Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 8 (august 2020) : 1000-1005

Anti-inflammatory, Antipyretic and Analgesic Activities of Persimmon (Diospyros kaki) Leaves in Animal Model

Abdelaaty A. Shahat, Ali S. Alqahtani, Riaz Ullah, Abdullah Al-Mishari, Wafaa Sabry Ahmed
1Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
Cite article:- Shahat A. Abdelaaty, Alqahtani S. Ali, Ullah Riaz, Al-Mishari Abdullah, Ahmed Sabry Wafaa (2020). Anti-inflammatory, Antipyretic and Analgesic Activities of Persimmon (Diospyros kaki) Leaves in Animal Model. Indian Journal of Animal Research. 54(8): 1000-1005. doi: 10.18805/ijar.B-1170.

ABSTRACT

Diospyros kaki L. (Ebenaceae) fruit and leaves are traditionally used for the treatment of hypertension and angina, as well as for their antithrombotic and anti-inflammatory effects. We investigated the anti-inflammatory, analgesic and antipyretic activities of Diospyros kaki L. (Ebenaceae) in order to scientifically validate its pharmacological importance. In the current study, the anti-inflammatory, analgesic and antipyretic activities of ethyl acetate (Per-1), n-butanol (Per-2) and aqueous (Per-3) fractions of a methanol extract of Persimmon leaves were tested. Wistar rats weighing 190–210 g (for the anti-inflammatory assay) and male albino mice (Swiss) weighing 18–24 g (for the antipyretic and analgesic assays) were utilized as the experimental animals. The rats were separated into eight groups (for anti-inflammatory) and seven group (for antipyretic), each consisting of six rats. Each sample was treated orally and two doses: 100 and 200 mg/kg body weight were used in these experiments. The Per-3 sample at a dose of 200 mg/kg produced the highest inflammation inhibition at 59.28%. The same sample at a dose of 200 mg/kg lowered body temperature from 38°C of hyperthermia mice back to 36.65 ± 0.12°C after 120 min, close to the normal temperature (35.21 ± 0.29°C). In the tail flick model, sample Per-3 showed 67.74% highest nociception inhibition. Similar results were obtained with Acetic acid and hot-plate assay. These results confirm that the n-butanol and aqueous fractions of persimmon are active and may be used for further pharmacological screening.

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