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Clinico-Pathological and Hemato-Biochemical Assessment of Field Originated Chicken Anemia Virus in Experimentally Challenged Broiler Chicken

DOI: 10.18805/ijar.B-1229    | Article Id: B-1229 | Page : 936-940
Citation :- Clinico-Pathological and Hemato-Biochemical Assessment of Field Originated Chicken Anemia Virus in Experimentally Challenged Broiler Chicken.Indian Journal of Animal Research.2021.(55):936-940
Syed Maaz Nadeem, Muti ur Rehman Khan, Asim Aslam, Ali Ahmad Sheikh, Arfan Ahmad, Asma Abdul Latif, Ahsan Anjum maaznadeem@hotmail.com
Address : Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Submitted Date : 23-11-2019
Accepted Date : 2-05-2020

Abstract

One hundred broiler chicks were divided into two groups A and B. The chicks in group A were control group and group B was challenged with field originated chicken infectious anemia virus (CIAV) at 7th day of age. The samples were collected on 7th, 14th, 21st, 28th and 35th day of post infection for hematological, biochemical, immunohistochemical and histopathological examination. The hematological analysis showed significantly (P<0.05) low level of hemoglobin, decreased packed cell volume, leukocytopenia and thrombocytopenia in infected group as compared to control group. In immunohistochemical assay, CIAV was detected in portal zone of liver, epicardium and myocardium, lymphoid follicles of bursa of Fabricius, thymic lobules and mainly white pulp of spleen. Histopathological changes were observed in both lymphoid and non-lymphoid organs. Concerning the serum biochemical profile, hypoproteinemia, hypoalbuminemia, increased concentration of liver enzymes, creatinine and urea were observed in infected birds. Our results showed that field originated stra in is pathogenic for broilers which induce significant changes in hematology, serum biochemical profile and histopathology of lymphoid and non-lymphoid organs.

Keywords

​​CAV Hematology Histopathology Immunohistochemistry PCR

References

  1. Al-Hassani A.S. (2011). Effect of dietary supplementation with different levels of arginine on some blood traits of laying hens. Intl. J. Poult. Sci. 10 (9): 705-709.
  2. Goodwin M.A., Davis J.F. and Brown J. (1992a). Packed cell volume reference intervals to aid in the diagnosis of anemia and polycythemia in young broiler chickens. Avian Dis. 36 (2): 440-443.
  3. Goodwin M., Brown J., Davis J., Girshick T., Miller S., Nordgren R. and Rodenberg J. (1992b). Comparisons of packed cell volumes (PCVs) from so-called chicken anemia agent (CAA; a virus)-free broilers to PCVs from CAA-free specific-pathogen-free leghorns. Avian Dis. 36 (4): 1063-1066.
  4. Haridy M., Goryo M., Sasaki J. and Okada K. (2009). Pathological and immunohistochemical study of chickens with co-infection of Marek’s disease virus and chicken anaemia virus. Avian Pathol. 38 (6): 469-483.
  5. Hermann J., Koski D., Taylor S. and Gatewood D. (2012). Evaluation of the analytical sensitivity of a polymerase chain reaction assay for the detection of chicken infectious anemia virus in avian vaccines. Biologicals. 40 (4): 266-269.
  6. Karimi I., Mahzounieh M., Bahadoran S. and Azad F. (2010). Chicken anemia virus infection in broiler chickens in Shahrekord, Iran: serological, hematological and histopathological findings. Comp. Clin. Path. 19 (1): 63-67.
  7. Khanam F., Iqbal M.N., Ashraf A., Alam S., Muhammad A., Xiao S., Toor S. and Mumtaz H. (2016). Evaluation of Changes in Liver Enzymes in Broiler Chicks (Gallous domesticus). PSM Vet. Res. 1 (1): 26-31.
  8. Krishan G., Shukla S.K., Bhatt P., Kumar R., Tiwari R., Malik Y.S. and Dhama K. (2015). Immunomodulatory and protective effects of a polyherbal formulation (immon) against infectious anemia virus infection in broiler. Int. J. Pharmacol. 11 (5): 470-476.
  9. Kuscu B. and Gürel A. (2008). Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease. J. Vet. Sci. 9 (1): 15-23.
  10. Li Y., Fang L., Cui S., Fu J., Li X., Zhang H., Cui Z., Chang S., Shi W. and Zhao P. (2017). Genomic Characterization of Recent Chicken Anemia Virus Isolates in China. Front. Microbiol. 8 401.
  11. McNulty M. (1991). Chicken anaemia agent: a review. Avian Pathol. 20(2): 187-203.
  12. Najm-ul-Islam M.K.S., Khan M.Z., Butt S.L., Khan A., Javed I. and ShahidRafique F.S.A.a. (2013). Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan. Pak. Vet. J. 33 (3): 378-381.
  13. Oluwayelu D.O. (2010). Diagnosis and epidemiology of chicken infectious anemia in Africa. Afric. J. Biotec. 9 (14): 2043-2049.
  14. Schat K.A., da Silva Martins N.R., O’Connell P.H. and Piepenbrink M.S. (2011). Immune complex vaccines for chicken infectious anemia virus. Avian Dis. 55 (1): 90-96.
  15. Spackman E., Cloud S., Pope C. and Rosenberger J. (2002). Comparison of a putative second serotype of chicken infectious anemia virus with a prototypical isolate I. Pathogenesis. Avian Dis. 46 (4): 945-955.
  16. Vaziry A., Silim A., Bleau C., Frenette D. and Lamontagne L. (2011). Chicken infectious anaemia vaccinal strain persists in the spleen and thymus of young chicks and induces thymic lymphoid cell disorders. Avian Pathol. 40 (4): 377-385.

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