The Impact of Breed, Testing Time and Metabolic Profile on the Variation of Copper Concentration in Sheep Blood Serum

DOI: 10.18805/IJAR.B-1352    | Article Id: B-1352 | Page : 767-773
Citation :- The Impact of Breed, Testing Time and Metabolic Profile on the Variation of Copper Concentration in Sheep Blood Serum.Indian Journal of Animal Research.2021.(55):767-773
J. Autukaitė, V. Juozaitienė, R. Antanaitis, I. Poškienė, W. Baumgartner, H. Žilinskas, V. Žilaitis jurgita.autukaite@lsmuni.lt
Address : Veterinary Academy, Lithuanian University of Health Sciences, Tilžės str. 18, Kaunas, Lithuania.
Submitted Date : 15-02-2021
Accepted Date : 1-05-2021


Background: Copper supplementation in ruminants deserves special attention because of the narrow margin between deficiency and toxicity. The aim of the study was to analyse the relationship between the Cu concentration and other blood parameter values having taken into consideration their physiological limits based on the variation of copper concentration in the three different sheep breeds. 
Methods: Sixty sheep (35±5 kg) kept on the same farm were studied: the Suffolk (n = 20), the Merino (n = 20) and the Lithuanian blackhead (n = 20). The sheep were selected according to the following parameters: 3.5±0.3 years old, not pregnant during the entire investigation period, clinically healthy. All sheep were kept under the same conditions and throughout the year, were fed at the same time the same ration balanced according to their physiological needs. Individual blood samples were collected on the same day, on monthly basis for the period of one year (April 2018 to May 2019). Blood biochemical parameters as well blood haematology and cortisol concentration were analysed.
Result: The highest average value of copper concentration was found in the Merino breed. The study has shown that the lowest number of blood samples with the levels of copper meeting the physiological limit values was found in the native Lithuanian blackhead sheep at the end of the grazing period and in the Merino and the Suffolk breeds - during the coldest winter months. The increase in copper concentration could be related to the stress caused due to the changes in dietary conditions, the variations of ambient temperature and different breed response to these factors.


Biochemical parameters Breed Copper Haematology Sheep


  1. Adams, S., Hongjiao, M., Che, D., Hailong, J., Rui, H., Bao, Z., Danquah, K. and Guixin, Q. (2018). Effect of dietary copper levels on the growth performance and nutrientutilization in fattening pigs. Indian Journal of Animal Research. 54(5): 573-577.
  2. Anchordoquy, J.M., Anchordoquy, J.P., Nikoloff, N., Pascua, A.M. and Furnus, C.C. (2017). High copper concentration produce genotoxicity and cytotoxicity in bovine cumulus cells. Environmental Science and Pollution Research. 24: 20041-20049.
  3. Arfuso, F., Fazio, F., Rizzo, M., Marafioti, S., Zanghì, E. and Piccione, G. (2016). Factors affecting the hematological parameters in different goat breeds from Italy. Annals of Animal Science. 16: 743-757.
  4. Behera, R., Chakravarty, A.K., Sahu, A., Kashyap, N., Rai, S. and Mandal, A. (2018). Identification of best temperature humidity index model for assessing impactof heat stress on milk constituent traits in Murrah buffaloes under subtropical climatic conditions of Northern India. Indian Journal of Animal Research. 52: 13-19.
  5. Cybulski, W., Jarosz, L., Chalabis-Mazurek, A., Jakubczak, A., Kostro, K. and Kursa, K. (2009). Contents of zinc, copper, chromium and manganese in silver foxes according to their age and mineral supplementation. Polish Journal of Veterinary Science.12: 339-345.
  6. Durak, M.H., Erkan, R.E.C., Celik, R., Yokus, B., Kurt, D. and Gürgöze, S. (2015). The effects of age and gender on some biochemical serum parameters in zom sheep raised in the vicinity of Karacadað. Israel Journal of Veterinary Medicine. 70: 33-39.
  7. Higgs, P., Costa, M., Freke, A. and Papasouliotis, K. (2014). Measurement of thyroxine and cortisolin canine and feline blood samples using two immunoassay analysers. Journal of Small Animal Practice. 55: 153-159.
  8. Kramer, J.W. (2000). Normal hematology of cattle, sheep and goats. In: A Textbook of Schalm’s Veterinary Hemattology. 5th Edn, Philadelphia, Lippincott Williams and Wilkins. pp. 1075-1084.
  9. Kulkarni, R.C., Shrivastava, H.P. and Mandaland A.B. (2012). Effect of copper supplementation on serum trace mineral status and cholesterol in broiler chickens. Indian Journal of Animal Research. 3: 313-314.
  10. López-Alonso, M. and Miranda, M. (2020). Copper supplementation, a challenge in cattle. Animals. 10: 1890.
  11. Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. (2000). In: A Textbook of the biochemical values for cattle, sheep and pigs. 9th Edn, London. pp. 1819-1822.
  12. Ramirez, R.G., Haenlein, G.F. and Nunez-Gonzalez, M.A. (2001). Seasonal variation of macro and trace mineral contents in 14 browse species that grow in northeastern Mexico. Journal Small Ruminant Research. 39: 153-159.
  13. Shen, X.Y., Li, X. and Zhang, R.D. (2010). Studies on unsteady gait disease of the Tibetan gazelles. Journal of Wildlife Disease. 46: 560.
  14. Stevanović. O., Stojiljković, M., Nedić. D., Radoja, D., Nikolić, V., Prodanović, R., Ivanov, S. and Vujanac, I. (2015). Variability of blood serum biochemical parameters in karakachan sheep. Journal Biotechnology and Animal Husbandry. 31: 55-62.
  15. Suttle, N.F., Lewis, R.M. and Small, J.N.W. (2002). Effects of breed and family on rate of copper accretion in the liver of purebred Charollais, Suffolk and Texel lambs. Journal of Animal Science. 75: 295-302. 
  16. Yokus, B., Cakir, D.U., Kanay, Z., Gulten, T. and Uysal, E. (2006). Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. Journal of Veterinary Medicine. 53: 271-276. 

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