29-kDa: A Potential Candidate for Anti-Tick Vaccine Antigen Source as Immunogenic and Stage Reactive Targeting Hard-Bodied Hyalomma Ticks (Ixodidae)

DOI: 10.18805/ijar.B-1191    | Article Id: B-1191 | Page : 71-77
Citation :- 29-kDa: A Potential Candidate for Anti-Tick Vaccine Antigen Source as Immunogenic and Stage Reactive Targeting Hard-Bodied Hyalomma Ticks (Ixodidae).Indian Journal Of Animal Research.2021.(55):71-77
Kashif Kamran, Cristian A. Villagra, Asim Iqbal, Asmatullah Kakar, Constaza Schapheer, Muhammad Kamran Taj, Abid Ali, Saima Siddiqui kashifkamran944@gmail.com
Address : Department of Zoology, University of Balochistan Quetta Pakistan.
Submitted Date : 15-08-2019
Accepted Date : 20-12-2019

Abstract

The objective of present study is to develop a vaccine against Hyalomma hard-bodied tick and to analyze relevant experimental data on immunized indigenous horse breed Morna. Montanide (ISA-50) adjuvant-based vaccine induced significantly (p < 0.02) higher antibody titre through intradermal route with 99.98% vaccine efficacy. Humoral response was determined though indirect ELISA; where peak level of serum antibody was recorded after six weeks interval of post-immunization. Most of hematology and biochemical parameters remained consistent to normal reference values. Our report also indicates a significant percentage decline in the numbers of engorged ticks, eggs mass, eggs number and increased tick rejection. The animal travel history can promote tick burden and is a potential risk factor. Based on biological and hematological parameters, it is possible to conclude that 29-kDa antigen can be used as an effective antigen vaccine candidate to control tick infestation detected through humoral response.

Keywords

Adjuvant Anti-tick vaccine ELISA Risk factor 29-kDa

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