Single Nucleotide Polymorphism of CDC37, AHSA1 and STIP1 Gene in Three Cattle Breeds using SNaPshot Technology

DOI: 10.18805/IJAR.B-1266    | Article Id: B-1266 | Page : 378-383
Citation :- Single Nucleotide Polymorphism of CDC37, AHSA1 and STIP1 Gene in Three Cattle Breeds using SNaPshot Technology.Indian Journal Of Animal Research.2021.(55):378-383
Xiao Wang, Guang-Xin E, Ri-Su Na, Cheng-Li Liu, Ze-Hui Guo, Shu-Zhu Cheng, Bai-Gao Yang, Yong-Fu Huang H67738337@swu.edu.cn
Address : College of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Submitted Date : 20-03-2020
Accepted Date : 5-07-2020

Abstract

Background: Heat stress in domestic animals has become a major limit factor for livestock production in tropical and subtropical regions. These traits that adapt to torrid environment are important in livestock breeding.
Methods: In this study, we identified and genotyped six Single nucleotide polymorphism for the CDC37, AHSA1 and STIP1 gene in the Droughtmaster, Angus and Simmental cattle breeds using the SNaPshot Multiplex system. 
Result: Result of pair-wise differences (FST) revealed three SNP locus (CDC37-6 (A16154247/G), CDC37-7 (C16157867/G), AHSA1-10 (G89722567/A) were significantly different in the Droughtmaster population than Angus and Simmental cattle breeds. Moreover, it was found that CDC37-7 (C16157867/G) locus deviated from Hardy-Weinberg equilibrium, suggesting that the CDC37-7 (C16157867/G) genes have been influenced by the selective breeding of the Droughtmaster breed. Therefore, CDC37-7 (C16157867/G) locus could be used as a marker-assisted candidate gene locus of heat tolerance. This study provides valuable information, concerning marker-assisted selection in the breeding of heat stress resistant cattle. 

Keywords

Cattle Heat tolerance traits SNaPshot

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