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Determination of Contagious Agalactia in Sheep and Goats and Investigation of Antibiotic Susceptibility of Mycoplasma spp. Positive Isolates

DOI: 10.18805/ijar.B-1253    | Article Id: B-1253 | Page : 764-768
Citation :- Determination of Contagious Agalactia in Sheep and Goats and Investigation of Antibiotic Susceptibility of Mycoplasma spp. Positive Isolates.Indian Journal of Animal Research.2022.(56):764-768
Nurgül Birben nurbirben23@hotmail.com
Address : Department of Microbiology, Veterinary Control Institute, Zübeyde Hanım Caddesi 160-23200, Elazığ, Turkey.
Submitted Date : 10-02-2020
Accepted Date : 29-04-2020

Abstract

Antibiotic resistance is one of the most important issues encountered globally in managing infectious diseases and is a potential problem in the treatment of Mycoplasma infections. The aims of this study were to: determine carrier rates in sick and healthy herds of sheep and goats; determine the presence in herds of carrier animals that were clinically asymptomatic for contagious agalactia and use antibiogram tests to investigate the susceptibility to antibiotics of Mycoplasma spp. positive isolates derived from sheep and goat ear swabs. The presence of contagious agalactia was diagnosed by analyzing ear swabs (n = 300, bacterial method, Mycoplasma spp.) and blood serum samples (n = 300, serological method [ELISA], Mycoplasma agalactiae) taken from sheep and goat herds located in Elazýð and Malatya provinces in eastern Turkey. The ELISA tests revealed seropositivity in 10 (3.33%) of 300 samples. In order to determine the most effective antibiotic for disease treatment, antibiogram testing was performed on 87 (29%) positive isolates that had been isolated from swab cultures. We determined tulathromycin (MIC 16 µg/mL) and tiamulin (MIC 16 µg/mL) to be the most effective antibiotics, whereas disease agents were resistant to trimethoprim/sulfamethoxazole and neomycin.

Keywords

Antibiotic resistance Antibiogram Bacteriological method Contagious agalactia ELISA Mycoplasma

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