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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 54 issue 9 (september 2020) : 1176-1178,   Doi: 10.18805/ijar.B-1168

Polymorphism Analysis of FMO3 Gene in Two Strains Quails

J
Jun Yan Bai
X
Xiao Ping Jia1
Y
You Bing Yang
G
Guang Lu Li
H
Hong Deng Fan
H
Heng Cao
X
Xue Yan Fu
K
Kun Peng Shi
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. 
Cite article:- Bai Yan Jun, Jia1 Ping Xiao, Yang Bing You, Li Lu Guang, Fan Deng Hong, Cao Heng, Fu Yan Xue, Shi Peng Kun (2019). Polymorphism Analysis of FMO3 Gene in Two Strains Quails. Indian Journal of Animal Research. 54(9): 1176-1178. doi: 10.18805/ijar.B-1168.

ABSTRACT

In this study, polymorphism of FMO3 genes in French giant quail and Savimit quail was tested by PCR technology and hybrid DNA pool sequencing technology. The results show that, one SNPs mutation site (A66G) was detected from the exon 2 of FMO3, the A and G allele frequencies of A66G in French giant quail and Savimit quail were 0.586/0.414 and 0.395/0.605. Two SNPs mutation sites (A139T and C187T) were detected from the exon 4 of FMO3, A and T allele frequencies of A139T in French giant quail and Savimit quail were 0.630/0.370 and 1.000 /0.000, C and T allele frequencies of C187T in French giant quail and Savimit quail were 0.256/0.744 and 0.763/0.237, respectively. Two mutation sites (A39G and A41T) were detected from exon 7, A and G allele frequencies of A39G in French giant quail and Savimit quail were 0.769/0.231 and 0.190 /0.810, A and T allele frequencies of A41T were 0.107/0.893 and 0.000/1.000. One SNPs mutation site (C219T) was detected from exon 9, C and T allele frequencies of C219T in French giant quail and Savimit quail were 0.250/0.750 and 0.250/0.750, respectively. According to clustering analysis, 10 different livestock species were finally clustered into three groups. Quail had close genetic relationship with jungle fowl and they were clustered into one group. The tropical xenopus was clustered into the second group. The rest animals were clustered into the third group. 

REFERENCES

  1. Bai, J.Y., Jia, X.P., Yang, Y.B., Pang, Y.Z. and Wang, Y.Q. (2017b). Study on the polymorphism of fibroblast growth facor receptor (FGFR1) in sheep. Indian J. Anim. Res. 51: 856-859.
  2. Bai, J.Y., Wang, X., Yang, Y.B., Zhang, X.H., Pang, Y.Z. and Li, H.W. (2016e). Study on the polymorphism of POU1F1 gene in sheep. R. Bras. Zootec. 45: 604-607. https://doi.org/ 10.1590/S1806- 92902016001000004.
  3. Bai, J.Y., Zhao, Y.G., Li, G.L., Yang, Y.B., Wang, Y.Q. , Wang ,X. and Yang, S. (2019). Analysis of polymorphism of growth hormone secretagogue receptor in goat. Indian Journal of Animal Research. 53(7): 856-859.
  4. Bai,J.Y., Pang,Y.Z.,Qi,Y.X., Zhang,X.H. and Yun,Y.X.(2017a). Analysis of the polymorphism of expressed sequence tag-Simple sequence repeat in quail. Indian Journal of Animal Research. 51(5): 851-855. DOI:10.18805/ijar.v0i0f.3803
  5. Bai,J.Y., Pang,Y.Z.,Wu,S.J., Yu,M.Q. and Zhang,X.H.(2016a). Analysis of genetic diversity of four quails by microsatellite markers. Indian Journal of Animal Research. 50(1): 1-7. DOI: 10.18805/ ijar.8429
  6. Bai,J.Y., Pang, Y.Z., Zhang, X.H., Yun, Y.X. and Qi, Y.X.(2016b). Microsatellite Analysis of Genetic Diversity in Quail Populations from China. Brazilian Journal of Poultry Science. 18(3): 519-524. http://dx.doi.org/ 10.1590 /1806-9061-2015-0101.
  7. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, X.Y. (2016c). Development and application of Est-Ssr Markers in quails, Brazilian Journal of Poultry Science. 18(Special Issue):027-032. http://dx.doi.org/10.1590/1806-9061-2015-0124.
  8. Bai, J.Y., Pang, Z., Zhang, X.H. and Li, Y.X. (2016d). Study on the Morphological Development of quail Embryos.Brazilian Journal of Poultry Science, 18(Special Issue2):033-036. http://dx.doi.org/10.1590/1806-9061-2015- 0177. 
  9. Li, Y.X., Zhang, X.H., Pang, Y.Z., Qi, Y.X. and Zhao, S.J. (2019). Construction of MC1R and ASIP Eukaryotic expression vector and its regulation of plumage color in Japanese quail (Coturnix japonica). Journal of Poultry Science. 56(2): 84-90.
  10. Mo, F.T. (2014). Cloning and polymorphism of quail FMO3 gene associated with Trimethylamine content in Egg Yolk. Ph.D. Dissertation of China Agricultural University.
  11. Mo, F.T., Zheng, J.X., Xu, G.Y., Yang, N. (2011). Screening of SNP polymorphisms in the partial region of the FMO3 gene [C]. China Animal Husbandry and Veterinary Society Poultry Society. 270-275. 
  12. Zhang, X.H., Pang, Y.Z., Zhao, S.J., Xu, H.W., Li, Y.L. , Xu , Y., Guo, Z. and Wang, D.D.(2013). The relationship of plumage colours with MC1R (Melanocortin 1 Receptor) and ASIP (Agouti Signaling Protein) in Japanese quail (Coturnix coturnix japonica). British Poultry Science. 54(3): 306-311.
  13. Zhong,H.,Luo,Y.,Sun,J.,Wang,C.,Wang,Q.G.,Gao,G.L.,Zhang,K.S.,Li,Q.,Wang,H.W.,Li,J., Chen, M.J., Wang, Y.M., Zhao, X.Z. (2017). Goose FMO3 gene cloning, tissue expression profiling polymorphism detection and association analysis with trimethylamine level in the egg yolk. Gene. 632: 25-35. 
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Indian Journal of Animal Research
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    Research Article

    volume 54 issue 9 (september 2020) : 1176-1178,   Doi: 10.18805/ijar.B-1168

    Polymorphism Analysis of FMO3 Gene in Two Strains Quails

    J
    Jun Yan Bai
    X
    Xiao Ping Jia1
    Y
    You Bing Yang
    G
    Guang Lu Li
    H
    Hong Deng Fan
    H
    Heng Cao
    X
    Xue Yan Fu
    K
    Kun Peng Shi
    1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. 
    Cite article:- Bai Yan Jun, Jia1 Ping Xiao, Yang Bing You, Li Lu Guang, Fan Deng Hong, Cao Heng, Fu Yan Xue, Shi Peng Kun (2019). Polymorphism Analysis of FMO3 Gene in Two Strains Quails. Indian Journal of Animal Research. 54(9): 1176-1178. doi: 10.18805/ijar.B-1168.

    ABSTRACT

    In this study, polymorphism of FMO3 genes in French giant quail and Savimit quail was tested by PCR technology and hybrid DNA pool sequencing technology. The results show that, one SNPs mutation site (A66G) was detected from the exon 2 of FMO3, the A and G allele frequencies of A66G in French giant quail and Savimit quail were 0.586/0.414 and 0.395/0.605. Two SNPs mutation sites (A139T and C187T) were detected from the exon 4 of FMO3, A and T allele frequencies of A139T in French giant quail and Savimit quail were 0.630/0.370 and 1.000 /0.000, C and T allele frequencies of C187T in French giant quail and Savimit quail were 0.256/0.744 and 0.763/0.237, respectively. Two mutation sites (A39G and A41T) were detected from exon 7, A and G allele frequencies of A39G in French giant quail and Savimit quail were 0.769/0.231 and 0.190 /0.810, A and T allele frequencies of A41T were 0.107/0.893 and 0.000/1.000. One SNPs mutation site (C219T) was detected from exon 9, C and T allele frequencies of C219T in French giant quail and Savimit quail were 0.250/0.750 and 0.250/0.750, respectively. According to clustering analysis, 10 different livestock species were finally clustered into three groups. Quail had close genetic relationship with jungle fowl and they were clustered into one group. The tropical xenopus was clustered into the second group. The rest animals were clustered into the third group. 

    REFERENCES

    1. Bai, J.Y., Jia, X.P., Yang, Y.B., Pang, Y.Z. and Wang, Y.Q. (2017b). Study on the polymorphism of fibroblast growth facor receptor (FGFR1) in sheep. Indian J. Anim. Res. 51: 856-859.
    2. Bai, J.Y., Wang, X., Yang, Y.B., Zhang, X.H., Pang, Y.Z. and Li, H.W. (2016e). Study on the polymorphism of POU1F1 gene in sheep. R. Bras. Zootec. 45: 604-607. https://doi.org/ 10.1590/S1806- 92902016001000004.
    3. Bai, J.Y., Zhao, Y.G., Li, G.L., Yang, Y.B., Wang, Y.Q. , Wang ,X. and Yang, S. (2019). Analysis of polymorphism of growth hormone secretagogue receptor in goat. Indian Journal of Animal Research. 53(7): 856-859.
    4. Bai,J.Y., Pang,Y.Z.,Qi,Y.X., Zhang,X.H. and Yun,Y.X.(2017a). Analysis of the polymorphism of expressed sequence tag-Simple sequence repeat in quail. Indian Journal of Animal Research. 51(5): 851-855. DOI:10.18805/ijar.v0i0f.3803
    5. Bai,J.Y., Pang,Y.Z.,Wu,S.J., Yu,M.Q. and Zhang,X.H.(2016a). Analysis of genetic diversity of four quails by microsatellite markers. Indian Journal of Animal Research. 50(1): 1-7. DOI: 10.18805/ ijar.8429
    6. Bai,J.Y., Pang, Y.Z., Zhang, X.H., Yun, Y.X. and Qi, Y.X.(2016b). Microsatellite Analysis of Genetic Diversity in Quail Populations from China. Brazilian Journal of Poultry Science. 18(3): 519-524. http://dx.doi.org/ 10.1590 /1806-9061-2015-0101.
    7. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, X.Y. (2016c). Development and application of Est-Ssr Markers in quails, Brazilian Journal of Poultry Science. 18(Special Issue):027-032. http://dx.doi.org/10.1590/1806-9061-2015-0124.
    8. Bai, J.Y., Pang, Z., Zhang, X.H. and Li, Y.X. (2016d). Study on the Morphological Development of quail Embryos.Brazilian Journal of Poultry Science, 18(Special Issue2):033-036. http://dx.doi.org/10.1590/1806-9061-2015- 0177. 
    9. Li, Y.X., Zhang, X.H., Pang, Y.Z., Qi, Y.X. and Zhao, S.J. (2019). Construction of MC1R and ASIP Eukaryotic expression vector and its regulation of plumage color in Japanese quail (Coturnix japonica). Journal of Poultry Science. 56(2): 84-90.
    10. Mo, F.T. (2014). Cloning and polymorphism of quail FMO3 gene associated with Trimethylamine content in Egg Yolk. Ph.D. Dissertation of China Agricultural University.
    11. Mo, F.T., Zheng, J.X., Xu, G.Y., Yang, N. (2011). Screening of SNP polymorphisms in the partial region of the FMO3 gene [C]. China Animal Husbandry and Veterinary Society Poultry Society. 270-275. 
    12. Zhang, X.H., Pang, Y.Z., Zhao, S.J., Xu, H.W., Li, Y.L. , Xu , Y., Guo, Z. and Wang, D.D.(2013). The relationship of plumage colours with MC1R (Melanocortin 1 Receptor) and ASIP (Agouti Signaling Protein) in Japanese quail (Coturnix coturnix japonica). British Poultry Science. 54(3): 306-311.
    13. Zhong,H.,Luo,Y.,Sun,J.,Wang,C.,Wang,Q.G.,Gao,G.L.,Zhang,K.S.,Li,Q.,Wang,H.W.,Li,J., Chen, M.J., Wang, Y.M., Zhao, X.Z. (2017). Goose FMO3 gene cloning, tissue expression profiling polymorphism detection and association analysis with trimethylamine level in the egg yolk. Gene. 632: 25-35. 
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