Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 55 issue 6 (june 2021) : 674-678

Effect of Cellulase Genes Recombinant L. reuteri on Digestibility of Diet Crude Fiber and Adhesion in Gut of Chicken

Wang Li, Jingbo Zhang, Peng Cheng, Longmei Zhao
1College of Animal Science and Technology, Henan University of Science and Technology, Luo Yang, China.
Cite article:- Li Wang, Zhang Jingbo, Cheng Peng, Zhao Longmei (2020). Effect of Cellulase Genes Recombinant L. reuteri on Digestibility of Diet Crude Fiber and Adhesion in Gut of Chicken. Indian Journal of Animal Research. 55(6): 674-678. doi: 10.18805/ijar.B-1241.

ABSTRACT

Background: The aim of this test was study the gut adhesion ability of recombinant Lactobacilli and their improvement of the digestibility of crude fiber in chick diet. 
Methods: The adhesion ability was observed through in vitro and in vivo tests. Intestinal epithelial cells and recombinant Lactobacillus were co-cultured for observation of adhesion. One hundred one-day-old chicks were randomly divided into four groups; C15, C73, C1573 and control. Each chick in C15, C73, C1573 and control were orally gavaged with 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel15, 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel73 and 200 ìL of broth containing equal XNY-Cel15 and XNY-Cel73, 200 ìL of MRS broth, once at the beginning of the trial respectively. 
Result: Recombinant L. reuteri adhered to chick intestinal epithelial cells which co-cultured together in vitro. After feeding for 30 days, the reporter genes of recombinant Lactobacillus was detected in the crop, small intestine and cecum of trial chicks, with their sequences corresponding to those of the insert genes. The digestibility of crude fiber in experimental groups was higher than that in control group and the digestibility of C15 was significantly higher than other groups (P<0.01). It showed that the recombinant Lactobacillus can survive in the digestive tract of chicken for more than 30 days and helped to decompose the crude fiber in the digestive tract.

REFERENCES

  1. Chen K.-L., Chang H.-J., Yang C.-K., You S.-H., Jenq H.-D. and Yu B. (2007). Effect of dietary inclusion of dehydrated food waste products on Taiwan native chicken (Taishi No. 13). Asian-Australasian Journal of Animal Sciences. 20: 754-760.
  2. Conlon M.A. and Kita K. (2002). Muscle protein synthesis rate is altered in response to a single injection of insulin-like growth factor-I in seven-day-old Leghorn chicks. Poultry Science. 81: 1543.
  3. Dalloul R.A., Lillehoj H.S., Tamim N.M., Shellem T.A. and Doerr J. A. (2005). Induction of local protective immunity to Eimeria acervulina by a Lactobacillus-based probiotic. Comparative Immunology, Microbiology and Infectious Diseases. 28: 351-361.
  4. Edelman S., Leskela S., Ron E., Apajalahti J. and Korhonen T.K. (2003). In vitro adhesion of an avian pathogenic Escherichia coli O78 strain to surfaces of the chicken intestinal tract and to ileal mucus. Veterinary Microbiology. 91: 41-56.
  5. Fahey A.G., Marchantforde R.M. and Cheng H.W. (2007). Relationship Between Body Weight and Beak Characteristics in One-Day-Old White Leghorn Chicks: Its Implications for Beak Trimming. Poultry Science. 86: 1312-5.
  6. Fortineau N., Trieu-Cuot P., Gaillot O., Pellegrini E., Berche P. and Gaillard J.-L. (2000). Optimization of green fluorescent protein expression vectors for in vitro and in vivo detection of Listeria monocytogenes. Research in Microbiology. 151: 353-360.
  7. Ginindza M. M., Ng’Ambi J. W. and Norris D. (2017). Effect of dietary crude fibre level on intake, digestibility and productivity of slow-growing indigenous Venda chickens aged one to 91 days. Indian Journal of Animal Research. 51: 1073-1079.
  8. Lähteinen T., Malinen E., Koort J.M.K., Mertaniemihannus U., Hankimo T., Karikoski N., Pakkanen S., Laine H., Sillanpää H. and Söderholm H. (2010). Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe. 16: 293.
  9. Lam, E.K.Y., Yu, L., Wong, H.P.S., Wu, W.K.K., Shin, V.Y., Tai, E. K.K., So, W.H.L., Woo, P.C.Y. and Cho, C.H. (2007). Probiotic Lactobacillus rhamnosus GG enhances gastric ulcer healing in rats. European Journal of Pharmacology. 565: 171-179.
  10. Li, W., Huan, X., Zhou, Y., Ma, Q. and Chen, Y. (2009). Simultaneous cloning and expression of two cellulase genes from Bacillus subtilis newly isolated from Golden Takin (Budorcas taxicolor Bedfordi). Biochemical and Biophysical Research Communications. 383: 397-400.
  11. Li, W., Yang, M.-M., Zhang, G.-Q., He, W.-L., Li, Y.-X. and Chen, Y.-L. (2012). Electrotransformation and Expression of Cellulase Genes in Wild-Type Lactobacillus reuteri Journal of Molecular Microbiology and Biotechnology 22: 228-234.
  12. Lin, X.Y. (2006). The research of adhesion of Lactobacillus in chicken gut, Vol. Thesis, Shandong agriculture university. Taian.
  13. Liu, J.R., Lai, S.F. and Yu, B. (2007). Evaluation of an intestinal Lactobacillus reuteri strain expressing rumen fungal xylanase as a probiotic for broiler chickens fed on a wheat-based diet. Br Poult Sci. 48: 507-14.
  14. Liu, M. and Yu, H. (2012). Co-production of a whole cellulase system in Escherichia coli. Biochemical Engineering Journal. 69: 204-210.
  15. Liu, Q., Zhang, W.M., Zhang, Z.J., Zhang, Y.J., Zhang, Y.W., Chen, L. and Zhuang, S. (2016). Effect of fiber source and enzyme addition on the apparent digestibility of nutrients and physicochemical properties of digesta in cannulated growing pigs. Animal Feed Science and Technology. 216: 262-272.
  16. Lv, L.-X., Yan, R., Shi, H.-Y., Shi, D., Fang, D.-Q., Jiang, H.-Y., Wu, W.-R., Guo, F.-F., Jiang, X.-W., Gu, S.-L., Chen, Y.-B., Yao, J. and Li, L.-J. (2017). Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01. Journal of Proteomics. 150: 216-229.
  17. Radhika, G., Subriya, S., Manjari, K.D., Parthiban, M., Pazhanivel, N. and Vijayarani, K. (2020). Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken. Indian Journal of Animal Research. 54: 216-221.
  18. Rafique K., Rahman A. and Mahmood M. (2020). Effect of dietary supplementation of different levels of saccharomyces cerevisiae on growth performance and hematology in broiler. Indian Journal of Animal Research. 54: 59-64.
  19. Ravindran, V., Tilman, Z.V., Morel, P.C.H., Ravindran, G. and Coles, G.D. (2007). Influence of â-glucanase supplementation on the metabolisable energy and ileal nutrient digestibility of normal starch and waxy barleys for broiler chickens. Animal Feed Science and Technology. 134: 45-55.
  20. Soest, P.J.V., Robertson, J.B. and Lewis, B.A. (1991). Methods for Dietary Fiber, Neutral Detergent Fiber and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science. 74: 3583-3597.
  21. Song, Y., Lee, Y.G., Choi, I.S., Lee, K.H., Cho, E.J. and Bae, H.-J. (2013). Heterologous expression of endo-1,4-â-xylanase A from Schizophyllum commune in Pichia pastoris and functional characterization of the recombinant enzyme. Enzyme and Microbial Technology. 52: 170-176.
  22. Tierney, J., Gowing, H., Van, Sinderen, D., Flynn, S., Stanley, L., McHardy, N., Hallahan S. and Mulcahy G. (2004). In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species. Veterinary Parasitology. 122: 171-182.
  23. Yu, B., Liu, J.R., Chiou, M.Y., Hsu, Y.R. and Chiou, P.W.S. (2007). The Effects of Probiotic Lactobacillus reuteri Pg4 Strain on Intestinal Characteristics and Performance in Broilers Asian-Aust. J. Anim. Sci. 20: 1243-1251.
  24. Zhang, Y., Xiang, X., Lu, Q., Zhang, L., Ma, F. and Wang, L. (2016). Adhesions of extracellular surface-layer associated proteins in Lactobacillus M5-L and Q8-L. Journal of Dairy Science. 99: 1011-1018. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)