Loading...

The Differential Expressions of PPARγ and CAST mRNA in Muscle Tissues of Jinhua and Landrace Pigs

DOI: 10.18805/ijar.B-1272    | Article Id: B-1272 | Page : 536-541
Citation :- The Differential Expressions of PPARγ and CAST mRNA in Muscle Tissues of Jinhua and Landrace Pigs.Indian Journal of Animal Research.2021.(55):536-541
Weixin Zhao, Liping Guo, Zhiguo Miao, Jinzhou Zhang, Shan Wang miaozhiguo1998@126.com
Address : College of Animal Science and Veterinary Medicine, Henan institute of Science and Technology, Xinxiang, Henan, 453003, P. R. China. 
Submitted Date : 6-04-2020
Accepted Date : 16-06-2020

Abstract

Background: Fat deposition affected meat quality and the suitable amount of intramuscular fat (IMF) can significantly improve the taste of meat, palatability, juicy, tenderness and flavor, thereby enhancing the meat quality in animals. Although, calpastatin (CAST) and peroxisome proliferator-activated receptor-ã (PPARã) could regulate meat quality by altering meat tenderness or IMF content in animals, reports concerning the relationship between CAST, PPARã expression and pork quality is unclear.
Methods: A total of twenty 28-day-old purebred weaned Jinhua and Landrace piglets (10 piglets per breed) were divided into two groups according to breed for the feeding trials lasting for 120 days. At the 148 days of age, 3 pigs of each breed were selected to slaughter. Total RNA was extracted from longissimus dorsi and biceps femoris muscle to investigate the differential expression of PPARã and CAST in muscle tissues of different breed pigs by RT-PCR methods, as well as their relationship with IMF and carcass lean content.
Result: These results suggested that PPARã is an important effector for regulating fat deposition in pigs, which was correlated with IMF and carcass lean content of pigs. This data would provide a scientific basis for the regulation of pork quality.

Keywords

Carcass lean CAST Intramuscular fat Pig PPARγ
 

References

  1. AOAC, (1990). ‘Official methods of analysis of AOAC international.’ 15th edn. (Association of the Official Analytical Chemists (AOAC) International: Gaithersburg, MD).
  2. Bauer, A., Scheier, R., Eberle, T., Schmidt, H. (2016). Assessment of tenderness of aged bovine gluteus medius muscles using Raman spectroscopy. Meat Science. 115: 27-33. 
  3. Calvo, J.H., Iguácel, L.P., Kirinus, J.K., Serrano, M., Ripoll, G., Casasús, I., Joy, M., Pérez-Velasco, L., Sarto, P., Albertí, P., Blanco, M. (2014). A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness. Meat Science. 96: 775-782.
  4. Chang, Y.C. and Cho, H.J. (2012). Ascofuranone stimulates expression of adiponectin and peroxisome proliferator activated receptor through the modulation of mitogen activated protein kinase family members in 3T3-L1, murine pre- adipocyte cell line. Biochemical and biophysical research communications. 422: 423-428.
  5. Chen, G.S., Chen, S., Sui, Y.N. (2016). Effect of slaughter weight on production and meat quality of Juema pig. Indian Journal of Animal Research. 50:588-594.
  6. Chung, H.Y., Davis, M.E., Hines, H.C. (2001). Genetic variants detected by PCR–RFLP in intron 6 of the bovine calpastatin gene. Animal Genetics. 32:53.
  7. Colle, M.J., Nasados, J.A., Rogers, J.M., Kerby, D.M., Colle, M.M., Van Buren, J.B., Richard, R.P., Murdoch, G.K., Williams, C.J., Doumit, M.E. (2018). Strategies to improve beef tenderness by activating calpain-2 earlier postmortem. Meat Science. 135:36-41.
  8. Cui, J.X., Chen, W., Liu, J., Xu, T., Zeng, Y.Q. (2016). Study on quantitative expression of PPARã and ADRP in muscle and its association with intramuscular fat deposition of pig. Springerplus. 5: 1501.
  9. Djurkin Kušec, I., Kušec, G., Vukoviæ, R., Has-Schön, E., Kralik, G. (2016). Differences in carcass traits, meat quality and chemical composition between the pigs of different CAST genotype. Animal Production Science. 56: 1745-1751.
  10. Freitag, C.M. and Miller, R.J. (2014). Peroxisome proliferator-activated receptor agonists modulate neuropathic pain: a link to chemokines? Front Cell Neurosci. 8: 238.
  11. Gandolfi, G., Pomponio, L., Ertbjerg, P., Karlsson, A.H., Nanni Costa, L., Lametsch, R., Russo, V., Davoli, R. (2011). Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality. Meat Science. 88: 694-700.
  12. Guo, J., Shan, T., Wu, T., Zhu, L.N., Ren, Y., An, S., Wang, Y. (2011). Comparisons of different muscle metabolic enzymes and muscle fiber types in Jinhua and Landrace pigs. Journal of Animal Science. 89: 185-191.
  13. He, K., Wang, Q., Wang, Z., Pan, Y., (2013). Association study between gene polymorphisms in PPAR signaling pathway and porcine meat quality traits. Mammalian Genome. 24: 322-331.
  14. Houbak, M.B., Ertbjerg, P., Therkildsen, M. (2008). In vitro study to evaluate the degradation of bovine muscle proteins post- mortem by proteasome and ì-calpain. Meat Science. 79: 77-85.
  15. Huang, Y., Das, A.K., Yang, Q.Y., Zhu, M.J., Du, M. (2012). Zfp423 promotes adipogenic differentiation of bovine stromal vascular cells. PLoS One. 7: e47496.
  16. Huff-Lonergan, E. and Lonergan, S.M. (2005). Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Science. 71: 194-204.
  17. Hunt, M.R., Garmyn, A.J., O’Quinn, T.G., Corbin, C.H., Legako, J.F., Rathmann, R.J., Brooks, J.C., Miller, M.F. (2014). Consumer assessment of beef palatability from four beef muscles from USDA Choice and Select graded carcasses. Meat Science. 98:1-8.
  18. Insausti, K., Goñi, V., Petri, E., Gorraiz, C., Beriain, M.J. (2005). Effect of weight at slaughter on the volatile compounds of cooked beef from Spanish cattle breeds. Meat Science. 70: 83-90.
  19. Kemp, C.M., Sensky, P.L., Bardsley, R.G., Buttery, P.J., Parr, T. (2010). Tenderness-an enzymatic view. Meat Science. 84: 248-256.
  20. Kersten, S., Desvergne, B., Wahli, W. (2000). Roles of PPARs in health and disease. Nature. 405: 421-424.
  21. Koæwin-Podsiad³a, M., Kury³, J., Krz)cio, E., Zybert, A., Przybylski, W. (2003). The interaction between calpastatin and RYR1 genes for some pork quality traits. Meat Science. 65: 731-735.
  22. Lalitha, S. (2000). Primer premier 5. Biotech Software and Internet Report. 1: 270-272.
  23. Leal-Gutiérrez, J.D., Elzo, M.A., Johnson, D.D., Scheffler, T.L., Scheffler, J.M., Mateescu, R.G. (2018). Association of ì-Calpain and Calpastatin Polymorphisms with Meat Tenderness in a Brahman–Angus Population. Front Genet. 9: 56.
  24. Lee, K.W., Hwang, Y.H., Joo, S.T. (2017). Meat Tenderness Characteristics of Ten Major Muscles from Hanwoo Steers according to Quality Grades of Carcasses. Korean Journal for Food Science of Animal Resources. 37: 593-598.
  25. Li, Y.X., Jin, H.G., Yan, C.G., Seo, K.S., Zhang, L.C., Ren, C.Y., Jin, X. (2013). Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Molecular Biology Reports. 40: 1875-1881.
  26. Ma, J.J., Chai, J., Shang, Y.Y., Li, Y.J., Chen, R., Jia, J., Jiang, S., Peng, J. (2015). Swine PPAR-ã2 expression upregulated in skeletal muscle of transgenic mice via the swine Myozenin-    1 gene promoter. Transgenic Research. 24: 409-420.
  27. Magnabosco, C.U., Lopes, F.B., Fragoso, R.C., Eifert, E.C., Valente, B.D., Rosa, G.J., Sainz, R.D. (2016). Accuracy of genomic breeding values for meat tenderness in Polled Nellore cattle. Journal of Animal Science. 94: 2752-2760.
  28. Miao, Z.G., Wang, L.J., Xu, Z.R., Huang, J.F., Wang, Y.R. (2009). Developmental changes of carcass composition, meat quality and organs in the Jinhua pig and Landrace. Animal. 3:468-473.
  29. Morales, J., Baucells, M.D., Pérez, J.F., Mourot, J., Gasa, J. (2003). Body fat content, composition and distribution in Landrace and Iberian fnishing pigs given ad libitum maize- and acorn-sorghum-maize-based diets. Animal Science. 77: 215-224.
  30. NRC. (1998). ‘Nutrient requirements of swine.’ 10th edn. (National Research Council (NRC): National Academy Press, Washington, DC, USA).
  31. O’Quinn, T.G., Brooks, J.C., Polkinghorne, R.J., Garmyn, A.J., Johnson, B.J., Starkey, J.D., Rathmann, R.J. (2012). Consumer assessment of beef strip loin steaks of varying fat levels. Journal of Animal Science. 90: 626-634.
  32. Ouali, A., Herrera-Mendez, C.H., Coulis, G., Becila, S., Boudjellal, A., Aubry, L., Sentandreu, M.A. (2006). Revisiting the conversion of muscle into meat and the underlying mechanisms. Meat Science. 74: 44-58.
  33. Renaudeau, D., Mourot, I. (2007). A comparison of carcass and meat quality characteristics of Creole and Large White pigs slaughtered at 90 kg BW. Meat Science. 76: 165-171.
  34. Ropka-Molik, K., Bereta, A., Tyra, M., Ró¿ycki, M., Piórkowska, K., Szyndler-Nêdza, M., Szmato³a, T. (2014). Association of calpastatin gene polymorphisms and meat quality traits in pig. Meat Science. 97: 143-150.
  35. Velotto, S., Vitale, C., Varricchio, E., Crasto, A. (2014). A New Perspective: An Italian Autochthonous Pig and Its Muscle and Fat Tissue Characteristics. Indian Journal of Animal Research. 48:143-149.
  36. Wang, H., Xiong, K., Sun, W., Fu, Y., Jiang, Z., Yu, D., Liu, H., Chen, J. (2013). Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of Erhualian pigs. Animal Genetics. 44: 458-462.
  37. Wang, X., Zhang, Y.Q., Zhang, X.Z., Jin, G., Wang, D.C., Li, B., Xu, F., Huang, L.S., Liu, W.Z. (2017). Transcriptome analysis of castrated Bovine reveals the characters of protein Accumulation. Indian Journal of Animal Research. 51: 1043-1050.
  38. Wanger, J.R., Schinckel, A.P., Chen, W., Forrest, J.C., Coe, B.L. (1999). Analysis of body composition changes of swine during growth and development. Journal Animal Science. 77: 1442-1466.
  39. White, B.R., Lan, Y.H., McKeith, F.K., Novakofski, J., Wheeler, M.B., McLaren, D.G. (1995). Growth and body composition of Meishan and Yorkshire barrows and gilts. Journal Animal Science. 73: 738-749.
  40. Wu, J., Liu, Y., Xu, N. (2007). Histological characteristics of longissimus dorsi muscle and their correlation with restriction fragment polymorphisms of calpastatin gene in F2 Jinghua × Piétrain crossbred pigs. Animal. 1: 1237-1242.
  41. Xiao, Y., Kong, F., Xiang, Y., Zhou, W., Wang, J., Yang, H., Zhang, G., Zhao, J. (2018). Comparative biogeography of the gut microbiome between Jinhua and Landrace pigs. Scientific Reports. 8: 5985.
  42. Zeng, Z., Yu, B., Mao, X., Chen, D. (2012). Effects of dietary digestible energy concentration on growth, meat quality, and PPARã gene expression in muscle and adipose tissues of Rongchang piglets. Meat Science. 90: 66-70.
  43. Zhang, H. and Guan, W. (2019). The response of gene expression associated with intramuscular fat deposition in the longissimus dorsi muscle of Simmental × Yellow breed cattle to different energy levels of diets. Animal Science Journal. 90: 493-503.

Global Footprints