Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 55 issue 12 (december 2021) : 1430-1438

A Comprehensive Description and Evolutionary Analysis of Testudines Mitochondrial Genomes

Handong Wang, Ye Chen, Wei Shi, Yongyao Guo, Jinghong He, Zhangjie Chu, Bo Zhao
1College of Fisheries, Zhejiang Ocean University, No. 1, Haida South Road, Lincheng Street, Zhoushan, Zhejiang Province, China.
Cite article:- Wang Handong, Chen Ye, Shi Wei, Guo Yongyao, He Jinghong, Chu Zhangjie, Zhao Bo (2021). A Comprehensive Description and Evolutionary Analysis of Testudines Mitochondrial Genomes. Indian Journal of Animal Research. 55(12): 1430-1438. doi: 10.18805/IJAR.B-1394.
Background: There are not many species of turtles and some species have become rare or even endangered due to the changes in the ecological environment, the destruction of human pet market trade, the use of food and medicinal materials and other factors. The phylogenetic study of Geoemyda spengleri and their related species will help to protect turtle germplasm resources. 
Methods: The sample was collected from nature reserves in Guangxi, China and processed for DNA isolation and confirmed with Polymerase chain reaction (PCR). Maximum-likelihood (ML) were conducted based on concatenated sequences of 13 protein-coding genes from mitochondrial genomes of 25 taxa.
Result: The complete mitochondrial genome (17,448 bp) from the Black-breasted leaf turtle (Geoemyda spengleri) was determined. The genome content, gene order and base composition conformed to the consensus vertebrate type mtDNA. However, a remarkable feature was found in this molecule: a small number of (ATATTATTATATTATTATATATC)n direct tandem repeats followed by a AT-enriched microsatellite sequence at the 3’ end of the control region (D-loop), which might be useful as molecular markers for studying population genetics and helpful for species identification and conservation. The results strongly supported that 1) Geoemyda spengleri and the most recent common ancestor of Batagur trivittata and Pangshura sylhetensis formed a monophyletic clade, whereas most other species of Geoemydidae formed another branch, suggesting that Geoemyda and Batagur trivittata may have more closely relationships than other genera; 2) the Geoemydidae with Testudinidae was a sister group rather than with the Emydidae. Furthermore, In order to analyze the relationship between habitat distribution and the phylogenetic evolution of turtles, the habitat distribution map was plotted based on the habitat distribution of species of Geoemydidae. The results also supported that Geoemyda spengleri and Batagur trivittata may relatively have intimate relationships.

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