Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Investigation of the Prevalence of Brucella Diagnosis in Serum and Milk in Cattle

U. Ülker
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1Veterinary Control Center Research Institute Directorate, Ankara, Turkey.
Cite article:- Ülker U. (2022). Investigation of the Prevalence of Brucella Diagnosis in Serum and Milk in Cattle. Indian Journal of Animal Research. 56(9): 1154-1157. doi: 10.18805/IJAR.B-1404.
Background: Brucellosis is an infectious disease that affects both cattle and humans. Because brucellosis causes economic losses, serovalance studies are important in terms of eradication. Its diagnosis is integral to the design and implementation of preventive and management strategies for both cattle and humans. Various serological tests are commonly used in diagnostic processes.
Methods:This study was carried out in cattle farms located in Ankara, Çankırı, Kırşehir, Kayseri, Çorum, Yozgat, Kırıkkale, Nevşehir provinces in the Central Anatolia region of Turkey between January-2018 and December-2020. Bovines included in the study, simple random sampling method and 320 bovine blood serum and milk samples that were reported not to have Brucella vaccine were used. RBPT, SAT, CFT, I-ELISA tests were performed in the blood serum samples included in the study and I-Elisa, MRT tests were performed in the milk samples.
Result: The tests revealed that an average of 35.4% of the cattle had the bacteria while 64.5% of the raw milk samples were infected with brucellosis. The findings demonstrated the need to implement better strategies for managing and preventing brucellosis among animals, including vaccination. As a result, indirect ELISA (I-ELISA) method is considered as a useful, reliable, fast tool in the detection of Brucella from in milk and serum samples. However, it I-ELISA method can be used as a support for conventional tests in the initial isolation of waste materials and that its simultaneous use in field screening tests can be good diagnostic performance.

  1. Abbasoğlu, U., Mevsim, G., Günay, G. (1990). Brucella antibody screening in a rural district of Ankara. Journal of Turkish Microbiology Society. 20: 189-192.

  2. Acharya, K.P. Niroula, N. and Kaphle, K. (2016). Review of brucellosis in Nepal. Epidemiological Health. 38: e2016042. DOI: 10.4178/epih.e2017018.

  3. Alton G.G., Forsyth, J.R.L. (1996). Medical Microbiology. In: Medical Microbiology. [Samuel Baron, (editor)]. 4th ed. Galveston (TX): University of Texas Medical Branch at Galveston.

  4. Anonymous, (2009). Bovine Brucellosis. Eriþim: fileadmin/Home/fr/Health_standards/tahm/2.04.03_Bovıne_ Brucell.pdf. (Access Date: 03.05.2014).

  5. Arenas-Gamboa, A. M., Simmons, H.L., Krecek, R.C., Logan, L.L., Ellis, D., Ptaschinski, M. et al. (2021). AgSecure Africa ProgrammeTM: A Blended Training Approach for Africa. Journal of Veterinary Medical Education. e20200047.

  6. Babaoglu, U., Ogutucu, H., Demir, G., Sanli, D., Babaoglu, A. and Oymak, S. (2018). Prevalence of Brucella in raw milk: An example from Turkey. Nigerian Journal of Clinical Practice. 21: 907-911.  DOI:10.4103/njcp.njcp_211_17.

  7. Bosilkovski, M. (2019). Brucellosis: Treatment and Prevention. In Baron, E.L. (Ed.), Retrieved December 8, 2020, from

  8. Deka, R.P., Magnusson, U., Grace, D. and Lindahl, J. (2018). Bovine brucellosis: Prevalence, risk factors, economic cost and control options with particular reference to India- A review. Infection Ecology and Epidemiology. 8(1): 1-8.  DOI:10.1080/20008686.2018.1556548.

  9. Esendal, Ö.M., Yardımcı, H., Yıldırım, M., Altay, G (2000). The Use of Conventional Tests and Coombs Test in the Serological Diagnosis of Cattle, Sheep and Goat Brucellosis. IV. National Veterinary Microbiology Congress Book, Ankara p: 36.

  10. Facciolà, A., Palamara, M., D’Andrea, G., Marano, F., Magliarditi, D., Puglisi, G., Visalli, G. (2018). Brucellosis is a public health problem in southern Italy: Burden and epidemiological trend of human and animal disease. Journal of Infection and Public Health. 11: 861-866. DOI: 10.1016/j.jiph.2018.07.007.

  11. Gotuzzo, E., Celillo, E. (1992). Brucella. In: Infectious Diseases. [Gorbach, S.I., Bartlett, J.G., Blacklow, N.R., (eds)]. Harcourt Brace Jovanovich Inc Philadelphla. pp: 1513-1518.

  12. Khan, M.Z. and Zahoor, M. (2018). An overview of brucellosis in cattle and humans and its serological and molecular diagnosis in control strategies. Tropical Medicine and Infectious Diseases. 3: 65-79. DOI: 10.3390/tropicalmed 3020065.

  13. Kalkan, A., Felek, S., Akbulut, A., Papila, Ç., Demirdağ, K., Kılıç, S.S. (1999). Determination of brucellosis seroprevalence in various risk groups in Elazig region. Journal of Infection. 13: 227-230.

  14. Kumar, V.N., Bharathi, M.V. and Porteen, K. (2018). Evaluation of milk ring test and milk ELISA in diagnosis of bovine brucellosis. The Indian Veterinary Journal. 95: 78-79.

  15. Laborde, J., Sguazza, G., Fuentealba, N., Corva, S., Carbone, C., Galosi, C. (2017). Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies. Revista Argentina de Microbiologia. 49: 210-215. DOI:10.1016/j.ram.2017.02.005.

  16. Molavi, M., Sajjadi, H., Nejatizade, A. (2014). Effective methods for appropriate diagnosis of brucellosis in humans and animals (Review article). Online Journal of Animal and Feed Research. 4: 60-66.

  17. Yazıcıoğlu, N. (2012). Brusella Eradikasyon Programı. IV. Türkiye Zoonotik Hastalıklar Sempozyumu (Gıda Kaynaklı Zoonozlar), Sempozyum kitabı, 99-106.

  18. Young, E.J. (2000). Brucella Species. In: Principles and Practice of Infectious Diseases. [(Eds.) Mandell, G.L., Bennett, J., Dolin, R.]. 5th ed., New York, Chuchill Livingstone pp: 2386-2391.

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