W. (2001). Trypan blue exclusion test of cell Viability. Current protocols in immunology / edited by John E Coligan [et al] Appendix 3, Appendix 3B.
  19. Tong, H.L., Li, Q.Z., Gao, X.J., and Yin, D.Y. (2012). Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line. In Vitro Cell Devel Biolo Animal 48: 149-155.
  20. Uphoff, C.C., and Drexler, H.G. (2013). Detection of mycoplasma contaminations. Methods Mol Biol 946: 1-13.
  21. Washizu, T., Nakamura, M., Izawa, N., Suzuki, E., Tsuruno, S., Washizu, M., Nakajo, S., and Arai, T. (2002). The activity ratio of the cytosolic MDH/LDH and the isoenzyme pattern of LDH in the peripheral leukocytes of dogs, cats and rabbits. Vet Res Communi 26: 341-346.
  22. Werners, A.H., Bull, S., Fink-Gremmels, J., and Bryant, C.E. (2004). Generation and characterisation of an equine macrophage cell line (e-CAS cells) derived from equine bone marrow cells. Veterinary Immunology and Immunopathology 97: 65-76.
  23. Williamson, J., Marmion, B.P., Worswick, D.A., Kok, T.W., Tannock, G., Herd, R., and Harris, R.J. (1992). Laboratory diagnosis of Mycoplasma pneumoniae infection. 4. Antigen capture and PCR-gene amplification for detection of the Mycoplasma: problems of clinical correlation. Epidemiol Infecti 109: 519-537.
  24. Xiong, F., Mi, Z., and Gu, N. (2011). Cationic liposomes as gene delivery system: transfection efficiency and new application. Pharmazie 66: 158-164.
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