Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 55 issue 1 (january 2021) : 78-83

Phytochemical Screening, In-vitro and In-vivo Anti-diabetic Activity of Nelumbo nucifera Leaves against Alloxan-Induced Diabetic Rabbits

Shahid Maqbool, Najeeb Ullah, Aqal Zaman, Atif Akbar, Saima Saeed, Haq Nawaz, Noreen Samad, Riaz Ullah, Ahmed Bari, Syed Saeed Ali
1Department of Biochemistry, Bahauddin Zakariya University, Multan, Punjab, Pakistan.
Cite article:- Maqbool Shahid, Ullah Najeeb, Zaman Aqal, Akbar Atif, Saeed Saima, Nawaz Haq, Samad Noreen, Ullah Riaz, Bari Ahmed, Ali Saeed Syed (2019). Phytochemical Screening, In-vitro and In-vivo Anti-diabetic Activity of Nelumbo nucifera Leaves against Alloxan-Induced Diabetic Rabbits. Indian Journal of Animal Research. 55(1): 78-83. doi: 10.18805/ijar.B-1181.
A recent survey has demonstrated that traditional healers and herbalists frequently use Nelumbo nucifera plant to treat diabetes. The plant investigated here has been previously reported as natural folk medicine, but until now no scientific investigation of potential anti-diabetic effects have been reported. Plant leaves of Nelumbo nucifera were screened out for quantitative analysis of phytochemicals, in-vitro anti-diabetic activity (Alpha-amylase inhibitory assay) and In-vivo anti-diabetic assay of animals (rabbits). Quantitative analysis indicates that different phytochemicals were present in plant leaves extract/fractions. In-vitro antidiabetic analysis results poses that butanol fraction is significantly more active than other fractions or extract in antihyperglycemic activity with minimum IC50 value of 8.56µg/ml. For In-vivo anti-diabetic assay, animals (rabbits) were examined and animals were divided into 10 groups and except blank group all were treated with standard and plant extracts/fractions doses (25mg and 50mg per Kg body weight) as an aqueous suspension. Their blood sugar level was thoroughly recorded with a glucometer before the administration of sample and standard drug. Alloxan Monohydrate was administrated orally (as an ice-cold water solution) at dose rate 150mg/kg body weight for one week to make animals hyperglycemic. In-vivo analysis indicates that butanol fraction is the most active fraction in antihyperglycemic activity. So Leaves of N. nucifera can be used as antidiabetic remedy. So, it is strongly recommended that N. nucifera leaves should be elucidated further for isolation of active phytochemicals.  
  1. Abdelaaty A. Shahat , Riaz Ullah, et al. (2018). Hepatoprotective Effect of Eriobotrya japonica Leaf Extract and Its Various Fractions against Carbon Tetra Chloride Induced Hepatotoxicity in Rats. Evidence-Based Complementary and Alternative Medicine, 2018, 8 pages.
  2. Abidemi OO. (2013). Phytochemicals and Spectro photometric Determination of Metals in Various Medicinal Plants in Nigeria. International Journal of Engineering Science Invention. 2(5): 51–54.
  3. Ahmed I, Naeem M, Zaheer ASA, Hafiz M. (2010). Investigation of the anti-diabetic and hypocholesterolemic potential of psyllium husk fiber (Plantago psyllium) in diabetic and hypercholesterolemic albino rats. International Journal of Biomedical Science and Engineering. 6: 185–189
  4. Bhardwaj A, Modi KP. (2015). Pharmacognostical, Phytochemical Screening and Standardization Of Nelumbo Nucifera (Gaertn): The Sacred Lotus. International Journal of Pharmacy and Pharmaceutical Sciences. 8(1): 144–149.
  5. Hussain I, Ullah R, Khurram M, Ullah N, Baseer A, Khan FA, Khan N. (2011). Phytochemical analysis of selected medicinal plants. African Journal of Biotechnology. 10(38): 7487–7492.
  6. Jong Hoon Ahn, Seon Beom Kim, Eun Sil Kim, Soonok Kim, Soo-    Hyun Cho, Bang Yeon Hwang and Mi Kyeong Lee (2014). A new flavolignan from Nelumbo nucifera LEAVES Chemistry of Natural Compounds. 50(6): 998-1000.
  7. Jyoti Rani, Manish Kapoor* and Rupinder Kaur (2017). In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don. Agric. Sci. Digest. 37(2): 106-111.
  8. Keshav Raj Paudel and Nisha Panth. (2015). Phytochemical Profile and Biological Activity of Nelumbo nucifera Evidence-    Based Complementary and Alternative Medicine Volume 2015, 16 pages.
  9. Kim T, Kim HJ, Cho SK, Kang WY, Baek H, Jeon HY, Kim D. (2011). Nelumbo nucifera Extracts as whitening and anti-wrinkle cosmetic agent. Korean Journal of Chemical Engineering. 28(2): 424–427.
  10. P.K. Deepa, P.T.A. Usha, A.M.C. Nair, and K.T.Prasannakumari (2008) Assessment Of Cns activity of Nelumbo Nucifera (Red and White type) using Rotarod. Indian J. Anim. Res. 42(4): 273-275.
  11. Rege AAA, Chowdhary AS. (2014). Evaluation of Alpha-Amylase and Alpha-Glucosidase Inhibitory Activities of Shilajit. International Journal of Advanced Research. 2(2): 735–40.
  12. Rodin G, Olmsted MP, Rydall AC, Maharaj SI, Colton PA, Jones JM, Daneman D. (2002). Eating disorders in young women with type 1 diabetes mellitus. Journal of Psychosomatic Research. 53(4): 943–949.
  13. Sharma BR, Rhyu DY. (2014). Anti-diabetic effects of Caulerpa lentillifera: stimulation of Insulin secretion in pancreatic â-cells and enhancement of glucose uptake in adipocytes. Asian Pacific Journal of Tropical Biomedicine. 4(7): 575–580.
  14. Sultan K, Zakir M, Khan H, Khan IU, Ayaz S, Khan I, Khan MA. (2016). Antihyperglycemic effect of Persea duthieion blood glucose levels and body weight in alloxan induced diabetic rabbits. Pakistan Journal of Pharmaceutical Sciences. 29(3): 837–842.
  15. V. O. Ayodele, O. M. Olowe (2019) Morphological traits and Nastism of Mango ginger (Curcuma amada Roxb.), Agricultural Science Digest. Volume 39: 177-183.
  16. Yang M-Y, Chang Y-C, Chan K-C, Lee Y-J, Wang C-J. (2011). Flavonoid-enriched extracts From Nelumbo nucifera leaves inhibits proliferation of breast cancer in vitro and in vivo. European Journal of Integrative Medicine. 3(3): 153–163.

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