Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 55 issue 6 (june 2021) : 629-635

Isolation and Phylogenetic Analysis of the Canine Distemper Virus from a Naturally Infected Dog in China

Hongliang Zhang, Peipei Meng, Xiaoming Song, Shuguang Li, Ruimei Yang, Chuanmei Zhang, Hu Shan, Yongjun Wen
1Ministry of Agriculture Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Diseases, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot-010 018, China. 
Cite article:- Zhang Hongliang, Meng Peipei, Song Xiaoming, Li Shuguang, Yang Ruimei, Zhang Chuanmei, Shan Hu, Wen Yongjun (2020). Isolation and Phylogenetic Analysis of the Canine Distemper Virus from a Naturally Infected Dog in China. Indian Journal of Animal Research. 55(6): 629-635. doi: 10.18805/IJAR.B-1298.
Background: Canine distemper virus (CDV) can infect a variety of predators, such as Canidae, Mustelidae and Procyonidae family and cause high morbidity and mortality. Genetic/antigenic heterogeneity has been observed among various CDV strains, notably in the haemagglutinin (H) gene. Based on sequence analysis of the H gene, wild-type CDV strains can be divided into distinct genotypes. 
Methods: We used Vero/SLAM cells to isolate CDV strains from clinically naturally infected dog tissues. Here, the H gene sequence of the isolated strain was analyzed for the restriction enzyme polymorphism with RT-PCR-RFLP method and the method could be used to distinguish CDV vaccine strains from wild-type strains. Furthermore, the genetic and antigenic variation of the H gene was also analyzed.
Result: Our results indicated that Vero cells expressing canine SLAM allow the efficient isolation of CDV wild-type strains. The isolated CDV was named Shandong strain. Phylogenetic analysis identified that Shandong strain was CDV wild-type strain, the Asian genotype I. Analysis of the potential glycosylation site of the H gene and determination of the neutralization titer PD50 showed that there were immunogenic differences between the Shandong strain and the vaccine strains.
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