Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 57 issue 2 (february 2023) : 254-257

Studies on Bioaccumulation of Lead and Arsenic in Different Tissues of Rabbit (Oryctolagus cuniculus)

Ahmad Ali1, Muhammad Zubair Hussain2,*, Sheikh Muhammad Azam3, Ghulam Mustafa4, Gulnaz Afzal1, Mussadiq Idrees5, Khalid Javed Iqbal1
1Department of Zoology, Islamia University, Bahawal Pur, Pakistan.
2Department of Zoology, Government Emerson College, Multan, Pakistan.
3Department of Zoology, University of Education Lahore, Dera Ghazi khan Campus, Pakistan.
4Department of Zoology, University of Veterinary and Animal Sciences, Lahore.
5Department of Physiology, Islamia University, Bahawal Pur, Pakistan.
Cite article:- Ali Ahmad, Hussain Zubair Muhammad, Azam Muhammad Sheikh, Mustafa Ghulam, Afzal Gulnaz, Idrees Mussadiq, Iqbal Javed Khalid (2023). Studies on Bioaccumulation of Lead and Arsenic in Different Tissues of Rabbit (Oryctolagus cuniculus) . Indian Journal of Animal Research. 57(2): 254-257. doi: 10.18805/IJAR.B-1334.
Heavy metals present in surroundings tend to accumulate into the bodies of animals vide the food chains. This bioaccumulation of toxic heavy metals cause several pathological conditions, thus, imposing serious health hazards to humans and other animals. It has become extremely important to monitor levels of heavy metals for well being of humans. The present study was carried to evaluate the extent of bioaccumulation of two heavy metals in rabbit by measuring their levels in various tissues. The rabbits were divided into control (C) and two experimental groups i.e. T1 (Lead treated) and T2 (Arsenic treated). Experimental groups were orally administered lead and arsenic at concentration of 0.02 mg/L of glucose solution for a period of 28 days. Further, the concentration of above heavy metals was determined in liver, kidney and muscle using atomic absorption spectrometry. Concentration of lead in liver and kidney, while concentration of arsenic in kidney was found to be significantly higher (P≤0.05) in treatment groups as compared to the control. Higher mean concentration of lead (35.68±7.36) and arsenic (18.70±3.456) was detected in kidney in treatment groups. Lower mean concentration of lead (12.43±4.70) and arsenic (7.07±2.45) was determined in muscles in treatment groups. The lead accumulated at significantly higher rate (P≤0.05) compared to arsenic in all three tissues in treatment groups. It is concluded that heavy metals tend to bioaccumulate at relatively higher concentration in tissues involved in metabolic activities i.e. kidney and liver in rabbit.
Toxicity of heavy metals is a problem of increasing significance due to their wide ranging effects observed from environmental, evolutionary as well as nutritional perspective (Nagajyoti et al., 2010; Jaishankar et al., 2014). Health hazards associated with heavy metals are due to their tendency to bioaccumulation i.e. their concentration increases in an organism over time, compared to their concentration in the environment (Kamrin, 1997). The heavy metals can enter in an animal’s body via air, drinking water and food. Several of the heavy metals including arsenic, lead, cadmium, copper, nickel etc are commonly found in waste water and cause risks for human health and the environment (Lambert et al., 2000). Excessive accumulation of heavy metals in the human body through dietary intakes causes serious health problems (Oliver, 2007).

The risk associated with the exposure to heavy metals present in food products has aroused widespread concern about human health. Biomonitoring of the heavy metals concentrations in food chains is extremely important for the wellbeing of all organisms. Exposure of heavy metals to humans in normal populations as well as in occupational circumstances has created an increasing interest in biomonitoring of these heavy metals (Drasch et al., 1997). The present study aimed to assess the extent of bioaccumulation of two heavy metals i.e. lead and arsenic in various tissues of a model organism i.e. the rabbit (Oryctolagus cuniculus), under laboratory conditions.
The current study was carried out during May 2016 to June 2016, at the Animal House, Department of Life Science, Baghdad Al Jadeed campus, Islamia University Bahawal Pur, Pakistan. A total of fifteen number of rabbits (Oryctolagus cuniculus) belonging to the local strain were divided into three groups (N=5 in each group) i.e. control (C), lead treatment group (T1) and arsenic treatment group (T2). T1 and T2 groups were administered lead and arsenic orally respectively at concentration of 0.02 mg/L in glucose, while the control group was given saline in glucose only as treatment. At the end of experiment, the animals were euthanized and dissected to collect the tissue samples of liver, kidney and muscles under aseptic conditions. Tissues were washed with double distilled water, kept in labeled plastic bottles and stored at -20oC till further analysis.

HNO3 and H2SO4 were taken in 2:1 ratio and mixed to prepare the aqua regia. One gram of sample was mixed with aqua regia for approximately 16 hours for metal digestion. The obtained suspension was heated at 130°C for 2 hours. The resulting solution was filtered using Whatman’s filter paper. The filterate was diluted by adding HNO3 (0.5 mole per litre) making final volume upto 100 mL. The prepared samples were then preserved at 4°C for heavy metal estimation (Kishe and Machiwa, 2003; Woitke et al., 2003). Concentrations of metals in the digested samples were determined with a flame/flameless atomic absorption spectrophotometer (AAS, Z-2010, Hitachi High-Technologies, Japan) equipped with a Zeeman graphite furnace, at Fish quality Control Laboratoty (Fisheries Research and Training Institute, Batapur Manawan) Lahore, Pakistan. The instrument was calibrated using standard solutions of the respective metals to establish standard curves before metal analysis.
Statistical analysis
The concentrations of the two heavy metals in various tissues of treatment groups of rabbit were compared with control group by using t-test.
Mean values of lead concentrations were 35.68±7.368 ppb in kidneys, 27.95±6.385 ppb in liver and 12.43±4.702 ppb in muscles in animals of treated group. Similarly, the average lead levels were 7.49±1.49 ppb in liver, 7.05±0.69 ppb in kidney and 0.45±0.19 ppb in muscles in animals of control group. Maximum concentration of lead was accumulated in kidney (40.89 ppb) followed by liver (32.46 ppb) and muscles (15.76 ppb) in treatment group, whereas, the maximum concentration of lead was found in liver (8.54 ppb) followed by kidney (7.54 ppb) and muscles (0.59 ppb) in control group (Table 1). The lead accumulation was significantly higher (P≤0.05) in liver and kidneys of treatment group compared to the control.

Table 1: Concentration (ppb) of heavy metals (arsenic and lead) in various tissues of rabbit (Oryctolagus cuniculus).

Mean values of arsenic concentration was 18.70±3.45 ppb in kidneys, 18.01±3.76 ppb in liver and 7.07±2.45 ppb in muscles in animals of treated group. Similarly, the average arsenic levels were 4.21±0.80 in kidney, 2.77±0.62 ppb in liver, while not detected in muscle tissue of control group. Maximum concentration of arsenic was accumulated in kidney (23.64 ppb) followed by liver (21.43 ppb) and muscles (10.23 ppb) in treatment group, whereas, the maximum concentration of arsenic was found to be in kidney (4.78 ppb) followed by liver (3.21 ppb) and below detection limits in muscle (Table 1). The arsenic accumulation was significantly higher (P≤0.05) in kidneys of treatment group compared to the control.

In the present study, it was found that the bioaccumulation of two heavy metals i.e. arsenic and lead increased several times in treated animals compared to control animals. Further the concentrations of these heavy metals detected in control group were within the recommended limits. Guideline values recommended for lead and arsenic in drinking water are 0.05 mg/L and 0.005 mg/L respectively (WHO, 1996). There are no published data concerning the heavy metals level in tissues of rabbits (Oryctolagus cuniculus) from Pakistan.

High concentrations of heavy metals have been detected in mammals inhabiting polluted areas (Ma et al., 1991; Świergosz-Kowalewska et al., 2005; Sanchez-Chardi et al., 2009). Concentration of heavy metals including lead has been measured in liver and muscles of a rodent (Ctenomys talarum) in areas with different amount of exposure to pollution (Schleich et al., 2010). The major path of heavy metal exposure in animals is oral consumption (Baker et al., 2003). Heavy metals then accumulate into different organs of the body (kidney, liver and muscles). Significant levels of lead accumulation have been recorded from muscle tissues of a freshwater and marine fish (Ahmed et al., 2016; Anjum et al., 2019). Absorbed lead is stored in soft tissues mainly the liver tissues (Lyn-Patrick, 2006). In a previous study, lead accumulated at different rates in various tissues of hare (Lepus nigricollis). The higher concentration was found in kidney and liver while lower concentration was found in muscles (Shahid et al., 2013). Results of our study coincide with the results of this study.

The lead concentrations in muscle of rabbits in the present study was more than the values recorded in muscles of ruminants in several other studies (Falandysz, 1993; Tahvonen and Kumpulainen, 1994; Doganoc, 1996). Higher concentration of lead has been recorded in kidney than in liver (Venalainen et al., 1996). This was consistent with the findings of our study.

In one study conducted in goats, arsenic was detected at elevated levels in several tissues with significant increase in kidney and liver (Vahter and Marafanate, 1987). The maximum concentration was found in liver of goat. Our results were partly in agreement with the reports of Vahter and Marafanate (1987) in goat, as we found significantly higher concentrations of arsenic in liver and kidney of rabbit. However, the maximum concentration was found in kidney of rabbit though concentration level of arsenic in kidney and liver of rabbit differed only in decimals (Table 1). The mean concentrations of arsenic detected in liver and kidney in rabbits in present study are also in agreement with another study conducted in sheep and goat (Akoto et al., 2014). Our study shows greater mean value of arsenic in kidney, liver and muscles compared to work of Alonso et al., (2000) who detected concentrations of three toxic elements i.e. arsenic, cadmium, lead in several tissues of liver, kidney, muscle and blood of calves and cows. Whereas, arsenic concentrations in liver, kidney and muscle from cattle in Sweden (Jorhem et al., 1991) and Australia (Kramer et al., 1983) were lower than those in the present study. These differences in accumulated concentrations may be due to differences of metals in environment.

Our study revealed that the kidney and liver were the most vulnerable organs to chronic arsenic exposure which was similar to the finding of Al-forkan et al., (2016). These findings along with several previous studies (Abou-Arab, 2001; Hussein et al., 2013) demonstrated that the liver and kidneys were the target tissues for monitoring metal contamination in animals. Both organs played key role in removing the toxic metals from the body and therefore ended up accumulating them. It could be concluded that the heavy metals tend to bioaccumulate at relatively higher concentration in tissues with key role in metabolism of toxic substances i.e. kidney and liver in rabbit.

  1. Ahmed, Q., Benzer, S. and Yousuf, F. (2016). Distribution of heavy metals in different tissues of Indian mackerel from Karachi fish harbour, Karachi, Pakistan. Indian Journal of Animal Research. 50: 759-763. 

  2. Anjum, K.M., Yaqub, A., Bhatti, E.M., Yameen, M., Mughal, M.S., Khan, N., Rasool, F., Abbas, S. and Waseem Aslam, W. (2018). Assessment of zinc and lead concentration in water and muscles of Labeo rohita collected from wild and local fish farms of Pakistan. Indian Journal of Animal Research. 52: 1560-1564.

  3. Abou-arab, A.A.K. (2001). Heavy metal contents in Egyptian meat and the role of detergent washing on their levels. Food and Chemical Toxicology. 39(6): 593-599.

  4. Akoto, O., Sam, N.B., Shouta, M.M., Nakayama., Ikenaka, Y., Baidoo, E., Yohanne, S., Hazuki ,Y.B., Mizukaw, A. and Ishizuqa, M. (2014). Distribution of heavy metals in organs of sheep and goat reared in obuasi: A gold mining town in Ghana. International Journal of Environmental Science and Toxicology Research. 2(4): 81-89.

  5. Al-forkan, M., Islam, S., Akter, R., Shameen-alam, S. and Khaleda, L. (2016). A sub-chronic exposure study of arsenic on hematological parameters, liver enzyme activities, histological studies and accumulation pattern of arsenic in organs of wistar albino rats. Journal of Cytologyand Histotology. S5: 06: 2-7.

  6. Alonso, M.L., Benedito, J.L., Miranda, M., Castillo, C., Hernandez, J. and Shore, R.F. (2000). Arsenic, cadmium, lead, copper and zinc in cattle from Galicia, NW Spain. Science of the Total Environment. 246(2): 237-248.

  7. Baker, S., Herrchen, M., Hund-rinke, K., Klein, W., Kordel, W., Peijnenburg, W. and Rensing, C. (2003). Underlying issues including approaches and information needs in risk assessment. Ecotoxicology and Environmental Safety. 56(1): 6-19.

  8. Doganoc, D.Z. (1996). Lead and cadmium concentrations in meat, liver and kidney of Slovenian cattle and pigs from 1989 to 1993. Food Additives and Contaminants. 13: 237-241.

  9. Drasch, G., Wanghofer, E. and Roider, G. (1997). Are blood, urine, hair and muscle valid bio-monitoring parameters for the internal burden of men with the heavy metals mercury, lead and cadmium? Trace Elements and Electrolytes. 14: 116-123.

  10. Falandysz, J. (1993). Some toxic and essential trace metals in cattle from the northern part of Poland. Science of the Total Environntme 136: 177-191.

  11. Hussein, H.K., Abu-zinadah, O.A., El-rabey, H.A. and Meerasahib, M.F. (2013). Estimation of some heavy metals in polluted well water and mercury accumulation in broiler organs. Brazilian Archives of Biology and Technology. 56(5): 767-776.

  12. Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B.B. and Beeregowda, K.N. (2014). Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary toxicology. 7(2): 60-72.

  13. Jorhem, L., Slorach, S., Sundström, B. and Ohlin, B. (1991). Lead, cadmium, arsenic and mercury in meat, liver and kidney of Swedish pigs and cattle in 1984-88. Food Additives and Contaminants. 8(2): 201-211.

  14. Kamrin, M.A. (1997). Pesticide Profiles: Toxicity, Environmental Impact and Fate. Boca Raton, FL CRC/Lewis Publishers.

  15. Kishe, M.A. and Machiwa, J.F. (2003). Distribution of heavy metals in sediments of Mwanza Gulf of Lake Victoria, Tanzania. Environment International. 28(7): 619-625. 

  16. Kramer, H.L., Steiner, J.W. and Vallely, P.J. (1983). Trace element concentration in the liver, kidney and muscle of Queensland cattle. Bulletin of Environmental Contaminants and Toxicology. 30: 588- 594.

  17. Lambert, M., Leven, B.A. and Green, R.M. (2000). New Methods of Cleaning up Heavy Metal in Soils and Water; Environmental Science and Technology Briefs for Citizens; Manhattan, KS: Kansas State University.

  18. Lyn-patrick, N.D. (2006). Lead toxicity, a review of the literature. Part 1: Exposure, evaluation and treatment. Alternative Medicine Review. 11(1): 2-22.

  19. Ma, W.C., Denneman, W. and Faber, J. (1991). Hazardous exposure of ground living small mammals to cadmium and lead in contaminated terrestrial ecosystems. Archives of Environmental Contaminants and Toxicology. 20: 266-270.

  20. Nagajyoti, P.C., Lee, K.D. and Sreekanth, T.V.M. (2010). Heavy metals, occurrence and toxicity for plants: A review. Environmental Chemistry Letters. 8: 199.

  21. Oliver, M.A. (2007). Soil and human health: A review. European Journal of Soil Science. 48: 573-92.

  22. Sanchez-chardi, A., Ribeiro, C.A.O. and Nadal, J. (2009). Metals in liver and kidneys and the effects of chronic exposure to pyrite mine pollution in the shrew Crocidura russula inhabiting the protected wetland of Doñana. Chemosphere. 76(3): 387-394.

  23. Schleich, C.E., Beltrame, M.O. and Antenucci, C.D. (2010). Heavy metals accumulation in the subterranean rodent Ctenomys talarum (Rodentia: Ctenomyidae) from areas with different risk of contamination. Folia Zoologica. 59(2): 108.

  24. Shahid, N., Anwar, S., Qadir, A., Ali, S., Suchentrunk, F. and Arshad, H.M. (2013). Accumulation of some selected heavy metals in Lepus nigricollis from Pakistan. Journbal of  Basic and Applied Scientific Research. 3(11): 339-346.

  25. Œwiergosz-kowalewska, R., Gramatyka, M. and Reczyñski, W. (2005). Metals distribution and interactions in tissues of shrews (Sorex spp.) from copper- and zinc-contaminated areas in Poland. Journal of Environmental Quality. 34: 1519-1529. 

  26. Tahvonen, R. and Kumpulainen, J.6 (1994). Lead and cadmium contents in pork, beef and chicken and in pig and cow liver in Finland during 1991. Food Additives and Contaminants. 11: 415-426.

  27. Vahter, M. and Marafante, E. (1987). Effects of low dietry intake of methionine, choline or proteins on the biotransformation of arsenite in the rabbit. Toxicology Letters. 37: 41-46.

  28. Venalainen, E.R., Niemi, A. and Hirvi, T. (1996). Heavy metals in tissues of hares in Finland, 1980-82 and 1992-93. Bulletin of Environmental Contaminants and Toxicology. 56: 252-258.

  29. WHO. (1996). World Health Organization: Health Criteria and other Supporting Information. In: Guideline for Drinking Water Quality. 2: 31-33.

  30. Woitke, P., Wellmit, Z.J., Helm, D., Kube, P., Lepom, P. and Litheraty, P. (2003). Analysis and assessment of heavy metal pollution in suspended solids and sediments of the river Danube. Chemosphere. 51(8): 633-642.

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