Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 10 (october 2020) : 1235-1240

Overexpression of hemA and hemL in Bacillus subtilis Promotes Overexpression of 5-aminolevulinic Acid 

Jinni Liu, Zhaowei Ye, Haigang Wu, Jicheng Liu, Yuesheng Gong
1Laboratory of Veterinary Pharmacology and Toxicology, College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang, 464 000, PR China.
Cite article:- Liu Jinni, Ye Zhaowei, Wu Haigang, Liu Jicheng, Gong Yuesheng (2020). Overexpression of hemA and hemL in Bacillus subtilis Promotes Overexpression of 5-aminolevulinic Acid. Indian Journal of Animal Research. 54(10): 1235-1240. doi: 10.18805/ijar.B-1193.

ABSTRACT

Bacillus subtilis strains were constructed that overexpressed the glutamyl t-RNA reductase (HemA) and glutamyl t-RNA synthase (HemL). The target proteins accounted for 11 and 13% of the total soluble protein in maltose induced extracts, respectively. The culture media from these strains was red with wavelength maxima near 400 nm indicating elevated levels of porphyrins. The 5-aminolevulinic acid content for these strains reached 42.57 and 39.78 mg/L after 24 h of induced growth and 65.23 and 68.45 mg/L, respectively, after 60 h. Therefore, hemA and hemL overexpression enhanced the biosynthesis of 5-aminolevulinic acid and these strains can be used for its purification.

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