Loading...

​Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs with White Muscle Disease

DOI: 10.18805/IJAR.B-1372    | Article Id: B-1372 | Page : 1293-1297
Citation :- ​Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs with White Muscle Disease.Indian Journal of Animal Research.2021.(55):1293-1297
Kıvanç Irak, Handan Mert, Nihat Mert, Nesrullah Ayşin, İnci Doğan Söğütlü, Arzu Comba kivancirak@hotmail.com
Address : Department of Biochemistry, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey.
Submitted Date : 9-04-2021
Accepted Date : 1-07-2021

Abstract

Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. 
Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined.
Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups.

Keywords

​Blood parameters Glutathione Liver Malondialdehyde Thyroid White muscle disease


References

  1. Abbas, S., Iqbal, R., Butt, M.Z., Niaz, S., AbdEl-Salam, N.M., Haleem, S. and Ullah, R. (2019). Effects of selenium and Vitamin E on liver function and food conversion ratio in Rhode Island Red. Indian Journal of Animal Research. 53(12): 1589-1593.
  2. Ateşşahin, A., Pirinçci, İ., Gürsu, F. and Çikim, G. (2002). Effects of selenium on thyroid hormone levels in sheep. Turkish Journal of Veterinary and Animal Sciences. 26(6): 1401-1404. 
  3. Berry, M. J., Banu, L. and Larsen, P.R. (1991). Type I iodothyronine deiodinase is a selenocysteine-containing enzyme. Nature. 349(6308): 438-440.
  4. Beutler, E. (1963). Improved method for the determination of blood glutathione. The Journal of Laboratory and Clinical Medicine. 61: 882-888.
  5. Beytut, E., Erişir, M. and Aksakal, M. (2001). Reduced glutathione and malondialdehyde levels with catalase enzyme activity in the heart, skeletal muscle and liver of lambs with white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 7(1): 1-5.
  6. Beytut, E., Karatas, F. and Beytut, E. (2002). Lambs with white muscle disease and selenium content of soil and meadow hay in the region of Kars, Turkey. Veterinary Journal. 163(2): 214-217.
  7. Bildik, A., Yur, F., Çamaş, H., Dede, S. and Sekin, S. (1996). Investigation of Glutathione and Lipid Peroxidation Levels with Respect to Haemoglobin Types in Lambs with White Muscle Disease. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 7(1): 95-98.
  8. Broderius, M. A., Whanger, P. D. and Weswig, P. H. (1973). Tissue Sulfhydryl Groups in Selenium-deficient Rats and Lambs. The Journal of Nutrition, 103(3): 336-341.
  9. Çamaş, H., Ertürk, K. and Ersoy, E. (1976). Normal ve muskuler distrofili kuzuların kan serumlarında total protein, protein fraksiyonları, kreatinin, kreatin fosfokinaz, glutamik piruvat transaminaz ve glutamik okzalasetik transaminaz yönünden araþtırmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 23: 249-259.
  10. Değer, Y., Mert, H., Mert, N., Yur, F., Kozat, S., Yörük, İ.H. and Sel, T. (2008). Serum selenium, vitamin E and sialic acids concentrations in lambs with white muscle disease. Biological Trace Element Research. 121(1): 39-43.
  11. Elsheikh, A.H., Al-Hassan, M.J., Mohamed, H.E. and Abudabos, A.M. (2014). Effect of injectable sodium selenite on the level of stress biomarkers in male aardi goats. Indian Journal of Animal Research. 48(3): 239-243.
  12. Fernandez, V. and Videla, L.A. (1981). Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of various tissues of the rat. Experientia. 37(4): 392- 394.
  13. Giray, B., Riondel, J., Richard, M. J., Favier, A. and Hıncal, F. (2004). Oxidant/Antioxidant status in relation to thyroid hormone metabolism in selenium and/or iodine deficient rats. The Journal of Trace Elements in Experimental Medicine: The Official Publication of the International Society for Trace Element Research in Humans, 17(2): 109-121.
  14. Hulland, T.J. (1985). Muscles and Tendons in: KVF Jubb, PC Kennedy, N Palmer (Eds.), Pathology of Domestic Animals, 1, Academic Press Inc, London, p. 140-195. 
  15. İmren, H.Y. and Şahal, M. (1991). Veteriner İç Hastalıkları Kitabı, Feryal Yayıncılık, Ankara. 
  16. Jensen, L. S., Colnago, G. L., Takahashi, K. and Akiba, Y. (1986). Dietary selenium status and plasma thyroid hormones in chicks. Biological trace element research, 10(1): 11-18.
  17. Karapınar, T. and Yılmaz, S. (2007). Investigation of white muscle disease in cattle. Fırat Üniversitesi Doðu Araþtırmaları Dergisi, 6(1): 100-102.
  18. Kozat, S., Gunduz, H., Deger, Y., Mert, N., Yoruk, I.H. and Sel, T. (2007). Studies on serum alpha-tocopherol, selenium levels and catalase activities in lambs with white muscle disease. Bulletin-Veterinary Institute in Pulawy. 51(2): 281-284.
  19. Maia, A.L., Goemann, I.M., Souza Meyer, E.L. and Wajner, S.M. (2011). Type 1 iodothyronine deiodinase in human physiology and disease. Journal of Endocrinology, 209(3): 283-297.
  20. Mcdowell, L.R. (1992). Selenium Minerals in Animal and Human Nutrition, Academic Press Inc, London, 294-332. 
  21. Mert, H., Yıldırım, S., Yörük, İ.H., Irak, K., Comba, B., Mert, N., Ayşin, N. and Comba, A. (2018). Retinol, α-tocopherol and vitamin D3 in White Muscle disease. Medycyna Weterynaryjna, 74 (7): 441-444.
  22. Mert, N. (1996). Veterinary Clinical Biochemistry, Ceylan Typography Ltd., Bursa.
  23. Miller, J.K., Brzezinska-Slebodzisnka, E. and Madsen F.C. (1993). Oxidative stress, antioxidants and animal function. Journal of Dairy Science, 76(9): 2812-2823.
  24. Naziroğlu, M., Mehmet, Ç. A. Y., Tahan, V., Ramazan, B. A. L. and Delibaş, N. (1998). Effects of selenium and vitamin E supplementation on concentrations of plasma thyroid hormones in lambs. Turkish Journal of Veterinary and Animal Sciences. 22(2): 157-160.
  25. Nolan, M. R., Kennedy, D. G., Blanchflower, W. J. and Kennedy, S. (1995). Feeding corn oil to vitamin E-deficient pigs increases lipid peroxidation and decreases tissue glutathione concentrations. International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin-und Ernahrungsforschung. Journal International de vitaminologie et de Nutrition. 65(3): 181-186.
  26. Or, M.E., Dodurka, H.T. and Kayar, A. (2003). The diagnostic importance of erythrocyte glutathione peroxidase (GSH- Px) activity and some serum parameters in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences. 27(1): 1-6.
  27. Osame, S., Ohtani, T. and Ichijo, S. (1990). Studies on serum tocopherol and selenium levels and blood glutathione peroxidase activities in lambs with white muscle disease. The Japanese Journal of Veterinary Science. 52(4): 705-710.
  28. Ozdemir, O., Ciftçi, M.K., Hatipoglu, F., Ortatatli, M., Yavuz, O., Kanat, O. (2016). Nutritional cardiomyopathy in a young camel (C. dromedarius). Eurasian Journal of Veterinary Sciences. 32(1): 52-54.
  29. Pamukçu, T., Sel, T. and Yarim, G. (2001). Blood serum concentrations of selenium and glutathione peroxidase activity in Akkaraman sheep. Turkish Journal of Veterinary and Animal Sciences. 25(5): 731-734.
  30. Rizzo, A. F., Atroshi, F., Ahotupa, M., Sankari, S. and Elovaara, E. (1994). Protective effect of antioxidants against free radical mediated lipid peroxidation induced by DON or T 2 toxin. Journal of Veterinary Medicine Series A. 41(110): 81-90.
  31. Turgut, K. (1995). Veteriner Klinik Laboratuvar Teshis, Konya.
  32. Van Metre, D.C. (2001). Selenium and vitamin E. Veterinary Clinics of North America: Food Animals Practice. 17(2): 373- 402.
  33. Yavuzer, U. and Bengisu, G. (2014). Effect of selenium on the reproductive performance of ewes and white muscle disease in lambs. Indian Journal of Animal Research. 48(3): 248-250.
  34. Xia, E., Rao, G., Van Remmen, H., Heydari, A.R. and Richardson, A. (1995). Activities of antioxidant enzymes in various tissues of male Fischer 344 rats are altered by food restriction. The Journal of Nutrition. 125(2): 195-201.

Global Footprints