Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 9 (september 2020) : 1176-1178

Polymorphism Analysis of FMO3 Gene in Two Strains Quails

Jun Yan Bai, Xiao Ping Jia1, You Bing Yang, Guang Lu Li, Hong Deng Fan, Heng Cao, Xue Yan Fu, Kun Peng Shi
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. 
Cite article:- Bai Yan Jun, Jia1 Ping Xiao, Yang Bing You, Li Lu Guang, Fan Deng Hong, Cao Heng, Fu Yan Xue, Shi Peng Kun (2019). Polymorphism Analysis of FMO3 Gene in Two Strains Quails. Indian Journal of Animal Research. 54(9): 1176-1178. doi: 10.18805/ijar.B-1168.

ABSTRACT

In this study, polymorphism of FMO3 genes in French giant quail and Savimit quail was tested by PCR technology and hybrid DNA pool sequencing technology. The results show that, one SNPs mutation site (A66G) was detected from the exon 2 of FMO3, the A and G allele frequencies of A66G in French giant quail and Savimit quail were 0.586/0.414 and 0.395/0.605. Two SNPs mutation sites (A139T and C187T) were detected from the exon 4 of FMO3, A and T allele frequencies of A139T in French giant quail and Savimit quail were 0.630/0.370 and 1.000 /0.000, C and T allele frequencies of C187T in French giant quail and Savimit quail were 0.256/0.744 and 0.763/0.237, respectively. Two mutation sites (A39G and A41T) were detected from exon 7, A and G allele frequencies of A39G in French giant quail and Savimit quail were 0.769/0.231 and 0.190 /0.810, A and T allele frequencies of A41T were 0.107/0.893 and 0.000/1.000. One SNPs mutation site (C219T) was detected from exon 9, C and T allele frequencies of C219T in French giant quail and Savimit quail were 0.250/0.750 and 0.250/0.750, respectively. According to clustering analysis, 10 different livestock species were finally clustered into three groups. Quail had close genetic relationship with jungle fowl and they were clustered into one group. The tropical xenopus was clustered into the second group. The rest animals were clustered into the third group. 

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