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The Effects of Negative Pressure on the Gene Expression of Motility Related Proteins in Boar Spermatozoa during Liquid Storage at 17oC

DOI: 10.18805/IJAR.B-1337    | Article Id: B-1337 | Page : 511-516
Citation :- The Effects of Negative Pressure on the Gene Expression of Motility Related Proteins in Boar Spermatozoa during Liquid Storage at 17oC.Indian Journal of Animal Research.2021.(55):511-516
Yanbing Li, Jingchun Li, Qun Zhang, Qian Wang, Minghui Guo, Qi Li, Guosheng Wei elj863@163.com
Address : College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
Submitted Date : 30-10-2020
Accepted Date : 28-01-2021

Abstract

Background: The present study was aimed to investigate the effects of negative pressure applied before storage on the gene expression of motility related proteins in boar spermatozoa.
Methods: Boar semen samples were collected and pooled and diluted with Modena solution containing 0.4% (w/v) of bovine serum albumin. Negative pressure was applied for 2-5 min using a vacuum pump with a barometer. The pressure applied was 0 (Control), -0.02 MPa (P2), -0.04 MPa (P4) and -0.08 MPa (P8). The expression of AQN-1, AQN-3, AWN, PSP-I, PSP-II gene in boar spermatozoa was evaluated.
Result: Application of -0.04 MPa improved the sperm motility compared with the other groups. In conclusion, our results confirmed thatnegative pressure preservation at 17oC had an effect on the expression of boar sperm adhesion protein gene. The relative expression of AWN0PSP-I and PSP-II genes in boar sperm were lower under -0.04 MPa and -0.08 MPa pressure, which was beneficial to protect spermatozoa motility. 

Keywords

Boar semen Gene expression Motility related proteins Negative pressure

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