Research of Mating System in Tupaia belangeri by Microsatellite DNA Markers

DOI: 10.18805/ijar.B-1201    | Article Id: B-1201 | Page : 1098-1102
Citation :- Research of Mating System in Tupaia belangeri by Microsatellite DNA Markers.Indian Journal Of Animal Research.2020.(54):1098-1102
Dong-min Hou, Ting Jia, Li-xin Chen, Zheng-kun Wang, Wan-long Zhu houl92@163.com
Address : Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Science of Yunnan Normal University, Kunming 650500, China. 
Submitted Date : 18-09-2019
Accepted Date : 18-03-2020

Abstract

Mating system of a population refers to the general behavioral strategies which employed in obtaining mates. In order to investigate the genetic parentage of 151 individuals in Tupaia belangeri from Haikou Forest Farm, 8 microsatellite loci with higher polymorphic characters were used for genetic relationship analysis. The results showed that the number of alleles per polymorphic locus in 151 individuals ranged from 2 to 9. The average observed and expected heterozygosities per locus ranged from 0.2476 to 0.8367 and from 0.2789 to 0.7773, respectively. While the polymorphic information content ranged from 0.2469 to 0.7773. Except the locus (TBC1) deviated from Hardy–Weinberg equilibrium, the others 7 microsatellite loci were employed to research the mating system in T. belangeri, which found that non-strictly monogamous in T. belangeri, but exhibited evidence of multiple paternity. All of the results suggested that characteristics of 8 microsatellite loci provide an excellent tool for the research of mating systems and fine-scaled population structuring of tree shrew, which was the first molecular evidence for mating system in T. belangeri.

Keywords

Extra-pair paternity Mating system Microsatellite DNA Tupaia belangeri

References

  1. Capy, P. and Brookfield, J.F.Y. (1991) Estimation of relatedness in natural populations using highly polymorphic genetic markers. Genetics Selection Evolution. 23: 391-406.
  2. Carter, C., Devries, A. and Getz, L. (1995) Physiological substrates of mammalian monogamy: the prairie vole model. Neuro- -science and Biobehavioral Reviews. 19: 303–314.
  3. Cheverud, J.M., Dow, M.M. and Leutenegger, W. (1985). The quantitative assessment of phylogenetic constraints in comparative analyses: sexual dimorphism in body weight among primates. Evolution. 39: 1335–1351.
  4. Collins, P.M. and Tsang, W.N. (1987) Growth and reproductive development in the male tree shrew (Tupaia belangeri) from birth to sexual maturity. Biology of Reproduction. 37: 261–267.
  5. Collins, P.M., Tsang, W.N. and Lofts, B. (1982). Anatomy and function of the reproductive tract in the captive male tree shrew (Tupaia belangeri). Biology of Reproduction. 1: 169-182.
  6. Dixson, A. (2005) Mating systems and strategies. American Journal of Human Biology. 17: 390-391.
  7. Emlen, S. and Oring, L. (1977). Ecology, sexual selection and the evolution of mating systems. Science. 197: 215-223.
  8. Jing, X.L., Wang, X.X. and Wang, W.G. (2011). Isolation of micro- -satellite markers in tree shrews (Tupaia belangeri chinensis). Acta Laboratorium Animalis Scientia Sinica. 19: 312–315.
  9. Kawamichi, T. and Kawamichi, M. (1979). Spatial organization and territory of three shrews (Tupaia glis). Animal Behaviour. 27: 381–393.
  10. Kawamichi, T. and Kawamichi, M. (1982). Social system and independence of offspring in tree shrews. Primates. 23: 189–205.
  11. Kleiman, D.G. and Malcolm, J.R. (1981). The evolution of male parental investment in mammals. The Quarterly Review of Biology. 52: 39–68.
  12. Kleiman, D.G. (1977). Monogamy in mammals. Quarterly Review of Biology. 52: 39–45.
  13. Liu, X.H. and Yao, Y.G. (2013). Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis). Zoological Research. 34: E62–E68. 
  14. Martin, R. (1966). Tree shrews: unique reproductive mechanism of systematic importance. Science. 152: 1402–1404.
  15. Martin, R. (1968). Reproduction and ontogeny in treeshrews (Tupaia belangeri), with reference to their general behaviour and taxonomic relationships. Zeitschrift fuer Tierpsychologie. 25: 409–532.
  16. Miño, C.I., Russello, M.A., Mussi Gonçalves, P.F. and Del Lama, S.N. (2011). Reconstructing genetic mating systems in the absence of parental information in colonially breeding waterbirds. BMC Evolutionary Biology. 11: 196.
  17. Reynolds, J.D. (1996) Animal breeding systems. Trends in Ecology and Evolution. 11: 68–72.
  18. Schwaier, A. (1973) Breeding tupaias (Tupaia belangeri) in captivity. Zeitschrift Fur Versuchstierkunde. 15: 251–255. 
  19. Schwaier, A. (1974). Cage for the successful maintenance of tree shrews (Tupaia belangeri) in captivity. Zeitschrift Fur Versuchstierkunde. 16: 337-340.
  20. Selolo, T.C., Mashiloane, M.L., Norris, D., Ng’ambi, J.W. and Brown, D. (2015). Morphological differentiation of indigenous goats in different agro-ecological zones of Vhembe district, Limpopo province, South Africa. Indian Journal of Animal Research. 49: 527–531.
  21. Sinha, B., Mandal, K.G., Kumari, R, Kumari, A. and Gonge, D.S. (2018). Genetic impact on external and internal egg quality traits of Vanaraja and Gramapriya birds and their crosses in Bihar. Indian Journal of Animal Research. 52: 953–958.
  22. Slate, J., Marshall, T. and Pemberton, J. (2000). A retrospective assessment of the accuracy of the paternity inference program cervus. Molecular Ecology. 9: 801–808.
  23. Sorenson, M.W. and Conaway, C.H. (1966). Observations on the social behavior of tree shrews in captivity. Folia Primato- -logica. 4: 124-145.
  24. Wang, Z.K., Sun, R.Y., Li, Q.F. and Fang, J.M. (1994). Characteristics of the resting metabolic rate of tree shrew, Tupaia belangeri. Journal of Beijing Normal University (Natural Science). 30: 408-414.
  25. Zhu, W.L., Ren, X.Y., Hou, D.M., Wang, W.Q. and Wang, Z.K. (2019) Analysis of volatile chemical substances in urine of the kinship and non-kinship Tupaia belangeri (Mammalia: Scandentia: Tupaiidae). Indian Journal of Animal Research. 53: 311-320.

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